Isoenzymes and protein polymorphism in Blaps polycresta and Trachyderma hispida (Forsskål, 1775) (Coleoptera: Tenebrionidae) as biomarkers for ceramic industrial pollution

Kheirallah, Dalia A.; El-Samad, Lamia M.

doi:10.1007/s10661-019-7517-x

Cited by 8 publications

(3 citation statements)

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“…X-ray analysis is an effective tool for detecting trace metal in biological specimens [ 15 , 50 ]. Our results align with previous studies that reported the toxic effect of heavy metals on aquatic and terrestrial insects collected from industrial areas [ 14 , 37 , 49 – 52 ]. Azam et al (2015) [ 51 ] stated that the elevation in heavy metals percentages in animal bodies correlates site pollution.…”

Section: Discussionsupporting

confidence: 93%

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Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt

et al. 2021

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The present research aims to evaluate the impact of industrial processes and anthropogenic activities on the beetle Pimelia latreillei inhabiting the polluted site at Zawya Abd El- Qader, Alexandria, Egypt. Beetles were collected from the vicinity of five factories. The genotoxic effects of environmental exposures to industrial heavy metals were monitored using a broad range of assays, including energy-dispersive X ray microanalysis and X-ray diffraction (SEM and EDX)), qRT-PCR gene expression assay, micronuclei formation, and transmission electron microscope (TEM). Energy dispersive X-ray microanalysis for the soil and testicular tissues of beetles collected from the polluted site revealed a higher percentage of heavy metals than the beetles collected from the reference site (Sidi Kirier, Alexandria, Egypt). To analyze/monitor genotoxicity in P. latreillei sampled from the polluted site, the transcription levels of levels of heat shock proteins (Hsps) and accessory gland seminal fluid protein (AcPC01) in testicular tissues were recorded. The incidence of micronuclei (MN) formation in the testicular cells was also observed. Quantitative RT-PCR (RT-qPCR) analysis was carried out to detect the changes in the gene expression of the aforementioned proteins. Genes encoding heat shock proteins (Hsp60, Hsp70, and Hsp90) were significantly overexpressed (> 2-fold) in specimens sampled from the polluted site; however, AcPC01 gene expression was under-expressed (<1.5-folds). The incidence of MN was significantly increased in specimens sampled from the polluted site. Ultrastructure anomalies (nuclear and cytoplasmic disruption) were also observed in the testicular cells of the beetles sampled from the polluted site compared to those sampled from the unpolluted site. Our results, therefore, advocate a need for adequate measures to reduce increasing environmental pollution in the urban-industrial areas.

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Section: Discussionsupporting

confidence: 93%

“…Employing insects in biomonitoring program is a functional ecological indication [ 12 , 14 , 15 , 35 , 37 , 48 , 49 ]. In the present study, the urban site is prone to industrial heavy metal pollution that might be released from the local factories.…”

Section: Discussionmentioning

confidence: 99%

Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt

et al. 2021

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“…In the study, Ni was detected by X-ray microanalysis in the midgut tissues of the treated beetles. X-ray microanalysis is a valid tool for illustrating the distribution of metals in biological tissues [46]. The small size, great capacity, and high reactivity of NPs facilitate their transportation to cellular organelles and exert harmful effects, unusual in their micron-sized counterparts [44].…”

Section: Plos Onementioning

confidence: 99%

Biochemical and histological alterations induced by nickel oxide nanoparticles in the ground beetle Blaps polychresta (Forskl, 1775) (Coleoptera: Tenebrionidae)

et al. 2021

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The present study evaluates the effect of nickel oxide nanoparticles on some biochemical parameters and midgut tissues in the ground beetle Blaps polychresta as an indicator organism for nanotoxicity. Serial doses of the NiO-NPs colloid (0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 mg/g) were prepared for injecting into the adult beetles. Insect survival was reported daily for 30 days, and the sublethal dose of 0.02 mg/g NiO-NPs was selected for the tested parameters. After the treatment, nickel was detected in the midgut tissues by X-ray microanalysis. The treated group demonstrated a significant increase in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities when compared to the untreated group. However, the treated group demonstrated a significant decrease in ascorbate peroxidase (APOX) activity when compared to the untreated group. Histological and ultrastructural changes in the midgut tissues of treated and untreated beetles were also observed. The current findings provide a precedent for describing the physiological and histological changes caused by NiO-NPs in the ground beetle B. polychresta.

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Migration of Copper with Wheat Straw in Soil under the Alternate Dry-Wet and Eluviation Conditions

Guo

Zhu

et al. 2019

Communications in Soil Science and Plant Analysis

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Isoenzymes and protein polymorphism in Blaps polycresta and Trachyderma hispida (Forsskål, 1775) (Coleoptera: Tenebrionidae) as biomarkers for ceramic industrial pollution

Cited by 8 publications

References 50 publications

Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt

Relative gene expression, micronuclei formation, and ultrastructure alterations induced by heavy metal contamination in Pimelia latreillei (Coleoptera: Tenebrionidae) in an urban-industrial area of Alexandria, Egypt

Biochemical and histological alterations induced by nickel oxide nanoparticles in the ground beetle Blaps polychresta (Forskl, 1775) (Coleoptera: Tenebrionidae)

Migration of Copper with Wheat Straw in Soil under the Alternate Dry-Wet and Eluviation Conditions

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