Isoenzymes of lactate dehydrogenase in human leukemic cells in culture treated with inducers of differentiation.

Abstract

The human leukemic cell lines HL60 and K562, were induced to differentiate terminally by chemical agents . The isoenzyme patterns of lactate dehydrogenase (LD) in the cells before and after differentiation were determined electrophoretically on agarose gels . In general, treatment of the leukemic cells with inducers of differewl 'r .in resulted in a quantitative shift of the isoenzyme pattern towards anodic or cathodic forms. This was correlated with the conversion of the chemically treated cells to morphologically more normal cells, as verified by light microscopy and/or synthesis of hemoglobin . The LID isoenzyme patterns of the chemically differentiated cells were : (a) characteristic for the particular cell type obtained rather than for the nature of the inducer used ; and (b) not similar to those of normally differentiated cells of the corresponding lineage, indicating that incomplete differentiation had occurred .

Regulation of the expression of lactate dehydrogenase isozymes in human lymphocytes

“…2). However, the major change is in LDH-A, which is consistent with the idea that it is the A subunit which is regulated [1][2][3][4].…”

“…Expression of the A subunit is tissue-specific and developmentally regulated. In neoplastic or transformed lymphoid cells, LDH-A is reported to be a differentiation marker which is progressively expressed during maturation along the T-blast axis [1][2][3] and during terminal differentiation of myeloid cell lines [4]. In activated peripheral lymphoid cells, increased LDH-A activity appears to play a role in glucose metabolism.…”

α1,3-L-Fucosyltransferase Expression in Developing Human Myeloid Cells

“…The surface glycoprotein (18,19) and glycolipid profiles (20,21) of the cells specifically change during differentiation. Therefore, although granulocytic maturation of HL-60 cells has been shown to be defective in some respects (20,(22)(23)(24)(25), this cell line offers a useful model for studying the enzymatic basis of changes that take place in expression of carbohydrate cell surface structures during differentiation of myeloid cells. Our previous studies demonstrated that Me 2 SO-induced differentiation of HL-60 cells resulted in a lowering of ␣1,3-fucosyltransferase and ␣2,6-sialyltransferase activities, an increase in cell surface expression of sialyl-Le x , and a loss of the marked preference for non-sialylated acceptors shown by the ␣1,3-fucosyltransferase(s) in undifferentiated cells (14).…”

Effect of Granulocyte-Colony-Stimulating Factor on Serum Lactate Dehydrogenase Levels and Isoenzymes in a Rabbit Model

“…The distribution of isoenzymes when LDH is augmented is dependent on the cells from which they originated [13]. During hematopoiesis, hematopoietic cells express a different LDH isoenzyme profile according to their differentiation stage [14]. In a study using CML K562 cells, it was demonstrated that while blast cells carried an LDH5-like isoenzyme, the more mature cells did not [15].…”