Ischemic brain injury: A consortium analysis of key factors involved in mesenchymal stem cell-mediated inflammatory reduction

McGuckin, Colin; Jurga, Marcin; Miller, Anne Marie; Sarnowska, Anna; Wiedner, Marc; Boyle, Noreen T.; Lynch, Marina A.; Jabłońska, Anna; Drela, Katarzyna; Łukomska, Barbara; Domanska-Janik, Krystyna; Kenner, Lukas; Moriggl, Richard; Degoul, Olivier; Perruisseau-Carrier, Claire; Forraz, Nico

doi:10.1016/j.abb.2013.02.005

Cited by 57 publications

(43 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, significantly increased levels of GFAP were noted in the striatum after cerebral I/R injury, and these levels then decreased with intravenous administration of HUMSCs. This finding agrees with the results of previous studies using other types of MSCs (16,18).…”

Section: Discussionsupporting

confidence: 83%

“…Because of the time needed to prepare autologous stem cells after an unexpected stroke, such cells can only be administered several weeks later, and previous studies have focused on post-acutephase intervention (16,17). Promising experimental animal data suggest that early stem cell administration can interrupt the initiation of the very beginning of the ischemic cascade (15).…”

Section: Introductionmentioning

confidence: 99%

“…However, these techniques raise the possibility of additional trauma from transplantation surgery and are often impractical for patients with clinically serious conditions. Recent studies have demonstrated that cell-based therapy is not intended to replace damaged cells but rather to remodel the central nervous system by promoting neuroplasticity, angiogenesis, and immunomodulation (15,16).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional recovery after acute intravenous administration of human umbilical cord mesenchymal stem cells in rats with cerebral ischemia-reperfusion injury

Zhang

Zhang³

et al. 2015

IRDR

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functional recovery after acute intravenous administration of human umbilical cord mesenchymal stem cells in rats with cerebral ischemia-reperfusion injury

Zhang

Zhang³

et al. 2015

IRDR

View full text Add to dashboard Cite

“…Recent experimental studies showed that MSC are more efficient at modulating the neural microenvironment by promoting growth factor release and glial activation, mainly of oligodendrocytes, which enables better structural and functional reorganization compared with hematopoietic stem cells used in this study. 96,97 This observation is supported by articles included in this review, which, besides confirming the ischemic region reduction after 7 days after stem cells' administration in both photothrombosis and temporary occlusion of the middle cerebral artery model, also showed an improvement in memory behavioral tests, such as the Morris water maze, and adapted neurological scales.…”

supporting

confidence: 52%

Therapeutics with SPION-labeled stem cells for the main diseases related to brain aging: a systematic review

Alvarim¹,

Nucci²,

Mamani³

et al. 2014

IJN

View full text Add to dashboard Cite

The increase in clinical trials assessing the efficacy of cell therapy for structural and functional regeneration of the nervous system in diseases related to the aging brain is well known. However, the results are inconclusive as to the best cell type to be used or the best methodology for the homing of these stem cells. This systematic review analyzed published data on SPION (superparamagnetic iron oxide nanoparticle)-labeled stem cells as a therapy for brain diseases, such as ischemic stroke, Parkinson’s disease, amyotrophic lateral sclerosis, and dementia. This review highlights the therapeutic role of stem cells in reversing the aging process and the pathophysiology of brain aging, as well as emphasizing nanotechnology as an important tool to monitor stem cell migration in affected regions of the brain.

show abstract

“…255 MSC cellular therapy has proven to be safe for a number of vascular disorders, such as coronary artery disease, peripheral vascular disease, erectile dysfunction, and stroke, and is an attractive option for patients who are poor surgical candidates. [256][257][258][259][260] Despite these successes, the problem remains that adult stem cells from elderly donors, the very people who most frequently require enhanced peripheral stem cell function for tissue repair, undergo changes in their functional capacity as a result of aging. 104 This decline in functional capacity, therefore therapeutic utility, has been combatted using some surprisingly simple interventions: conditioning with hypoxia prior to transplant, for example, has been extensively documented as effective for reducing reactive oxygen species production by adult stem cells and improving their therapeutic efficacy in many in vivo ischemia and other disease models.…”

