Isoenzyme studies in human leukemia — II. Carboxylic esterase (E.C. 3.1.1.1.)

Drexler, Hans; Gaedicke, Gerhard

doi:10.1016/0145-2126(83)90131-5

Cited by 21 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…or ampholyte pH 3-10 (Pharmacia-Sigma, St Louis, MO), 0.1 per cent (v/v) tetramethyl ethylenediamine. Separation was performed for 1 h at 5 "C, constant power of 30 W (Drexler and Gaedicke, 1983a).…”

Section: Separation Into Isoenzymesmentioning

confidence: 99%

Erythroleukemia cell lines hel and K‐562: Changes in isoenzyme profiles and morphology during induction of differentiation

Drexler

Gaedicke

Minowada

1986

Hematological Oncology

Self Cite

View full text Add to dashboard Cite

The effects of the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and of hemin on the human erythroleukemia cell lines HEL and K-562 were studied using the parameters cellular morphology, cell proliferation, positivity in the nitro-blue tetrazolium (NBT)-reduction test, adherence to surface, development of pseudopodia, and isoenzyme profiles of carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase (LDH) separated by isoelectric focusing on horizontal polyacrylamide gels. TPA and hemin induced similar morphological changes in HEL and K-562, erythrocyte-like cells were seen to be the predominant population after exposure to hemin. Both TPA and hemin led to cessation of cell proliferation and to a dose-dependent decrease in the number of viable nucleated cells. Whereas HEL cells treated with TPA became positive in the NBT-reduction test, adhered to plastic surface and showed pseudopodia, HEL cells exposed to hemin and K-562 cells cultured with TPA or hemin did not show these markers indicative of the macrophage-cell lineage. TPA-treated HEL cells exhibited isoenzyme patterns which were characteristically seen in cells of the monocyte-macrophage complex. 'Myeloid isoenzyme profiles' were found in TPA-treated K-562 cells. As evidenced by the above described phenotypical changes, HEL cells appeared to differentiate along the macrophage and erythroid cell lineages. Induced K-562 cells displayed myeloid and erythrocytic markers.

show abstract

Section: Separation Into Isoenzymesmentioning

confidence: 99%

Erythroleukemia cell lines hel and K‐562: Changes in isoenzyme profiles and morphology during induction of differentiation

Drexler

Gaedicke

Minowada

1986

Hematological Oncology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the cells did not express an esterase isoenzyme that is specific for monocytes and macrophages [13]. Taking these results together, a differentiation to monocytes and macrophages appears to be excluded.…”

Section: Discussionmentioning

confidence: 95%

“…for esterase, acid phosphatase, LDH, and ampholyt of pH 3-10 (Sigma, St. Louis, MO) for hexosaminidase, 0.1% (v/v) temed. Runs were carried out for 1 hr at a constant power of 30 W, current decreasing from 60 to 15 mA, voltage up to 1,400 V, cooling at 5°C [13]. Isoenzymes were visualized directly on the gels.…”

Section: Lsoelectric Focusingmentioning

confidence: 99%

Induction of differentiation in the human leukemia cell line SPI‐802: Morphological, immunological, and isoenzymatic changes

et al. 1986

Self Cite

View full text Add to dashboard Cite

The phorbolester 12-0-tetradecanoylphorbol 13-acetate (TPA) was used for the induction of differentiation in cells of the human leukemia cell line SPI-802. The cellular morphology, surface marker antigen expression, and isoenzyme profiles of four enzymes (carboxylic esterase, acid phosphatase, hexosaminidase, and lactate dehydrogenase) served as parameters for monitoring the induced phenotypical changes. TPA led to distinct alterations of the morphology and significantly affected the growth rate with cessation of cell proliferation. No major increase in the number of nitro blue tetrazolium-positive cells or aggregation of cells, phagocytosis of latex beads, adherence to plastic surface, or development of pseudopodia were observed. As TPA-treated SPI-802 cells remained negative for these markers of the monocyte-macrophage complex, it can be concluded that the cells did not differentiate into monocytes and macrophages. The immunological marker profile based on testing of 55 monoclonal antibodies, terminal deoxynucleotidyl transferase and two erythrocyte rosette tests indicated a differentiation of SPI-802 cells along the granulocytic cell lineage. This was confirmed by isoenzyme analysis, especially that of carboxylic esterase. An isoenzyme specific for monocytes and macrophages was not detected. In earlier studies it was found that SPI-802 cells produce hemoglobin upon exposure to TPA or hemin. This latter observation and the present results suggested a comparison with the two erythroleukemia cell lines K-562 and HEL. SPI-802 cells appear to have the potential to differentiate along several cell lineages.

