Isobavachalcone ameliorates diabetic nephropathy in rats by inhibiting the NF‐κB pathway

Abstract

Isobavachalcone (ISO) exhibits good anti‐inflammatory activity. We evaluated the renoprotective effects of ISO against diabetic nephropathy (DN). Diabetic rats established by the single injection of streptozotocin (STZ) were orally treated with ISO. The levels of serum creatinine (Scr), blood urea nitrogen (BUN), and 24 hr urinary protein were measured. In this study, ISO effectively ameliorated renal damage by reducing BUN, Scr, and 24 hr urinary protein and also improved kidney pathological appearances. ISO prevented STZ‐caused apoptosis in the glomerular tissue in vivo and blocked the high glucose (HG)‐induced growth inhibitory effect in human renal glomerular endothelial cells in vitro. Moreover, ISO reduced pro‐inflammatory mediator production and blocked the NF‐κB pathway in the damaged renal tissues and HG‐treated HRGEC cells. Taken together, the results of this study indicate that ISO consumption might have significant beneficial effects on the DN and this action might be correlated with the modulation of the NF‐κB pathway. Practical applications ISO is an active compound from the dried ripe fruit of Psoralea corylifolia L. seed, which is traditionally served as a food ingredient in Asia. In this investigation, we observed the beneficial effects of ISO on a murine model with DN. Further research revealed that the protective action of ISO might be connected with its weak hypoglycaemic and notable anti‐inflammatory effects. Our research data suggest that ISO‐enriched food might be a good choice for people suffering from DN.

Prognostic Value of Serum Interleukin-6, NF-κB plus MCP-1 Assay in Patients with Diabetic Nephropathy

“…NF-κB originating from inflammatory response activation stimulates the induction of IL-6 and MCP-1 secretion, which in turn activates IκB kinase complexes (IKKs). IKKs phosphorylate the serine of the IκB subunit regulatory site of the trimeric p50/p65/IκB, resulting in ubiquitination modification of the IκB subunit, the degradation by proteases, and subsequent release of p50/ p65 and nuclear translocation to the κB site on the corresponding target gene to regulate gene transcription [14][15][16][17]. IL-6 and MCP-1 can activate a large number of inflammatory cells, and the resulting inflammatory cascade amplification is associated with distant organ damages, which positively regulates the activation of NF-κB and aggravates the damage to the intracellular and basement membranes, thereby eliciting proteinuria.…”

Isobacachalcone induces autophagy and improves the outcome of immunogenic chemotherapy

“…In vitro, ISO reduces Aβ42 aggregation in SH-SY5Y cells 60 and the tumor necrosis factor-α (TNFα)-induced atrophy of C2C12 myotubes 61 . In rodents, ISO attenuates Parkinson's disease induced by the toxin 1-methyl-4phenyl-1,2,3,6-tetrahydropyridine (MPTP) 62 , sephadexinduced lung injury 63 , as well as streptozotocin-induced diabetic nephropathy 64 . This suggests that ISO has a wide range of cytoprotective effects that might be explained by its autophagy-inducing activity.…”

Nucleoporin 160 (NUP160) inhibition alleviates diabetic nephropathy by activating autophagy

“…N = 8 mice each group. To establish a mouse model of DN, 60 mg/kg streptozotocin (STZ; Sigma-Aldrich, USA) in 0.01 M citrate buffer was intraperitoneally injected into the mice for 5 consecutive days [ 26 ]. Mice in the sham group were injected with the same amount of citrate buffer.…”