Isoelectric focusing studies of aldehyde dehydrogenases, alcohol dehydrogenases and oxidases from mammalian anterior eye tissues

Holmes, Roger S.; Cheung, Brenda; VandeBerg, John L.

doi:10.1016/0305-0491(89)90081-3

Cited by 13 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ADH-C2 is also the major ADH in ocular tissues, and is predominantly localized in high activity in mammalian cornea [14,15], This enzyme is present throughout postnatal development in the mouse eye, and increases in activity towards the end of the first week, at the time of eye opening. Ocular ADH-C2 continues to increase in activity subsequent- Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast, the major stomach and ocular isozyme, ADH-C2, may perform a more specific role in the detoxification of peroxidic aldehydes, generated following ex posure to oxygen or ultraviolet light [14], whereas ADH-B2 is more widely distributed in mouse tissues [4,11] and may carry out housekeeping functions in medium to long chain alcohol and aldehyde metabolism [6], In summary, these studies describe the postnatal development of mouse isozymes extracted from the liver, kidneys, stomach and eye, and demonstrate tissue-specific pat terns in each case. Distinct metabolic roles have been proposed for the three classes of murine ADHs [6,15], and their appearances during development may reflect increasing metabolic competences in oxido-reductive reactions of natural alcohols and aldehydes in the body.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Postnatal Development of Mouse Alcohol Dehydrogenases: Agarose Isoelectric Focusing Analyses of the Liver, Kidney, Stomach and Ocular Isozymes

Rout

Holmes²

1991

Neonatology

Self Cite

View full text Add to dashboard Cite

The postnatal development of alcohol dehydrogenase (ADH) isozymes was studied in liver, kidney, stomach and eye tissues of C57BL/6J inbred male mice using agarose isoelectric focusing and histochemical methods. Development profiles were tissue specific, with adult patterns being attained by 30 days in the liver and stomach, and by 42 days in the kidneys. Ocular ADH-C₂ increased in activity at the end of the first week, corresponding to the time of eye opening in the neonate.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Postnatal Development of Mouse Alcohol Dehydrogenases: Agarose Isoelectric Focusing Analyses of the Liver, Kidney, Stomach and Ocular Isozymes

Rout

Holmes²

1991

Neonatology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Indeed activities for these enzymes have been measured in some mammalian species previously and their potential significance for cataract has been discussed (Crabbe et al 1982;Harding and Crabbe 1984;Rao and Cotlier 1984;Holmes et al 1989). However the relatively high abundances of these enzymes even in some mammalian lenses, in which they do not serve as crystallins, suggests that they may belong to a group of moderately abundant enzymes perhaps with important functional or developmental roles in the lens.…”

Section: Functions and Origins Of Crystallinsmentioning

confidence: 96%

Lens protein expression in mammals: Taxon-specificity and the recruitment of crystallins

1991

View full text Add to dashboard Cite

Vertebrate lenses show remarkably taxon-specific patterns of protein composition, most obviously in the recruitment of enzymes as major crystallins. Phylogenetic relationships are particularly apparent in mammals. Here we describe eta-crystallin, which is probably identical to cytosolic aldehyde dehydrogenase, lens-specifically expressed at high abundance in the elephant shrews, primitive eutherians of the family Macroscelidae, and mu-crystallin, a novel lens protein expressed in some marsupials. We have also observed that enzymes that have been recruited as crystallins in some species are also moderately abundant in the lenses of other species. This hints that the origins of enzyme-crystallins may lie in a pool of enzymes widely expressed in lenses at fairly high levels, perhaps because they have important developmental or functional roles in the tissue.

show abstract

“…Homogenate supernatants were subjected to agarose-IEF and histochemically stained for ALDH and ADH activities according to previously described pro cedures [16]. Benzaldehyde (10 miff) and trans-2-hcxen-l-ol (10 mTf) were routinely used as substrates to stain ALDH and ADH activities, respectively.…”

Section: Ief and Stainingmentioning

confidence: 99%

Development of Aldehyde Dehydrogenase and Alcohol Dehydrogenase in Mouse Eye: Evidence for Light-Induced Changes

Downes

Holmes²

1992

Neonatology

View full text Add to dashboard Cite

We have studied the development of ocular aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH) activities in C57BL/6J inbred male mice. Eyes were removed from freshly killed mice, enucleated, extracted, and analyzed for enzyme activities for animals of various ages during neonatal development, up to the adult stage. Activity levels were compared between mice maintained from birth in either complete darkness or on a 12-hour light/dark cycle. Ocular ALDH activity increased dramatically ( > 30-fold) during the first 3 weeks of life. Moreover, light-adapted animals showed significantly higher ALDH activities from day 8. Ocular ADH activity also increased during development (> 5-fold) although the profile showed a steady increase to reach adult levels. Light-adapted mice showed no significant differences in ADH activity up to the weaning stage, as compared with mice maintained in darkness. These observations support proposals from earlier studies for major functional roles for both corneal ALDH and ADH in protecting the eye against ultraviolet light-induced damage.

show abstract

Isoelectric focusing studies of aldehyde dehydrogenases, alcohol dehydrogenases and oxidases from mammalian anterior eye tissues

Cited by 13 publications

References 23 publications

Postnatal Development of Mouse Alcohol Dehydrogenases: Agarose Isoelectric Focusing Analyses of the Liver, Kidney, Stomach and Ocular Isozymes

Postnatal Development of Mouse Alcohol Dehydrogenases: Agarose Isoelectric Focusing Analyses of the Liver, Kidney, Stomach and Ocular Isozymes

Lens protein expression in mammals: Taxon-specificity and the recruitment of crystallins

Development of Aldehyde Dehydrogenase and Alcohol Dehydrogenase in Mouse Eye: Evidence for Light-Induced Changes

Contact Info

Product

Resources

About