show abstract

“…Isoenzyme analysis using the enzyme carboxylic esterase (E.C.3.1.1.1) was performed routinely on all specimens as described in detail elsewhere [6].…”

Section: Methodsmentioning

confidence: 99%

“…Using one particular enzyme, the carboxylic or nonspecific esterase (E.C.3.1.1.1), one can discriminate leukemia cells of "pure myelocytic" origin from those with monoblastic nature: one isoenzyme which can be inhibited completely and selectively by sodium fluoride (NaF) (the "NaF-sensitive mono-band') is specific for monocytes [6,24,26].…”

Section: Introductionmentioning

confidence: 99%

Reactivity patterns of monoclonal antibodies positive on myelomonocytic leukemia cells as defined by esterase isoenzyme analysis

Drexler

Menon

Gaedicke

et al. 1986

Cancer Immunol Immunother

Self Cite

View full text Add to dashboard Cite

The reactivity with monoclonal antibodies (MoAbs) specific for myelomonocytic cells and the expression of a particular esterase isoenzyme were analyzed in 159 cases of acute myeloid leukemias. The incidence of positivity of 16 MoAbs (MCS-2, MCS-1, OKM1, My-1, Leu-M1, Leu-M3, CA-2-38, MY4, MY7, MY8, MY9, VIM-D2, VIM-D5, Mo1, Mo2, 63D3) was studied using the indirect immunofluorescence technique. A carboxylic esterase isoenzyme which can be inhibited completely and selectively by sodium fluoride (NaF) was demonstrated by isoelectric focusing on horizontal polyacrylamide gels. This NaF-sensitive isoenzyme indicated the monocytic origin of the blast cells as it is specific for this cell lineage. Prior to the immunological-isoenzymatic analysis all cases were categorized into two subtypes according to morphological criteria of the FAB classification system: 147 cases of AML (FAB M1-3) and 12 cases of AMMoL/AMoL (FAB M4/5). However, 15 out of 147 cases of AML expressed the NaF-sensitive isoenzyme and were therefore assigned to the group AMMoL/AMoL. Likewise, 1 case, diagnosed morphologically as AMMoL, was negative for this marker isoenzyme and was assigned to the other leukemia subtype. The incidence of reactivity varied widely for the MoAbs tested regarding the overall results on all cases and the positivity of cases of either AML or AM-MoL/AMoL. The MoAbs were grouped into four classes depending on the pattern of reactivity with myeloblastic or monoblastic or both subtypes of acute myeloid leukemia. The MoAbs MCS-2, MY7, Leu-M1, and MY9 detected the vast majority of cases with either myelocytic or monocytic involvement (group-I: "pan-myelomonocytic" reactivity). The MoAbs MCS-1, OKM1, VIM-D5, and Mo1 showed a predominance in their staining pattern for monocytic variants, but were also positive on a substantial percentage of nonmonocytic cases (group-II: predominantly reactive with monocytic, but also myelocytic cases). The MoAbs Leu-M3, MY4, VIM-D2, Mo2, and MY8 reacted with the large majority of AMMoL/AMoL cases and with a small number of AML cases (group-III: monocyte-"specific" reactivity). The MoAbs of group-I are useful in differentiating acute lymphoid from acute myeloid leukemias. The MoAbs of group-III, and to a lower extent those of group-II, will be of considerable value in the subtyping of acute myeloid leukemias. The results show that accuracy of leukemia classification might not always be achieved by morphology alone, but that immunological and biochemical aspects should be included as well, and several MoAbs are very useful tools for classification and subtyping of acute myeloid leukemias.

show abstract

Isoenzyme studies in human leukemia — II. Carboxylic esterase (E.C. 3.1.1.1.)

Cited by 21 publications

References 34 publications

Erythroleukemia cell lines hel and K‐562: Changes in isoenzyme profiles and morphology during induction of differentiation

Erythroleukemia cell lines hel and K‐562: Changes in isoenzyme profiles and morphology during induction of differentiation

Induction of differentiation in the human leukemia cell line SPI‐802: Morphological, immunological, and isoenzymatic changes

Reactivity patterns of monoclonal antibodies positive on myelomonocytic leukemia cells as defined by esterase isoenzyme analysis

Contact Info

Product

Resources

About