Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. IV. Further Studies on the Resolving Power in Connection with Separation of Myoglobins.

Vesterberg, Olof; Svensson, Harry; Nevald, R.; Frank, V.; Brunvoll, J.; Bunnenberg, E.; Djerassi, Carl; Records, Ruth

doi:10.3891/acta.chem.scand.20-0820

Cited by 1,300 publications

(231 citation statements)

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“…For determination of the isoelectric point, a sample of purified immunity protein was subjected to isoelectric focusing in a density gradient-stabilized natural pH gradient as described by Vesterberg and Svensson [9]. As shown in fig.…”

Section: Resultsmentioning

confidence: 99%

Purification and characterization of the cloacin DF13 immunity protein

Graaf

Klaasen-Boor

1974

FEBS Letters

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Section: Resultsmentioning

confidence: 99%

Purification and characterization of the cloacin DF13 immunity protein

Graaf

Klaasen-Boor

1974

FEBS Letters

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“…Isoelectric fractionation of the crude extract of the SMG was carried out according to the procedure of VESTERBERG and SVENSSON (1966). A sucrose gradient from 50 to 000 containing 100 Ampholine carrier ampholites was prepared in a 50 ml column for electrofocusing.…”

Section: Methodsmentioning

confidence: 99%

Androgen responsiveness of proteinase F in the submandibular salivary glands of inbred mice.

et al. 1987

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Using the radioimmunoassay technique, we studied androgenic regulations of proteinase F in the submandibular salivary glands (SMG) of various mouse strains (ICR, BALB/CA, C3H/HeN, C57BL/ION, and DBA/2N). The proteinase contents in BALB/CA and DBA/2N mice were almost the same between males and females. However, prominent sex differences in enzyme content were seen in all other strains; i.e., male glands of C3H/HeN and C57BL/ l ON contained more proteinase F than female glands did. On the contrary, the enzyme level in the ICR was exceedingly higher in females than in males. The enzyme contents in ICR SMG increased in males following castration and decreased after androgen injection in both castrated males and normal females. On the contrary, the enzyme level in C3H/HeN was decreased by castration and increased by androgen injection. Therefore, these sex differences in ICR and C3H/HeN mice depend on androgen, though the direction of the response is opposite.

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“…The isoelectric point was found to be pH 9.2, using isoelectric focusing by the method of Vesterberg and Srenseen. 19 ) The molecular weight of the FBP was estimated to be 23,000 for both gel filtration and The procedures-for disc and SDS disc electrophoreses are given in the MATERIALS AND METHODS. A, disc electrophoresis; B, SDS disc electrophoresis; MG, methyl green as the marker.…”

Section: Homogeneity and Some Properties Of Fbpmentioning

confidence: 99%