Islet-1 marks the early heart rudiments and is asymmetrically expressed during early rotation of the foregut in the chick embryo

Yuan, Shipeng; Schoenwolf, Gary C.

doi:10.1002/1097-0185(20001001)260:2<204::aid-ar90>3.0.co;2-5

Cited by 86 publications

(73 citation statements)

References 25 publications

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“…We found MMP-2 was specifically expressed in the splanchnic mesoderm adjacent to the ventral floor of the foregut, i.e., in the emerging anterior, or so-called secondary, heart field of stage 9ϩ embryos where cells are migrating within the aortic sac region and contribute cells to the right ventricle and outflow tract of the heart. This pattern of MMP-2 expression parallels that described for Islet-1-expressing cells that define the anterior (secondary) heart region (Cai et al, 2000;Yuan and Schoenwolf, 2000;Cai et al, 2003). TIMPs inhibit MMP activity, but some, like TIMP-2, can also facilitate proMMP-2 activation at the cell surface, depending on its concentration (Gomez et al, 1997).…”

Section: Mmp-2 and Timp-2 Expression During Early Cardiac Morphogenesissupporting

confidence: 68%

Cardiac morphogenesis: Matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping

Linask

Han

Cai

et al. 2005

Developmental Dynamics

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During heart organogenesis, the spatiotemporal organization of the extracellular matrix (ECM) undergoes significant remodeling. Because matrix metalloproteinases (MMPs) are known to be key regulators of cell-matrix interactions, we analyzed the role(s) of MMPs, and specifically MMP-2, in early heart development. Both MMP-2 neutralizing antibody and the broad-spectrum MMP inhibitor Ilomastat in a temporal manner, when applied between chick embryonic stages 5 (primitive streak stage) to stage 12 (ϳ16-somites), produced severe heart tube defects. Exposure to the MMP inhibitor at stage 5 produced various degrees of cardia bifida. At the seven-somite stage, MMP-2/Ilomastat inhibition caused a shift in normal left-right patterning of cell proliferation within the dorsal mesocardium and mesoderm of the anterior heart field that correlated with a change in looping direction. MMP inhibition at the 10-to 12-somite stage resulted in an arrest of heart tube bending by inhibiting the breakdown of the dorsal mesocardial ECM. The experimental observations suggest that MMP activity regulates the coordination of early heart organogenesis by affecting ventral closure of the heart and gut tubes, asymmetric cell proliferation in the dorsal mesocardium to drive looping direction, and ECM degradation within the dorsal mesocardium allowing looping to proceed toward completion. Developmental Dynamics 233:739 -753, 2005.

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Section: Mmp-2 and Timp-2 Expression During Early Cardiac Morphogenesissupporting

confidence: 68%

Cardiac morphogenesis: Matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping

Linask

Han

Cai

et al. 2005

Developmental Dynamics

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“…Isl1 has previously been reported to be expressed in the adult rat thyroid gland, although only C-cells showed Isl1 immunoreactivity in this study (Thor et al, 1991). By in situ hybridization, Isl1 was observed in the early thyroid placode in chicken (Yuan and Schoenwolf, 2000). However, whether this is imprinted by a general Isl1 expression in the anterior endoderm or thyroid progenitors specifically express Isl1 during the developmental process is not known.…”

Section: Introductionmentioning

confidence: 53%

Expression of Islet1 in thyroid development related to budding, migration, and fusion of primordia

Westerlund¹,

Andersson²,

Carlsson³

et al. 2008

Developmental Dynamics

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The LIM homeodomain transcription factor Isl1 was investigated in mouse thyroid organogenesis. All progenitor cells of the midline thyroid diverticulum and lateral primordia (ultimobranchial bodies) expressed Isl1. This pattern persisted until the growing anlagen fused at embryonic day (E) 13.5. In Isl1 null mutants thyroid progenitors expressing Nkx2.1 and Pax8 were readily specified in the anterior endoderm but the size of the thyroid rudiment was reduced. In late development, only immature C-cells expressed Isl1. In the adult gland the number of Isl1؉ cells was small compared with cells expressing calcitonin. Analysis of microarray profiles indicated a higher level of Isl1 expression in medullary thyroid carcinomas than in tumors derived from follicular cells. Together, these findings suggest that Isl1 may be a novel regulator of thyroid development before terminal differentiation of the endocrine cell types. Isl1 is an embryonic C-cell precursor marker that may be relevant also in cancer developed from the mature C-cell. Developmental Dynamics 237:3820 -3829, 2008.

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“…Apparently, Ednra-lacZ/EGFP-positive cells are distinct from this population because they are localized to the ventral region of the inflow and are Isl1-negative at the crescent/tube-forming stages. However, Ednra-lacZ/EGFP-positive cells might also be derived from the Isl1-positive pool as Isl1 is initially expressed in all cardiogenic mesoderm and is downregulated on differentiation (Prall et al, 2007;Yuan and Schoenwolf, 2000). At later stages, many atrial cells express Ednra-lacZ/EGFP, suggesting that the second heart field-derived cells may also start to express this gene at a later time than do first heart field-derived cells.…”

Section: Discussionmentioning

confidence: 99%

Endothelin receptor type A expression defines a distinct cardiac subdomain within the heart field and is later implicated in chamber myocardium formation

et al. 2010

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SUMMARYThe avian and mammalian heart originates from two distinct embryonic regions: an early differentiating first heart field and a dorsomedially located second heart field. It remains largely unknown when and how these subdivisions of the heart field divide into regions with different fates. Here, we identify in the mouse a subpopulation of the first (crescent-forming) field marked by endothelin receptor type A (Ednra) gene expression, which contributes to chamber myocardium through a unique type of cell behavior. Ednra-lacZ/EGFP-expressing cells arise in the ventrocaudal inflow region of the early linear heart tube, converge to the midline, move anteriorly along the outer curvature and give rise to chamber myocardium mainly of the left ventricle and both atria. This movement was confirmed by fluorescent dye-labeling and transplantation experiments. The Ednra-lacZ/EGFPexpressing subpopulation is characterized by the presence of Tbx5-expressing cells. Ednra-null embryonic hearts often demonstrate hypoplasia of the ventricular wall, low mitotic activity and decreased Tbx5 expression with reciprocal expansion of Tbx2 expression. Conversely, endothelin 1 stimulates ERK phosphorylation and Tbx5 expression in the early embryonic heart. These results indicate that early Ednra expression defines a subdomain of the first heart field contributing to chamber formation, in which endothelin 1/Ednra signaling is involved. The present finding provides an insight into how subpopulations within the crescent-forming (first) heart field contribute to the coordination of heart morphogenesis through spatiotemporally defined cell movements.

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Islet-1 marks the early heart rudiments and is asymmetrically expressed during early rotation of the foregut in the chick embryo

Cited by 86 publications

References 25 publications

Cardiac morphogenesis: Matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping

Cardiac morphogenesis: Matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping

Expression of Islet1 in thyroid development related to budding, migration, and fusion of primordia

Endothelin receptor type A expression defines a distinct cardiac subdomain within the heart field and is later implicated in chamber myocardium formation

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