Isatin inhibits SH-SY5Y neuroblastoma cell invasion and metastasis through MAO/HIF-1α/CXCR4 signaling

Sun, Wenyan; Zhang, Li; Hou, Lin; Cao, Ju; Zhao, Shengmin; Yaoyue, Wei,

doi:10.1097/cad.0000000000000505

Cited by 14 publications

(17 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identification of a representative group of isatin-binding proteins suggests that isatin acting on a large number of biological targets can exhibit many biological and potential pharmacological actions. Identification of some isatin responsive genes [22][23][24][25]48,96,97] and various nucleoproteins interacting with this regulator [49,50] opens a new direction in biomedical implications of this compound. Characterization of mechanisms responsible for implementation of isatin actions is especially important in the context of numerous isatin analogues, which are tested as potentially attracting pharmacological tools (e.g., [1][2][3][4][5]).…”

Section: Resultsmentioning

confidence: 99%

“…The inhibition of migration and invasion was observed already at 50 mM isatin and demonstrated further concentrationdependent decrease up to 200 lM isatin (higher concentrations were not studied). Incubation of cells with 200 lM isatin decreased cyclin D1, monoamine oxidase A, HIF-1a (hypoxiainducible factor 1-alpha), and CXCR4 (chemokine receptor type 4) proteins, which was demonstrated by Western blot analysis [25,48]. The decrease in expression of metalloproteinases MMP-2 and MMP-9 observed after incubation of SH-SY5Y cells with 100-400 mM was demonstrated at the mRNA and proteins levels.…”

Section: Isatin As An Antitumor Agentmentioning

confidence: 88%

“…Recent studies have shown that isatin inhibits not only proliferation of SH‐SY5Y cells but also their migration and invasion . The inhibition of migration and invasion was observed already at 50 µM isatin and demonstrated further concentration‐dependent decrease up to 200 μM isatin (higher concentrations were not studied).…”

Section: Isatin As An Antitumor Agentmentioning

confidence: 97%

“…This suggests that exogenously administered isatin is unevenly distributed in various target cells and therefore local concentrations of isatin in particular brain regions may be even higher. In the context of pharmacologically achievable concentrations, it should be noted that isatin concentrations exceeding (50-100) mM induce apoptosis in various cell cultures [20][21][22][23][24][25]…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isatin, an endogenous nonpeptide biofactor: A review of its molecular targets, mechanisms of actions, and their biomedical implications

et al. 2018

View full text Add to dashboard Cite

Isatin (indole-2,3-dione) is an oxidized indole. It is widely distributed in mammalian tissues and body fluids, where isatin concentrations vary significantly from <0.1 to > 10 µM. Isatin output is increased under conditions of stress. Exogenously administered isatin is characterized by low toxicity, mutagenicity, and genotoxicity in vivo. Cytotoxic effects of isatin on various cell cultures are usually observed at concentrations exceeding 100 µM. Binding of [ H]isatin to rat brain sections is consistent with its physiological concentrations. Proteomic analysis of mouse and rat brain isatin-binding proteins revealed about 90 individual proteins, which demonstrated significant interspecies differences (rat versus mouse). Certain evidence exist that redox state(s) and possibly other types of posttranslational modifications regulate affinity of target proteins to isatin. Recent data suggest that interacting with numerous intracellular isatin binding proteins, isatin can act as a regulator of complex protein networks in norm and pathology. Physiological concentrations of isatin in vitro inhibit monoamine oxidase B and natriuretic peptide receptor guanylate cyclase, higher (neuroprotective) concentrations (50-400 μM) cause apoptosis of various (including malignant tumor) cell lines and influence expression of certain apoptosis-related genes. Being administered in vivo, isatin exhibits various behavioral effects; it attenuates manifestations of MPTP-induced parkinsonism and tumor growth in experimental animal models. © 2017 BioFactors, 44(2):95-108, 2018.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Isatin As An Antitumor Agentmentioning

confidence: 88%

Section: Isatin As An Antitumor Agentmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isatin, an endogenous nonpeptide biofactor: A review of its molecular targets, mechanisms of actions, and their biomedical implications

et al. 2018

View full text Add to dashboard Cite

show abstract

“…When incubated with 200μmol/l isatin, the protein expression of H3K4m1 was significantly increased compared with that of control cells (Fig.3A, Fig.3B) indicating isatin may influence SH-SY5Y cell invasion and metastasis through inhibiting LSD1 activity. It's been reported that LSD1 may sustains carcinoma cell proliferation through the PI3K/AKT pathway [30]. But whether LSD1 decrease the expression of PTEN ,a typical inhibitor of PI3K/AKT pathway [31,32] , remains unclear in NB cells.…”

