Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm

Jahnke, Gizella; Májer, J.; Lakatos, Ákos; Molnár, J.; Deák, Edit; Stefanovits-Bányai, Éva; Varga, P.

doi:10.1016/j.scienta.2008.11.021

Cited by 17 publications

(11 citation statements)

References 29 publications

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“…To this end, polymerase chain reaction (PCR)-based DNA marker technologies have been widely applied in many crop species. In grapevine, SSRs (Simple Sequence Repeats) have been employed efficiently for cultivar identification (Bowers et al, 1999;Crespan, 2003;This et al, 2004;Jahnke et al, 2009;Zoghlami et al, 2009) and for clonal discrimination (Regner et al, 2000;Riaz et al, 2002;Bertsch et al, 2003;Crespan, 2003;Gonzàles-Techera et al, 2004;Hocquigny et al, 2004). On the contrary, some other researches have revealed the inability of SSRs to distinguish among grapevine clones Imazio et al, 2002;Vignani et al, 2002;Ibànez et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

Alba

Anaclerio

Gasparro³

et al. 2016

SAJEV

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To characterise 31 different Aglianico accessions randomly collected in Southern Italy, 30 ampelographic descriptors, 13 SSRs and 10 AFLP primer combinations were analysed. An appreciable variation of ampelographic descriptors was revealed mainly by mature leaf traits, while very few variations were recorded for shoot and berry traits. Similarly, all SSR loci revealed molecular monomorphism and AFLPs with a very high genetic similarity (Dice coefficient) among all the accessions considered. One of the aims of this study was to clarify the genetic assessment of Aglianico Nero and Aglianico del Vulture Nero, since they are registered as two different cultivars with distinct varietal codes at the Italian Register of Grape Varieties. Registered Aglianico Nero and Aglianico del Vulture Nero were included in the analyses, compared and used as reference material. Our plants showed that all the accessions tested, independent from the biotype, and the two registered cultivars belong to the same genotype, suggesting that, as reported by the Vitis International Variety Catalogue, a case of synonymy occurred between Aglianico Nero and Aglianico del Vulture Nero. These cultivars could therefore be considered as a single cultivar. Moreover, the AFLP data revealed a partial match between morphological and molecular data, showing that the AFLP molecular method was able to discriminate between different accessions belonging to the same cultivar.

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Section: Introductionmentioning

confidence: 99%

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

Alba

Anaclerio

Gasparro³

et al. 2016

SAJEV

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“…The results of the isoenzymatic and microsatellite analyses confirmed, that this two cultivars are different (Jahnke et al, 2007). Jahnke et al (2009) investigated the genetic diversity of Hungarian grapevine cultivars with biochemical and molecular markers (isoenzyme and SSR). The isoenzyme patterns of 4 enzyme systems (catechol-oxidase, glutamate-oxalacetate-transaminase, acid phosphatase and peroxidase) and the microsatellite profile in 6 loci (VVS2, VVS16, VVMD7, VMC4A1, VMC4G6, VrZag79) of 48 grapevine varieties were analysed.…”

Section: Isozyme Analysis In Grapementioning

confidence: 75%

Gel Electrophoresis of Grapevine (Vitis vinifera L.) Isozymes - A Review

Jahnke¹,

Májer²,

Remete³

2012

Gel Electrophoresis - Advanced Techniques

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“…Each forward primer of the primer pairs was fluorescently labelled with 6FAM on the 5′ end of the DNA chain. Separation of the amplified products was carried out through capillary electrophoresis in a PE-Applied Biosystems 3100 Automated Capillary DNA Sequencer; the molecular sizes of the products were determined using Peak Scanner Software (v. 1.0; Applied Biosystems) [8]. Allele sizes and peak area were also recorded for every single allele.…”

Section: Methodsmentioning

confidence: 99%

“…Allele sizes and peak area were also recorded for every single allele. Data were stored in Microsoft Excel [8].…”

Section: Methodsmentioning

confidence: 99%

Reconstruction of Parental SSR Haplotypes from a Single Grape Seed

Jahnke¹,

Nagy²,

Németh³

et al. 2019

Advances in Grape and Wine Biotechnology

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Microsatellite ('single sequence repeats' , SSR) markers were widely used in the last decade for the identification of parents of a given grapevine variety or for pedigree reconstruction as well. By now the pedigree of the majority of the most important varieties is established. At the same time, knowing both of the parents gives information about which one was the mother plant and which one was the pollinator. Analyses of archaeological grapevine seeds can give new opportunities in the research of the evolution of varieties. In most of the angiosperms, the endosperm is triploid with two genome equivalents from the maternal line and one from the paternal line. Our presumption was that this numeral difference in the maternal and paternal alleles causes measureable difference in the amplification of SSR alleles from grapevine seeds. To validate our method, pre-experiments were carried out on 12 'Pinot gris' seeds, which verified our theory.

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Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm

Cited by 17 publications

References 29 publications

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

Gel Electrophoresis of Grapevine (Vitis vinifera L.) Isozymes - A Review

Reconstruction of Parental SSR Haplotypes from a Single Grape Seed

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