Isoenzyme biotypes of Candida species

Lehmann, Paul F.; Kemker, B. J.; Hsiao, Chiu-Bin; Dev, Soumyabrata

doi:10.1128/jcm.27.11.2514-2521.1989

Cited by 72 publications

(49 citation statements)

References 32 publications

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“…This information supports our idea that the high discrimination produced by the combination of all of the enzymes occurs because of the SDH and MDH band profiles, and these are mostly responsible for the distinction between isolates. This information also supports the individual, system-bysystem interpretation of results and does not support the conclusion of Lehmann et al [40] that the variability pattern of SDH is relevant only to C. albicans strain distinction. According to our results, it is possible to make an intra-specific distinction.…”

Section: Alleles Of Eight Enzyme Loci*contrasting

confidence: 87%

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Santos

Melo

Ponzzes

et al. 2011

Mycoses

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Twenty-eight Candida albicans strains obtained from women with vaginal candidiasis were tested for phospholipase and proteinase production and clustered by multilocus enzyme electrophoresis (MLEE). The proteolytic and phospholipidic activity were considered moderate (0.56 ± 0.12 mm and 0.53 ± 0.09 mm, respectively) for all isolates. The isoenzymes malate dehydrogenase (MDH) and sorbitol dehydrogenase (SDH) showed strong intra-specific discriminatory power. The numerical and genetic interpretation of the bands produced by the isoenzymes tested presented similar discriminatory power. The genetic diversity of the isolates was measured by allelic and genic frequency, perceptual index of polymorphic loci (P = 87.5%), average number of alleles per locus, average number of alleles per polymorphic locus, average heterozygosity observed and average heterozygosity expected. We verified that three isoenzymatic loci (Adh, Gdh and Sdh-2) were not in Hardy-Weinberg equilibrium. A dendrogram constructed based on the genetic distance matrix of Nei showed seven clusters; 57.15% (16) of the isolates were considered highly related or indistinguishable, and 42.85% were considered moderately related or unrelated. We did not find a relationship between the clusters and the exoenzymes production.

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Section: Alleles Of Eight Enzyme Loci*contrasting

confidence: 87%

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Santos

Melo

Ponzzes

et al. 2011

Mycoses

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“…About 200 g of protein was applied to native discontinuous polyacrylamide gels. After electrophoresis, the gels were washed twice in buffer and stained for enzyme activity, as described previously (15,18 A loopful of conidia was used to inoculate 40 ml of YPD broth (1% glucose, 1% yeast extract, 2% peptone) in a 250-ml Erlenmeyer flask. After incubation on a rotatory shaker (36 to 48 h, 37ЊC, 150 rpm), mycelia were harvested by filtration, washed once with sterile distilled water, and frozen in liquid nitrogen.…”

Section: Strains the 35mentioning

confidence: 99%

Comparison of three typing methods for clinical and environmental isolates of Aspergillus fumigatus

et al. 1995

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To evaluate procedures used for epidemiologic analysis of outbreaks of aspergillosis, we analyzed a collection of 35 Aspergillus fumigatus isolates using three typing methods: isoenzyme analysis (IEA), random amplified polymorphic DNA (RAPD) analysis, and restriction endonuclease analysis (REA). Twenty-one isolates were from a single hospital, with four isolates coming from different patients. Three clinical isolates came from a different hospital, and 11 clinical or environmental isolates were derived from a culture collection. With IEA, the patterns of alkaline phosphatase, esterase, and catalase discriminated nine types. In contrast, 22 types were obtained with five different RAPD primers, and 21 types could be detected with three of these (R108, R151, and UBC90). Restriction endonuclease analysis of genomic DNA, digested with either XbaI, XhoI, or SalI, detected 3, 17, and 13 different REA types, respectively, and 22 types were identified by combining the data from the XhoI and SalI REAs. Twenty-eight types were obtainable with a combination of REA, IEA, and RAPD patterns. Overall, the results pointed to substantial genetic variation among the isolates. Though two isolates had markedly distinct genotypes, their morphologic features and exoantigens were consistent with their being A. fumigatus. The analysis will help in planning epidemiologic studies of aspergillosis.

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“…Several methods have been used to fingerprint C. albicans, including electrophoretic karyotyping (2, 3, 5, 6, 12-14, 24, 27, 33, 67, 69), restriction fragment length polymorphism (RFLP) analysis (4,28,33,66,70), randomly amplified polymorphic DNA (RAPD) analysis (5,6,9,17,23,24,42,51), Southern blot hybridization with a variety of moderately repetitive DNA probes (11,20,25,29,31,46,57), and multilocus enzyme electrophoresis (MLEE) (7,8,10,21,22,38,41). However, in most of these studies the patterns generated by the fingerprinting methods were not characterized for their level of discrimination, and the methods were not verified by other unrelated methods for their capacity to measure genetic distance between independent isolates.…”

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confidence: 99%

Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans

et al. 1997

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Randomly amplified polymorphic DNA (RAPD) analysis, multilocus enzyme electrophoresis (MLEE), and Southern blot hybridization with moderately repetitive DNA probes have emerged as effective fingerprinting methods for the infectious fungus Candida albicans. The three methods have been compared for their capacities to identify identical or highly related isolates, to cluster weakly related isolates, to discriminate between unrelated isolates, and to assess microevolution within a strain. By computing similarity coefficients between 29 isolates from three cities within the continental United States, strong concordance of the results is demonstrated for RAPD analysis, MLEE, and Southern blot hybridization with the moderately repetitive probe Ca3, and weaker concordance of the results is demonstrated for these three fingerprinting methods and Southern blot hybridization with the moderately repetitive probe CARE2. All methods were also demonstrated to be able to resolve microevolution within a strain, with the Ca3 probe exhibiting the greatest resolving power. The strong correlations demonstrated between polymorphic markers assessed by the four independent fingerprinting methods and the nonrandom association between loci demonstrated by RAPD analysis and MLEE provide evidence for strong linkage disequilibrium and a clonal population structure for C. albicans. In addition, a synapomorphic allele, Pep-3A, was found to be present in all members of one of the three clusters discriminated by RAPD analysis, MLEE, and Ca3 fingerprinting, supporting the concordance of the clustering capacities of the three methods, the robustness of the clusters, and the clonal nature of the clusters.

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Isoenzyme biotypes of Candida species

Cited by 72 publications

References 32 publications

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Comparison of three typing methods for clinical and environmental isolates of Aspergillus fumigatus

Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans

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