Section: The Expressions Of H3k4 M1 and Pten Are Increasedmentioning

confidence: 99%

Isatin Inhibits SH-SY5Y Neuroblastoma Cell Invasion and Metastasis through LSD1 Activity Inhibition

Cong¹,

Hou²,

Luo³

et al. 2019

ijSciences

Self Cite

View full text Add to dashboard Cite

Isatin has received much attention in recent years due to its anti-cancer properties [1], which offer important medical benefits. Isatin is an endogenous oxidized indole with a wide spectrum of behavioral and metabolic effects[2] and is commonly found in mammalian tissues and fluids [3]. It has many possible uses on the biomedical field[4] [5]and has also been investigated as a potential anti-cancer drug. However, its effects on neuroblastoma (NB) cells is still a mystery. This research aimed to elucidate the effects of Isatin on neuroblastoma cells metastasis and invasion and the underlying mechanism. Neuroblastoma cells viability was tested by CCK8. NB cells invasion and migration ability were tested by transwell and wound healing experiment. The mRNA relative expression of related molecules are detected by Rt-PCR and q-PCR. The protein relative expression of related molecules are detected by Simple western blotting. Our results demonstrated that isatin could inhibit neuroblastoma cell proliferation, invasion, and migration in a dose-dependent manner. Moreover, isatin increases the expression level of H3K4m1, PTEN. All results support the potential anti-metastatic effect of isatin in neuroblastoma cells.

show abstract

Amplified Anodic Electrogenerated Chemiluminescence of Tris(2,2′‐bipyridyl)ruthenium(II) for the Sensitive Detection of Isatin

Abdussalam

Guan

2020

ChemElectroChem

View full text Add to dashboard Cite

Isatin is an endogenous indole derivative that has been reported to possess various pharmacological and therapeutic activities of clinical importance. To date, isatin has been detected mainly by mass spectrometry. Here, we report for the first time the intense anodic electrochemiluminescence (ECL) of tris(2,2′‐bipyridyl)ruthenium(II) with isatin as an efficient co‐reactant. Interestingly, the ECL response of the Ru(bpy)32+/isatin system in borate buffer solution is about 12 times more intense than in phosphate buffer of the same pH; and approximately 114 times greater than the ECL intensity of the luminophore. Under the optimal conditions, the ECL of Ru(bpy)32+ increases linearly with the concentration of the isatin in the range of 1.0 μM to 1.0 mM (R2=0.998) with a limit of detection (LOD) of 0.28 μM (3σ/m). Moreover, the method has been successfully applied to the detection of isatin in human urine. The developed method has shown excellent recoveries in the range of 99.7–101.3 %. The anodic ECL assay reported in this work is superior in terms of linear range and selectivity than the previously reported methods and can be applied for the detection of isatin in real samples.

show abstract

Isatin inhibits SH-SY5Y neuroblastoma cell invasion and metastasis through MAO/HIF-1α/CXCR4 signaling

Cited by 14 publications

References 32 publications

Isatin, an endogenous nonpeptide biofactor: A review of its molecular targets, mechanisms of actions, and their biomedical implications

Isatin, an endogenous nonpeptide biofactor: A review of its molecular targets, mechanisms of actions, and their biomedical implications

Isatin Inhibits SH-SY5Y Neuroblastoma Cell Invasion and Metastasis through LSD1 Activity Inhibition

Amplified Anodic Electrogenerated Chemiluminescence of Tris(2,2′‐bipyridyl)ruthenium(II) for the Sensitive Detection of Isatin

Contact Info

Product

Resources

About