Section: Therapeutic Targets and Clinical Trialsmentioning

confidence: 99%

Altering Fate Determination of Human Bone Marrow-Derived Mesenchymal Stem Cells through Environmental and Transcriptional Cues

Boyette

View full text Add to dashboard Cite

i Abstract Preservation of adult stem cells pools is critical for maintaining tissue homeostasis into old age. Exhaustion of adult stem cell pools as a result of deranged metabolic signaling and premature senescence as a response to oncogenic insults to the somatic genome both contribute to tissue degeneration with age. Both progeria, an extreme example of early-onset aging, and heritable longevity have provided avenues to study regulation of the aging program and its impact on adult stem cell compartments.Stem cells are promising candidate cells for regenerative applications because they possess high proliferative capacity and the potential to differentiate into other cell types.Mesenchymal stem cells (MSCs) are easily sourced but do not retain their proliferative and multi-lineage differentiative capabilities after prolonged ex vivo propagation. We investigated the use of hypoxia as a preconditioning agent and in differentiating cultures to enhance MSC function. Culture in 5% ambient O2 consistently enhanced clonogenic potential of primary MSCs from all donors tested. We determined that enhanced clonogenicity was attributable to increased proliferation, increased vascular endothelial growth factor (VEGF) secretion, and increased matrix turnover. Hypoxia did not impact the incidence of cell death. Application of hypoxia to osteogenic cultures resulted in enhanced total mineral deposition, although this effect was only detected in MSCs preconditioned in normoxic conditions. Osteogenesisassociated genes were up-regulated in hypoxia, and alkaline phosphatase activity was enhanced. Adipogenic differentiation was inhibited by exposure to hypoxia during differentiation. Chondrogenesis in 3-dimensional (3D) pellet cultures was inhibited by preconditioning with hypoxia. However, in cultures expanded under normoxia, hypoxia ii applied during subsequent pellet culture enhanced chondrogenesis. While hypoxic preconditioning appears to be an excellent way to expand a highly clonogenic progenitor pool, our findings suggest that it may blunt the differentiation potential of MSCs, compromising their utility for regenerative tissue engineering. Exposure to hypoxia during differentiation (post normoxic expansion), however, appears to result in a greater quantity of functional osteoblasts and chondrocytes and ultimately a larger quantity of high quality differentiated tissue.Media formulations for MSC culture and differentiation vary between labs; two notable variables addressed inconsistently in the field are ascorbate supplementation and glucose concentration in MSC expansion medium. Glucose and ascorbate effects are coupled to the Hif1α pathway. Therefore, we examined the effects of ascorbate supplementation and low versus high glucose medium in the settings of hypoxia and normoxia on colony formation, colony morphology, proliferation, VEGF secretion, and senescence of MSCs. We found that there is no one medium formulation that maximizes MSC performance for all of these assays.Depending on the desired outcome of the culture, t...

show abstract

Ischemic brain injury: A consortium analysis of key factors involved in mesenchymal stem cell-mediated inflammatory reduction

Cited by 57 publications

References 43 publications

Functional recovery after acute intravenous administration of human umbilical cord mesenchymal stem cells in rats with cerebral ischemia-reperfusion injury

Functional recovery after acute intravenous administration of human umbilical cord mesenchymal stem cells in rats with cerebral ischemia-reperfusion injury

Therapeutics with SPION-labeled stem cells for the main diseases related to brain aging: a systematic review

Altering Fate Determination of Human Bone Marrow-Derived Mesenchymal Stem Cells through Environmental and Transcriptional Cues

Contact Info

Product

Resources

About