Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid

Hofland, Gerard W.; Rijke, A. de; Thiering, Russell; Wielen, Luuk A.M. van der; Witkamp, Geert‐Jan

doi:10.1016/s0378-4347(00)00259-0

Cited by 58 publications

(59 citation statements)

References 23 publications

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“…The suitability of carbon dioxide as a volatile electrolyte in the precipitation of proteins was established for casein (Hofland et al, 1999;Jordan et al, 1987;Tomasula et al, 1995Tomasula et al, , 1997 as well as soy protein (Hofland et al, 2000). For soy protein, complete recovery of the acid precipitable protein mixture was achieved at a pH of 4.8-5.0 with the addition of carbon dioxide.…”

Section: Precipitation Using Volatile Electrolytesmentioning

confidence: 99%

“…For soy protein, complete recovery of the acid precipitable protein mixture was achieved at a pH of 4.8-5.0 with the addition of carbon dioxide. The use of carbon dioxide, proved to be advantageous for the particle morphology, giving spherical aggregates where conventional precipitation with sulfuric acid resulted in irregular shaped particles (Hofland et al, 2000). The influence of the protein concentration as well as the rate of acidification and stirrer speed on the particle size of soy protein was investigated as well.…”

Section: Precipitation Using Volatile Electrolytesmentioning

confidence: 99%

“…The relationship between carbon dioxide-head pressure and the pH of a soymeal extract, at equilibrium conditions is shown in Figure 4 for a 10 and 50 TWE% at 25°C (Hofland et al, 2000). The regions in which the glycinin (11S) and ␤-conglycinin (7S) fractions precipitate are highlighted for a 10 TWE% in Figure 4.…”

Section: Solution Acidity In Relation To Carbon Dioxide Pressurementioning

confidence: 99%

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Fractionation of soybean proteins with pressurized carbon dioxide as a volatile electrolyte

Thiering

Hofland

Foster

et al. 2001

Biotechnol. Bioeng.

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Section: Precipitation Using Volatile Electrolytesmentioning

confidence: 99%

Section: Precipitation Using Volatile Electrolytesmentioning

confidence: 99%

Section: Solution Acidity In Relation To Carbon Dioxide Pressurementioning

confidence: 99%

See 1 more Smart Citation

Fractionation of soybean proteins with pressurized carbon dioxide as a volatile electrolyte

Thiering

Hofland

Foster

et al. 2001

Biotechnol. Bioeng.

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“…It was thought that this approach might also be applicable to FO. Several examples of this approach are known in the literature, including processes to recover metals from aqueous solution [63], casein from milk [64,65], and soy protein from soy meal extract [66]. In our case, many of the reaction products from lignin depolymerization were phenolic in nature.…”

Section: Salts Of Carboxylic Acids and Carbon Dioxidementioning

confidence: 83%

“…Figure 22 illustrates several pertinent points regarding precipitation with CO 2 . Although the figure was adapted from some of the data from Hofland et al [66], the conclusions appear to apply generally to the literature. First, at lower pressures (< 150 psi), the pH is strongly dependent on the pressure.…”

Section: Salts Of Carboxylic Acids and Carbon Dioxidementioning

confidence: 99%

Forward osmosis :a new approach to water purification and desalination.

Miller¹,

Evans²

2006

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Fresh, potable water is an essential human need and thus looming water shortages threaten the world's peace and prosperity. Waste water, brackish water, and seawater have great potential to fill the coming requirements. Unfortunately, the ability to exploit these resources is currently limited in many parts of the world by both the cost of the energy and the investment in equipment required for purification/desalination. Forward (or direct) osmosis is an emerging process for dewatering aqueous streams that might one day help resolve this problem. In FO, water from one solution selectively passes through a membrane to a second solution based solely on the difference in the chemical potential (concentration) of the two solutions. The process is spontaneous, and can be accomplished with very little energy expenditure. Thus, FO can be used, in effect, to exchange one solute for a different solute, specifically chosen for its chemical or physical properties. For desalination applications, the salts in the feed stream could be exchanged for an osmotic agent specifically chosen for its ease of removal, e.g. by precipitation.This report summarizes work performed at Sandia National Laboratories in the area of FO and reviews the status of the technology for desalination applications. At its current state of development, FO will not replace reverse osmosis (RO) as the most favored desalination technology, particularly for routine waters. However, a future role for FO is not out of the question. The ability to treat waters with high solids content or fouling potential is particularly attractive. Although our analysis indicates that FO is not cost effective as a pretreatment for conventional BWRO, water scarcity will likely drive societies to recover potable water from increasingly marginal resources, for example gray water and then sewage. In this context, FO may be an attractive pretreatment alternative. To move the technology forward, continued improvement and optimization of membranes is recommended. The identification of optimal osmotic agents for different applications is also suggested as it is clear that the space of potential agents and recovery processes has not been fully explored.

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2000

Phytochem. Anal.

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Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid

Cited by 58 publications

References 23 publications

Fractionation of soybean proteins with pressurized carbon dioxide as a volatile electrolyte

Fractionation of soybean proteins with pressurized carbon dioxide as a volatile electrolyte

Forward osmosis :a new approach to water purification and desalination.

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