Island Cotton Gbve1 Gene Encoding A Receptor-Like Protein Confers Resistance to Both Defoliating and Non-Defoliating Isolates of Verticillium dahliae

Zhang, Baolong; Yang, Youxin; Chen, Tianzi; Yu, Wenbao; Liu, Tingli; Li, Hongjuan; Fan, Xiaohui; Ren, Yongzhe; Shen, Danyu; Liu, Li; Dou, Daolong; YouHong, Chang

doi:10.1371/journal.pone.0051091

Cited by 104 publications

(126 citation statements)

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“…All monocots, gymnosperms, and ferns, however, are immune to the disease. So far, there is no effective fungicide against Verticillium wilt, and comprehensive prevention and control measures are limited solely to planting-resistant varieties (Jian et al 2003;Zhang et al 2012a). Verticillium wilt-resistant resources mainly exist in the germplasms of Asiatic and sea island cotton, which are difficult to use directly because of crossing barriers and limited backcross choices.…”

Section: Introductionmentioning

confidence: 99%

“…Ve1 is the first Verticillium wilt-resistant gene to be reported. Some homologous genes, including SlVe1 from Solanum licopersicoides (Chai et al 2003), StVe from Solanum torvum Swartz, mVe1 from Mentha longifolia (L.) Huds (Vining and Davis 2009), GbVe , and GbVe1 (Zhang et al 2012a) from island cotton, have been verified via their resistant characters. All these genes encode leucine-rich-repeat receptorlike proteins (eLRR-RLPs), which differ from receptor-like kinases in that they lack a cytoplasmic kinase domain and carry only a short cytoplasmic tail that lacks obvious signaling motifs.…”

Section: Introductionmentioning

confidence: 99%

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A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton

et al. 2014

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Leucine-rich-repeat receptor-like proteins (eLRRRLPs) play significant roles in plant defense against pathogens and in plant development. Several eLRR-RLP genes such as Ve1, Gbve, and Gbve1 have been reported to confer resistance to Verticillium dahliae. Gbvdr5, a newly discovered RLP gene from V. dahliae-resistant island cotton cultivar H7124, has a short tail as Ve1. There is a cytosine deletion in Gbvdr5 homologous genes at nucleotide position 2765, which is downstream from the initiation codon in all susceptible upland cotton cultivars analyzed. This deletion was found to cause premature termination of the protein, creating a 937 aa product, but the Gbvdr5 protein had the full 1,077 aa. Transient expression analyses indicated that Gbvdr5 is localized on the plasma membrane. Quantitative polymerase chain reaction analysis revealed that the Gbvdr5 gene was activated by SA, MeJA, ABA, and ETH, and it was induced by V. dahliae isolates V991 and DF-CQ-2 in H7124, whereas was unchanged or repressed in susceptible upland cotton Simian 3. Gbvdr5-promoter-driven GUS activity was found mostly in the root tips and stem growing points of transgenic Arabidopsis. Silencing of Gbvdr5 in Verticillium-wilt-resistant cotton H7124 compromised cotton resistance to V. dahliae isolates V991 and BP2. The resistance was verified by transforming the Gbvdr5 gene into Arabidopsis and upland cotton through Agrobacterium-mediated transformation. Overexpression of the Gbvdr5 gene endowed transgenic Arabidopsis with resistance to defoliating isolate V991 and non-defoliating isolate BP2, but it had no effect on either DF-CQ-2 or JR2 of V. dahliae. The transformed cotton also had confirmed resistance to V991 and BP2. More callose deposition, more expression of the defense-related genes PR1 and PR5, and HR-mimic cell death were observed in the transgenic Arabidopsis when inoculated with V. dahliae. This demonstrated that Verticillium-plant interactions may involve some specific ways of recognizing V. dahliae and Gbvdr5 may be a suitable candidate gene for breeding Verticillium-wilt-resistant cotton lines.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton

et al. 2014

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“…Ve1 is the only R gene isolated using map-based cloning from tomato (Solanum lycopersicum) and has been shown to provide race-specific resistance to race 1 strains of V. dahliae and V. albo-atrum in tomato and Arabidopsis (17,29). Although several genes homologous to Ve1 have been cloned from cotton (30,31), it is unclear whether Ve1-mediated resistance signaling also exists in cotton.…”

mentioning

confidence: 99%

Proteomic and Virus-induced Gene Silencing (VIGS) Analyses Reveal That Gossypol, Brassinosteroids, and Jasmonic acid Contribute to the Resistance of Cotton to Verticillium dahliae

