Isoenzymes of N-acetyl-beta-hexosaminidase in complicated pregnancy.

Arciuch, L.; Bielecki, Dariusz; Borzym, Małgorzata; Południewski, G; Arciszewki, Krzysztof; Różański, Andrzej; Zwierz, Krzysztof

doi:10.18388/abp.1999_4122

Cited by 10 publications

(3 citation statements)

References 1 publication

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“…Degranulation, detected by the release of β-hexosaminidase, have been associated with the injection of DENV in other studies [19]. The cleavage of some substrates of this enzyme have been associated with NKT cell differentiation, and the high activity of β-hexosaminidase has already been observed in placental dysfunctions [35,38].…”

Section: Discussionmentioning

confidence: 90%

Análise Dos Fatores De Prevenção Do Câncer Ginecológico

Pereira¹,

Pereira²,

Vasconcelos³

et al. 2020

Ações De Saúde E Geração De Conhecimento Nas Ciências Médicas 4

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Direitos para esta edição cedidos à Atena Editora pelos autores. Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, inclusive não representam necessariamente a posição oficial da Atena Editora. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais. A Atena Editora não se responsabiliza por eventuais mudanças ocorridas nos endereços convencionais ou eletrônicos citados nesta obra.

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Section: Discussionmentioning

confidence: 90%

Análise Dos Fatores De Prevenção Do Câncer Ginecológico

Pereira¹,

Pereira²,

Vasconcelos³

et al. 2020

Ações De Saúde E Geração De Conhecimento Nas Ciências Médicas 4

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“…Degranulation, detected by the release of β-hexosaminidase, has been associated with the injection of DENV in other studies [19]. The cleavage of some substrates of this enzyme has been associated with NKT cell differentiation, and the high activity of β-hexosaminidase has already been observed in placental dysfunction [35,38].…”

Section: Discussionmentioning

confidence: 91%

Zika Virus Infects Human Placental Mast Cells and the HMC-1 Cell Line, and Triggers Degranulation, Cytokine Release and Ultrastructural Changes

Rabelo

Gonçalves

Souza

et al. 2020

Cells

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Zika virus (ZIKV) is an emergent arthropod-borne virus whose outbreak in Brazil has brought major public health problems. Infected individuals have different symptoms, including rash and pruritus, which can be relieved by the administration of antiallergics. In the case of pregnant women, ZIKV can cross the placenta and infect the fetus leading to congenital defects. We have identified that mast cells in the placentae of patients who had Zika during pregnancy can be infected. This led to our investigation on the possible role of mast cells during a ZIKV infection, using the HMC-1 cell line. We analyzed their permissiveness to infection, release of mediators and ultrastructural changes. Flow cytometry detection of ZIKV-NS1 expression 24 h post infection in 45.3% of cells showed that HMC-1 cells are permissive to ZIKV infection. Following infection, β-hexosaminidase was measured in the supernatant of the cells with a notable release at 30 min. In addition, an increase in TNF-α, IL-6, IL-10 and VEGF levels were measured at 6 h and 24 h post infection. Lastly, different intracellular changes were observed in an ultrastructural analysis of infected cells. Our findings suggest that mast cells may represent an important source of mediators that can activate other immune cell types during a ZIKV infection, which has the potential to be a major contributor in the spread of the virus in cases of vertical transmission.

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“…Both isoenzymes recognize terminal N-acetylglucosamine and Nacetylgalactosamine, but only isoenzyme A recognizes 6-sulfated residues of these sugars (Winchester, 1996). HEX is active in most tissues and organs such as kidney (Skalova, 2005), spleen (Emiliani et al, 1999), liver (Elsafi et al, 1994), mucous membrane of stomach and intestine (Gil-Martin et al, 1999;Michaels & Hellequist, 2001), cortex (Hammarsund et al, 2004), lung (Baritussio et al, 2001;Minami et al, 1985), epidermal fibroblasts (Ichisaka et al, 1998), placenta (Arciuch et al, 1999) and neoplastic tissues (Bhuvarahamurthy & Govindsamy, 1996;Lerner, 1996;Matsuura et al, 2004). HEX is used as a marker for monitoring the course of sereval diseases like chronic glomerulonephritis (Bazzi et al, 2002), urinaemia (Linko-Lopponen, 1986;Stabellini et al, 2005), arterial hypertension (Perez-Blanco, 1996), Sjogren's syndrom (Sohar et al, 2005), advanced stadium of diabetes (Nakazawa & Tamai, 1991), rheumatoid arthritis (Popko et al, 2005), idiopathic juvenile arthritis (Popko et al, 2003), osteoarthritis (Liu et al, 2004) and for evaluation of kindney function after transplantation (Kotanko et al, 1996).…”

Section: E Olszewska and Othersmentioning

confidence: 99%

Role of N-acetyl-beta-d-hexosaminidase in cholesteatoma tissue.

Olszewska¹,

Olszewski²,

Borzym‐Κluczyk³

et al. 2007

Acta Biochim Pol

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Cholesteatoma is a destructive disease characterized by the progressive expansion of keratinizing squamous epithelium in the middle ear and mastoid, and chronic inflammatory reaction of the subepithelial connective tissue. N-Acetyl-beta-d-hexosaminidase (HEX) catalyzes the release of terminal non-reducing N-acetyl-d-hexosamine residues acting on glucosides and galactosides in glycoproteins, G(M2)-gangliosides and glycosaminoglycans (GAGs). In this study the activities of HEX were measured in cholesteatoma tissue and in normal skin to demonstrate a possible role of HEX in bone resorption in the area adjacent to cholesteatoma. Cholesteatomas (n = 21) and normal adult retroauricular skin (controls, n = 21), were collected from patients during surgery due to chronic otitis media. In 20 of 21 specimens a significantly higher activity of HEX was observed in cholesteatoma tissue compared with that in normal skin. Mean release of HEX from the activated cells was 68.55 +/- 30.77 nkat/g wet tissue in cholesteatoma and 31.79 +/- 10.02 nkat/g wet tissue in skin specimens. It may explain the process of bone resorption in the area adjacent to cholesteatoma, i.e. ossicles or temporal bone. This study suggests that drugs inhibiting HEX activity, such as iminocyclitols, may be useful in cholesteatoma treatment.

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Isoenzymes of N-acetyl-beta-hexosaminidase in complicated pregnancy.

Cited by 10 publications

References 1 publication

Análise Dos Fatores De Prevenção Do Câncer Ginecológico

Análise Dos Fatores De Prevenção Do Câncer Ginecológico

Zika Virus Infects Human Placental Mast Cells and the HMC-1 Cell Line, and Triggers Degranulation, Cytokine Release and Ultrastructural Changes

Role of N-acetyl-beta-d-hexosaminidase in cholesteatoma tissue.

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