Introduction: To evaluate the effect of bilirubin levels on auditory screening results in newborns. Methods: In total, 43 newborns (24 males and 19 females) who underwent the neonatal auditory screening in our hospital were included in the present study. All newborns were evaluated before they were discharged from the hospital. They were divided into two groups according to the results of the screening. Group A consisted of newborns that cleared the screening, whereas group B consisted of the remaining cases. Results: There were 31 newborns (16 males, 15 females) in group A and 12 newborns (8 males, 4 females) in group B. In group A, mean total serum bilirubin level was 24.7±8.1 mg/dl, mean serum indirect bilirubin level was 23.3±8.0 mg/dl and mean serum direct bilirubin level was 1.1±1.6 mg/dl. In group B, these values were 28.1±6.6 mg/dL, 27.1±6.4 mg/dL, and 1.4±1.3 mg/dL, respectively. There were no statistically significant between-group differences between total serum bilirubin levels, indirect, and direct serum bilirubin levels (p-values: 0.279, 0.168, and 0.233, respectively). There was no significant difference between hematocrit, reticulocyte, leukocyte, and platelet values of the groups (all p-values >0.05). Discussion and Conclusion: The results of the newborn auditory screening were not affected by serum bilirubin levels. However, the clinician should consider that the neonatal jaundice is a risk factor for hearing loss.