Isobutanol production by a recombinant biocatalyst Shimwellia blattae (p424IbPSO): Study of the operational conditions

Acedos, Miguel G.; Ramon, Alberto; Morena, Susana de la; Santos, Victoria E.; Garcı́a-Ochoa, Félix

doi:10.1016/j.bej.2018.02.002

Cited by 11 publications

(28 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous work, S. blattae (p424IbPSO) strain has been used as an isobutanol producer. In both studies, the runs were conducted in sealed flasks in order to prevent the evaporation of isobutanol.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the bioprocess was carried out under limiting oxygen conditions, which are not the best conditions for bacterial growth and isobutanol production according to previous results . In the second study, the air chamber into the sealed bottle was increased in different experiments, increasing the available oxygen. It has been shown that the greater the amount of air inside closed bottles, the higher the production of isobutanol; thus, greater amounts of available oxygen favored the production of isobutanol …”

Section: Introductionmentioning

confidence: 99%

“…In the second study, the air chamber into the sealed bottle was increased in different experiments, increasing the available oxygen. It has been shown that the greater the amount of air inside closed bottles, the higher the production of isobutanol; thus, greater amounts of available oxygen favored the production of isobutanol …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Carbon flux distribution in the metabolism of Shimwellia blattae (p424IbPSO) for isobutanol production from glucose as function of oxygen availability

Acedos

Yustos

Santos

et al. 2018

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

BACKGROUND: The carbon flux distribution in Shimwellia blattae (p424IbPSO) cultures when glucose is employed as carbon source is studied in a stirred tank bioreactor changing the oxygen availability. The strain has been constructed to produce isobutanol combining the branched-chain amino acids pathway and Ehrlich pathway. The information of many similar genetic modified strains for this bioprocess in the literature presents significant discrepancies on the oxygen influence. RESULTS:The study carried out in this work pointed out the presence of other metabolites formed under aerobic conditions, such as isobutyric acid, 2,3-butanedione, acetoin, 2,3-butanediol and 3-oxo-1-buten-2-yl ether, not previously reported in the literature on the production of isobutanol with S. blattae (p424IbPSO) strain. CONCLUSION: In this work the routes or the chemical reactions involved in the bioprocess as a function of the availability of oxygen are elucidated. Anaerobic conditions are the best for isobutanol production from glucose employing this strain; around 12 g L −1 of isobutanol are obtained under these conditions. Aerobic conditions are not adequate to produce isobutanol, although the need for oxygen, at least for growth, is also demonstrated. Experimental procedure and set-upExperiments were carried out in a stirred tank bioreactor (STBR), BIOSTAT B-PLIS (Sartorius AG, Göttingen, Germany). The above described culture medium M9-2x was employed in all runs.The initial temperature of culture was 37 ∘ C, but after 2 h of growth the temperature was changed to 30 ∘ C and 1 mmol L -1 Isopropyl--D-1-thiogalactopyranoside (IPTG) was added to induce the expression of genes encoding the isobutanol synthetic pathway. The initial pH was fixed at 6.8, decreasing with time during growth; when pH reached a value of 6.0, it was controlled by the addition of 2 mol L -1 NaOH. 39 The airflow rate was set at 1 L L −1 min −1 from the beginning of the run and the stirrer speed was varied from 150 to 600 rpm in the different runs performed in aerobic conditions in order to change the oxygen transfer rate in J Chem Technol Biotechnol 2019; 94: 850-858 Analysis by GC-mass spectroscopyThis analysis was performed to determine the structures of the new compounds present in the S2 sample in comparison with the S1 sample. All the peaks appearing in the GC-MS chromatograms for both samples, S1 and S2, were identified. Table 3 gives the J Chem Technol Biotechnol 2019; 94: 850-858

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Carbon flux distribution in the metabolism of Shimwellia blattae (p424IbPSO) for isobutanol production from glucose as function of oxygen availability

Acedos

Yustos

Santos

et al. 2018

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

show abstract

“…Baez et al reported a concentration of 50 g L −1 of isobutanol obtained in fed‐batch operation, removing the product, under aerobic conditions, during 72 h. It is well know that isobutanol is a toxic compound for isobutanol producer strains, in this sense S. blattae (p424IbPSO) is a good candidate for this purpose because it has been reported that this recombinant bacteria is a robust strain and good isobutanol producer, about 5.7 g L −1 under oxygen deprivation conditions . In a previous work, the influences of several operating conditions, such as media composition, oxygen availability and pH, in growing cells of S. blattae cultures have been studied obtaining about 11 g L −1 of isobutanol and 69% attainment of theoretical maximum yield …”

Section: Introductionmentioning

confidence: 99%

“…In a previous work, the influences of several operating conditions, such as media composition, oxygen availability and pH, in growing cells of S. blattae cultures have been studied obtaining about 11 g L −1 of isobutanol and 69% attainment of theoretical maximum yield. 31 In resting cell operations, cells cannot grow but they are metabolically active; this operation type can have significant benefits compared to growing-cells cultures. [32][33][34][35] Better yields and flexible operation can be reached when working in resting cells, also increasing the culture stability, shift in metabolism toward isobutanol, thus enhancing the selectivity to isobutanol, and enhancing culture robustness.…”

Section: Introductionmentioning

confidence: 99%

Resting cells isobutanol production by Shimwellia blattae (p424IbPSO): Influence of growth culture conditions

Acedos

Santos

Garcı́a-Ochoa

2018

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

Isobutanol is a promising gasoline additive and could even be a potential substitute used directly as combustible. In this work, the production of isobutanol from glucose by Shimwellia blattae (p424IbPSO) in resting cell cultures is studied. This production has two stages, involving a resting cell phase that has not been studied before. The cell growth was carried out under different operating conditions: temperature and medium composition (YE, ammonium, and IPTG concentrations), looking for the highest isobutanol production. Moreover, the cells were collected at three different growth times checking their isobutanol production capacity. The best operating conditions have been determined as: 30°C of temperature, a medium containing 1.5 g L−1 YE and 1.4 g L−1of ammonium as nitrogen sources, adding 0.5 mM IPTG as inducer. The cells collected at early growth times are significantly more active. The use of S. blattae (p424IbPSO) in resting cells is a good strategy for the production of isobutanol from glucose yielding better results than in batch growth cultures, a yield of 60% attainment of theoretical maximum yield is obtained under optimal conditions. In addition, it has been demonstrated that if the cells are cultured at higher temperatures and with high IPTG concentrations, inclusion bodies are formed in the cytoplasm inhibiting the isobutanol production in the resting cell stage.

show abstract

Utilisation/upgrading of orange peel waste from a biological biorefinery perspective

Torre

Martín-Domínguez

Acedos

et al. 2019

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Isobutanol production by a recombinant biocatalyst Shimwellia blattae (p424IbPSO): Study of the operational conditions

Cited by 11 publications

References 22 publications

Carbon flux distribution in the metabolism of Shimwellia blattae (p424IbPSO) for isobutanol production from glucose as function of oxygen availability

Carbon flux distribution in the metabolism of Shimwellia blattae (p424IbPSO) for isobutanol production from glucose as function of oxygen availability

Resting cells isobutanol production by Shimwellia blattae (p424IbPSO): Influence of growth culture conditions

Utilisation/upgrading of orange peel waste from a biological biorefinery perspective

Contact Info

Product

Resources

About