Isoenzymes of wheat o-diphenolase revealed by column isoelectric focusing

Interesse, Francesco S.; Ruggiero, P.; Lamparelli, Francesco; D'Avella, Gerolmina

doi:10.1007/bf01042413

Cited by 10 publications

(13 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GPC of sorghum peroxidase (1.11.1.7) on Sephadex G 100 resulted in the elution of two peaks, while three peaks were obtained from Agarose A-15m (23). Four different forms of barley /3-anylase (24) and five isoforms of radish peroxidase (25) and wheat o-diphenolases (1.14.18.1) (19,23,26) were distinguished by separation on Sephadex G 75.…”

Section: Chromatographie Methodsmentioning

confidence: 99%

“…Changes of peroxidase and esterase patterns during anther development (16), and isoenzymes of diphenolase of wheat (19) and other plants (66)(67)(68)(69), were observed by IEF and SDS-PAGE.…”

Section: Electrophoretic Methodsmentioning

confidence: 99%

“…These results corespond with conclusions of other authors (17,(27)(28)(29)(30)(31). DEAE-cellulose chromatography was used, after column isoelectric focusing of wheat o-diphenolase, to obtain four enzymatically active fractions (19). Lipoxygenases (1.13.11.2) of soybean (32), wheat flour (12), and rice embryos (33) were separated and studied on DEAE-Sepharose, DEAE-and CM-cellulose, and DEAE-Sephacel columns, respectively.…”

Section: Chromatographie Methodsmentioning

confidence: 99%

“…Interesee and other authors (19,78,79) used 4-methylcatechol as a substrate for the determination of o-diphenolase (o-diphenol: Cvoxidoreductase, 1.10.3.1) in wheat.…”

Section: Photometric Determination With Natural Dye-labeled and Synmentioning

confidence: 99%

“…It was found that the stability of both enzymes was considerably increased by adding cysteine, 2-mercaptoethanol or dithiothreitol to the extraction buffer, but by far the best stabilizer was bovine serum albumin. The next step in isolation is usually precipitation of the active protein from the crude extract, customarily accomplished with cold organic solvents (17) or ammonium sulfate (18)(19)(20). The precipitate may then be sedimented, centrifuged, or filtered.…”

Section: Isolation Of Enzymes From Plant Sourcesmentioning

confidence: 99%

See 4 more Smart Citations

Some methods for isolation and assays of enzymes occurring in cereals and legumes

Filova

Valentová

Daussant³

et al. 1992

Food Reviews International

View full text Add to dashboard Cite

This review deals with isolation techniques and enzymatic assays which are applicable to the study of enzymes occurring in cereals and legumes. In the section of the paper concerned with separation and purification methods, various types of Chromatographic and electrophoretic techniques are described and specific examples are presented. The discussion of assay methods cites selected assay methods suitable for monitoring enzyme activities in plants using natural, synthetic, dye-labeled or radiolabeled substrates in different technical combinations. Immunochemical assays of enzymes, characterization of antibodies, and practical examples illustrating how these techniques can be used are also presented.

show abstract

Section: Chromatographie Methodsmentioning

confidence: 99%

Section: Electrophoretic Methodsmentioning

confidence: 99%

Section: Chromatographie Methodsmentioning

confidence: 99%

“…Interesee and other authors (19,78,79) used 4-methylcatechol as a substrate for the determination of o-diphenolase (o-diphenol: Cvoxidoreductase, 1.10.3.1) in wheat.…”

Section: Photometric Determination With Natural Dye-labeled and Synmentioning

confidence: 99%

Section: Isolation Of Enzymes From Plant Sourcesmentioning

confidence: 99%

See 3 more Smart Citations

Some methods for isolation and assays of enzymes occurring in cereals and legumes

Filova

Valentová

Daussant³

et al. 1992

Food Reviews International

View full text Add to dashboard Cite

show abstract

Objective Evaluation of Noodles

Hatcher¹

2010

Asian Noodles

View full text Add to dashboard Cite

Übersichtsartikel Sonnenblumensamen und deren Verarbeitungsprodukte 1. Mitt. Aufkommen und wertbestimmende Bestandteile

Mieth

Lange

Brückner

1984

Nahrung

View full text Add to dashboard Cite

Die Sonnenblumenpflanze besteht zu etwa 55 % aus Stengel-und Blattmaterial, 30 p/, Korben und 15 7, Samen. Letztere (Achenen) setzen sich aus Schalen, Samenhautchen und Kernen zusammen. In den letzten Jahren ist es durch Ziichtung, insbesondere in der Sowjetunion, gelungen, den Schalenanteil von 45-50 7; auf 22-25 % zu senken und gleichzeitig den Olgehalt auf etwa 50 % zu erhohen (Tab. 3).Wertbestimmende Bestandteile von Sonnenblumensamen sind neben Olen vorrangig Proteine. Daruber hinaus enthalten diese betrachtliche Mengen an Mineralstoffen (0,36 Calcium, 0,60% Phosphor) und sind reich an B-Vitaminen (pro 100 g 1,s mg Thiamin, 3 1,2 mg Nicotinsaure, 0,47 mg Riboflavin). Entfettete Sonnenblumenkerne sind hinsichtlich des Nicotinsaure-und Pantothensauregehaltes Sojamehlen sowie Mais-und Weizenkeimen uberlegen [40, 141, 1701. Als wertmindernd sind dagegen hauptsachlich Phenolsauren und Kohlenhydrate anzusehen. Kerne bestehen uberwiegend aus Nahrstoffen, insbesondere Lipiden, Proteinen, Monound Oligosacchariden. Hauptbestandteile der Schalen sind unlosliche, unverdauliche Polysaccharide (Rohfaser) (Tab. 4 und 5). Pro teineAllgemeine Betrachtungen. Proteine sind uberwiegend in den Aleuronkornern der Sonnenblumensamen lokalisiert, wobei der durchschnittliche Proteingehalt der Samen in Abhangigkeit von genetischen und anderen EinfluBfaktoren zwischen 15 und 22 % liegt und meistens negativ mit dem Fettgehalt korreliert [34, 130, 163,201, 2041. Bereits 1880 konnte von RITTHAUSEN [ 1761[ , 1898 [ 1471 nachgewiesen werden, da13 sich diese aus strukturell verschiedenartigen Fraktionen zusammensetzen. Sie lassen sich daher aufgrund unterschiedlicher Molmassen und Basizitat, durch ihr Verhalten bei der Ultrafiltration, Chromatographie und Elektrophorese sowie durch ihre Extrahierbarkeit in verschiedenen Losungsmitteln voneinander trennen [8, 18, 69, 87, 92, 186, 206, 212, 214, 216,

show abstract

Isoenzymes of wheat o-diphenolase revealed by column isoelectric focusing

Cited by 10 publications

References 25 publications

Some methods for isolation and assays of enzymes occurring in cereals and legumes

Some methods for isolation and assays of enzymes occurring in cereals and legumes

Objective Evaluation of Noodles

Übersichtsartikel Sonnenblumensamen und deren Verarbeitungsprodukte 1. Mitt. Aufkommen und wertbestimmende Bestandteile

Contact Info

Product

Resources

About