Isochlorogenic acid C prevents enterovirus 71 infection via modulating redox homeostasis of glutathione

Cao, Zhipeng; Ding, Yue; Cao, Liang; Ding, Gang; Wang, Zhenzhong; Xiao, Wei

doi:10.1038/s41598-017-16446-7

Cited by 18 publications

(15 citation statements)

References 66 publications

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“…Strikingly, studies had shown that isochlorogenic acid B had a protective effect on liver fibrosis in mice with nonalcoholic steatohepatitis. And isochlorogenic acid C was found to prevent enterovirus 71 infection by regulating the redox homeostasis of glutathione [ 39 ]. Glycyrrhizic acid and protocatechualdehyde had been proven to have powerful antioxidant effects, which are considered to be promising potential human antiaging compounds [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea

Lin

Zhang

Zhuang

et al. 2020

Oxidative Medicine and Cellular Longevity

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Searching for natural and safe herbal tea with health benefits has attracted more and more attention, which is of great significance for reducing disease risk. A Chinese traditional herbal tea (HT) is rich in active ingredients extracted from natural plants. Numerous pharmacological studies showed that HT had the potential to improve health, including antidepression and antioxidant effects. In this study, we proposed a strategy to explore the role and underlying mechanism of HT in improving healthspan of a Caenorhabditis elegans model. First, we found that HT significantly prolonged the lifespan without reducing fertility in worms. Second, stress resistance (oxidative stress and heat stress) was enhanced and Aβ- and polyQ-induced toxicity was relieved significantly by HT treatment. Both fat deposition and age pigment accumulation were found to be significantly reduced in HT-treated worms. The locomotion in mid-late stages was improved, indicating that behavioral mobility was also significantly enhanced. Furthermore, the main components of HT were eighteen polyphenols and two terpenoids. Finally, it was found that this protective mechanism was positively correlated with the insulin/insulin-like growth factor signaling- (IIS-) dependent manner, which went through promoting the nuclear localization of DAF-16 and its downstream SOD-3 expression. These results suggested that HT had an important role in improving health, which might serve as a promising healthy tea.

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Section: Resultsmentioning

confidence: 99%

Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea

Lin

Zhang

Zhuang

et al. 2020

Oxidative Medicine and Cellular Longevity

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show abstract

“…Cells contain glutathione in both reduced (GSH) and oxidised (GSSG) forms, whose balance is crucial for maintaining cellular redox potential 9 . Disruption of the GSH/GSSG balance is associated with several enterovirus infections, including EV-A71 10 , CV-A16 and CV-B3 11–13 . Furthermore, depletion of GSH, using l -butathione sulfoximate (BSO) 14,15 and TP219 16 , blocks the assembly of protomeric units into pentamers 14,16,17 .…”

Section: Introductionmentioning

confidence: 99%

Glutathione facilitates enterovirus assembly by binding at a druggable pocket

et al. 2020

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Enteroviruses cause a range of human and animal diseases, some life-threatening, but there remain no licenced anti-enterovirus drugs. However, a benzene-sulfonamide derivative and related compounds have been shown recently to block infection of a range of enteroviruses by binding the capsid at a positively-charged surface depression conserved across many enteroviruses. It has also been established that glutathione is essential for the assembly of many enteroviruses, interacting with the capsid proteins to facilitate the formation of the pentameric assembly intermediate, although the mechanism is unknown. Here we show, by high resolution structure analyses of enterovirus F3, that reduced glutathione binds to the same interprotomer pocket as the benzene-sulfonamide derivative. Bound glutathione makes strong interactions with adjacent protomers, thereby explaining the underlying biological role of this druggable binding pocket and delineating the pharmacophore for potential antivirals.

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“…EV71 infection is strongly associated with severe HFMD, which is accompanied by neurological symptoms and usually leads to death. Therefore, many studies have focused on the identification of substances with activity against EV71, and these studies have identified many candidate drugs for the treatment of EV71 infection, such as amphotericin B, isochlorogenic acid C, acid beta‐glucosidase 1, sulfated rhamnan, and suramin . Considering their relatively low cytotoxicity, extracts from traditional Chinese medicines or foods show particular advantages and have attracted increasing attention …”

Section: Discussionmentioning

confidence: 99%

Virucidal activity and the antiviral mechanism of acidic polysaccharides against Enterovirus 71 infectionin vitro

Cui

Liu

et al. 2020

Microbiology and Immunology

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Enterovirus 71 (EV71) is the predominant pathogen for severe hand, foot, and mouth disease (HFMD) in children younger than 5 years, and currently no effective drugs are available for EV71. Thus, there is an urgent need to develop new drugs for the control of EV71 infection. In this study, LJ04 was extracted from Laminaria japonica using diethylaminoethyl cellulose‐52 with 0.4 mol/l NaCl as the eluent, and its virucidal activity was evaluated based on its cytopathic effects on a microplate. LJ04 is composed of fucose, galactose, and mannose and mainly showed good virucidal activity against EV71. The antiviral mechanisms of LJ04 were the direct inactivation of the virus, the blockage of virus binding, disruptions to viral entry, and weak inhibitory activity against the nonstructural protein 3C. The two most important findings from this study were that LJ04 inhibited EV71 proliferation in HM1900 cells, which are a human microglia cell line, and that LJ04 can directly inactivate EV71 within 2 hr at 37°C. This study demonstrates for the first time the ability of a polysaccharide from L. japonica to inhibit viral and 3C activity; importantly, the inhibition of 3C might have a minor effect on the antiviral effect of LJ04. Consequently, our results identify LJ04 as a potential drug candidate for the control of severe EV71 infection in clinical settings.

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Isochlorogenic acid C prevents enterovirus 71 infection via modulating redox homeostasis of glutathione

Cited by 18 publications

References 66 publications

Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea

Healthspan Improvements in Caenorhabditis elegans with Traditional Chinese Herbal Tea

Glutathione facilitates enterovirus assembly by binding at a druggable pocket

Virucidal activity and the antiviral mechanism of acidic polysaccharides against Enterovirus 71 infectionin vitro

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