Isoelectric focusing of neutrophil alkaline phosphatase in trisomy 21 pregnancies

A, Brisson-Lougarre; Vergnes, Hugues; Grozdea, J.; Bourrouillou, G.; Colombiès, P

doi:10.1016/0009-8981(91)90241-4

Cited by 2 publications

(5 citation statements)

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“…That relatively small difference might be due to either a true trend or to the small population size and the large personal variations. These results differ from the markedly increased levels of maternal NAP previously reported in trisomy 21 pregnancies Vegnes et al, 1988;Brisson-Lougarre et al, 1991;Denier et al, 1996) but agree with the study of Aitken et al (1996).…”

Section: Discussionsupporting

confidence: 63%

“…Some previous studies confirmed, in cases of affected fetuses, a placental-like NAP activity distinct from that of control pregnant mothers. A greater proportion of activity along with an altered iso-electric focusing pattern and anomalous responses to some specific inhibitors were demonstrated in trisomy 21 affected pregnancies (Grozdea et al, 1983Vergnes et al, 1988;Brisson-Lougarre et al, 1991;Denier et al, 1996). In other reports no meaningful differences were elucidated (Aitken et al, 1996).…”

Section: Discussionmentioning

confidence: 91%

“…PAP is synthesized in the syncytiotrophoblast and reaches the maternal circulation early in the first trimester (Okamoto et al, 1990). An association between elevated activity of neutrophil PAP and trisomy 21 was reported in affected individuals (Lennox et al, 1962;Tangheroni et al, 1971;Grozdea et al, 1991), as well as in mothers with presently ongoing or previous Down syndrome pregnancies (Grozdea et al, 1983;Vergnes et al, 1988;Grozdea et al, 1988;Brisson-Lougarre et al, 1991;Denier et al, 1996). An increase in PAP activity was also described in maternal serum in the second trimester of affected pregnancies (Ind et al, 1994;Denier et al, 1996).…”

Section: Introductionmentioning

confidence: 89%

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Heat stable and urea resistant alkaline phosphatase in maternal neutrophils from normal and Down syndrome pregnancies

et al. 1999

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Activity levels of total and placental alkaline phosphatase (AP) were determined in maternal serum and neutrophils of normal and Down syndrome pregnancies. The placental iso‐enzyme (PAP) was identified by its relative stability to urea and heat. Significant increase in the activity of all iso‐enzymes across gestational stages was observed in maternal sera of 28 normal pregnancies. However, in the neutrophil extracts of the same blood samples no differences were detected between trimesters. Another set of experiments was aimed at finding diagnostic differences of PAP activity in maternal neutrophils of normal and trisomy 21 affected pregnancies. No differences of heat stable AP activity were found in maternal samples of 19 normal and 19 Down syndrome affected pregnancies. Urea resistant AP proportions were also similar when measured after 40 minutes of exposure (13 samples in each group). However, a marginally significant increase was observed in the mean value of the Down syndrome affected samples, after 60 minutes of urea treatment. In view of the above results we conclude that neutrophil AP activity is not as yet a useful marker for the screening of trisomy 21 fetuses. Copyright © 1999 John Wiley & Sons, Ltd.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 89%

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Heat stable and urea resistant alkaline phosphatase in maternal neutrophils from normal and Down syndrome pregnancies

et al. 1999

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“…In trisomy 21 pregnancies, NAP is thermostable at 56 and 65°C, significantly resis tant to 3.5 M urea treatment and insensitive to L-homoarginine, EDTA, and L-phenylalanine [17,18]. Isoelectric focusing clearly confirms that the NAP pattern in women with trisomy 21 pregnancies is different from normal NAP, suggesting the presence of more than one AP isoen zyme: a thermolabile and a thermostable one [9].…”

Section: Discussionsupporting

confidence: 50%

“…Circulat ing blood PMNs from pediatric carriers of an extra chro mosome 21 express an early placental variant of AP [6], Interestingly, our previous studies showed, in pregnant women with trisomy 21 fetuses, a significantly lower con centration of AP in PMN, a higher residual enzyme activ ity after heating or urea treatment, alteration of the bio chemical properties and the existence of a distinct AP isoenzyme pattern using the isoelectric focusing tech nique [7][8][9]. The present study is a continuation of our investigation on NAP by immunobiochemical, cytochemical and immunocytochemical procedures in pregnant women (17-22 weeks) with trisomy 21 or normal fetuses.…”

Section: Introductionmentioning

confidence: 99%

Changes in Immunological Properties of Neutrophil Alkaline Phosphatase in Trisomy 21 Pregnancies

Grozdea

Vergnes

et al. 1994

Acta Haematol

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Immunoreactivity, cytochemical, immunocytochemical characteristics and subcellular distribution of neutrophil alkaline phosphatase (NAP) were investigated in blood and/or smear samples from 18 women aged 23-46 years (mean 32.5 years) with trisomy 21 fetuses (17-21 weeks) and 28 women aged 20-42 years (mean 31 years) with normal fetuses (17-22 weeks). Immunochemical NAP investigations were carried out in 8 pathological and 8 normal pregnancies; cytochemical and immunocytochemical procedures were carried out in 18 pregnant women with trisomy 21 fetuses and 28 controls. NAP from women with trisomy 21 fetuses is characterized by: (1) a significant decrease in reactivity with anti-liver-type alkaline phosphatase (AP) and anti-NAP antisera;(2)low or very slight reactivity with antiplacental or anti-intestinal antibodies;(3)marked dispersion of NAP lead citrate reaction products or anti-NAP antibody colloidal gold-labelling in neutrophil cytoplasms, as detected by electron microscopy. This subcellular AP distribution (extramembranous) is different from that of normal NAP sites associated with plasma membrane, nuclear membrane and secretory vesicles. The NAP immunochemical and cytochemical characteristics suggest that neutrophils of a woman with a trisomy 21 fetus contain two AP isoenzymes: the liver/bone type and an atypical AP.

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Isoelectric focusing of neutrophil alkaline phosphatase in trisomy 21 pregnancies

Cited by 2 publications

References 5 publications

Heat stable and urea resistant alkaline phosphatase in maternal neutrophils from normal and Down syndrome pregnancies

Heat stable and urea resistant alkaline phosphatase in maternal neutrophils from normal and Down syndrome pregnancies

Changes in Immunological Properties of Neutrophil Alkaline Phosphatase in Trisomy 21 Pregnancies

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