Gao

Long

Zhu

et al. 2013

Molecular & Cellular Proteomics

244

240

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Verticillium wilt causes massive annual losses of cotton yield, but the mechanism of cotton resistance to Verticillium dahliae is complex and poorly understood. In this study, a comparative proteomic analysis was performed in resistant cotton (Gossypium barbadense cv7124) on infection with V. dahliae. A total of 188 differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) analysis and could be classified into 17 biological processes based on Gene Ontology annotation. Most of these proteins were implicated in stimulus response, cellular processes and metabolic processes. Based on the proteomic analysis, several genes involved in secondary metabolism, reactive oxygen burst and phytohormone signaling pathways were identified for further physiological and molecular analysis. The roles of the corresponding genes were further characterized by employing virus-induced gene silencing (VIGS). Based on the results, we suggest that the production of gossypol is sufficient to affect the cotton resistance to V. dahliae. Silencing of GbCAD1, a key enzyme involving in gossypol biosynthesis, compromised cotton resistance to V. dahliae. Reactive oxygen species and salicylic acid signaling may be also implicated as regulators in cotton responsive to V. dahliae according to the analysis of GbSSI2, an important regulator in the crosstalk between salicylic acid and jasmonic acid signal pathways. Moreover, brassinosteroids and jasmonic acid signaling may play essential roles in the cotton disease resistance to V. dahliae. The brassinosteroids signaling was activated in cotton on inoculation with V. dahliae and the disease resistance of cotton was enhanced after exogenous application of brassinolide. Meanwhile, jasmonic acid signaling was also activated in cotton after inoculation with V. dahliae and brassinolide application. These data provide highlights in the molecular basis of cotton resistance to V. dahliae. Molecular & Cellular

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“…To measure the growth rate and sporulation in vitro, we were selected the Czapek Dox and PDA as basic medium (Zhang et al, 2012). The pH of the test media were maintained at 5.5 and which were optimal for the growth and sporulation in majority of fungi.…”

Section: Growth Rate and Sporulationmentioning

confidence: 99%

Trade-Offs between Growth Rate, Sporulation and Pathogenicity in Verticillium dahliae

El-Sharawy¹,

Hu²,

Yang³

2015

JAS

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Verticillium dahliae is a soil-borne phytopathogenic fungus that causes vascular wilt diseases in cotton (Gossypium hirsutum L.). We have illustrated the correlations between growth rate, sporulation and pathogenicity in V. dahliae mutants. A total of 196 V. dahliae mutants generated by Agrobacterium tumefaciens-mediated transformation, was used throughout this study. Compared with a control strain, the mutants could be significantly grouped into three types by differentiation with to that of control strain. The results indicated that linear regression analysis of the relationship was performed and the gradient and intercept were shown for growth rate and disease index. The correlation coefficient was observed if the infection is high. When their correlation of the growth rate is high, the growth rate and sporulation quantity of mutants had significantly a power correlation. This study, which may play an important role in the population structure of the pathogen, highlights fundamental biological differences between the mutants involving different infection strategies.

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Island Cotton Gbve1 Gene Encoding A Receptor-Like Protein Confers Resistance to Both Defoliating and Non-Defoliating Isolates of Verticillium dahliae

Cited by 104 publications

References 46 publications

A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton

A Cotton Gbvdr5 Gene Encoding a Leucine-Rich-Repeat Receptor-Like Protein Confers Resistance to Verticillium dahliae in Transgenic Arabidopsis and Upland Cotton

Proteomic and Virus-induced Gene Silencing (VIGS) Analyses Reveal That Gossypol, Brassinosteroids, and Jasmonic acid Contribute to the Resistance of Cotton to Verticillium dahliae

Trade-Offs between Growth Rate, Sporulation and Pathogenicity in Verticillium dahliae

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