IS902, an insertion element of the chronic-enteritis-causing Mycobacterium avium subsp. silvaticum

Moss, Maurice O.; Malik, Z.; Tizard, Mark; Green, Elizabeth P.; Sanderson, Jeremy; Hermon‐Taylor, John

doi:10.1099/00221287-138-1-139

Cited by 81 publications

(55 citation statements)

References 24 publications

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“…paratuberculosis genome. Previous studies have suggested that the arrangement at some loci is one in which IS900 inserts between the RBS and start codon of the target gene such that the hed ORF, which contains its own start codon within IS900, comes under the control of the upstream host promoter (Tizard et al, 1992 ;Doran et al, 1997). In the present paper, we have used the term ' hed orientation ' to describe the situation in which the predicted direction of genomic transcription at the target locus is in the same direction as hed.…”

Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

“…avium strain 104 genome, but a  search with ORFs either side of locus 6 showed homology (38 % identity) to a polyketide synthase of M. tuberculosis (pks17 : Rv1663) and a transcription regulator (38 % identity) in S. coelicolor (tetR : SCJ11.45c). Loci 4, 6, 8 and 11 corresponded to the IS900 insertions previously described as pMB22, pMBJ3, pMBJ2 and pMBL15, respectively (McFadden et al, 1987 ;Green et al, 1989 ;Moss et al, 1992 ;Hernandez Perez et al, 1994). In addition, although minor sequence differences were observed, locus 3 was equivalent to pMB55.…”

Section: Blast Analysismentioning

confidence: 99%

“…IS900, which is unique for M. avium subsp. paratuberculosis, encodes a 399 aa putative transposase, p43, on one strand (Tizard et al, 1992) and a predicted protein, Hed, of unknown function on the opposite strand (Doran et al, 1994). IS900 inserts in one direction into a consensus target sequence at highly conserved loci within the M. avium subsp.…”

Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

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Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

et al. 2000

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Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was characterized and homologues in the Mycobacterium tuberculosis and M. avium subsp. avium genomes were identified. These included regions encoding a sigma factor (sigJ) at locus 3, a nitrate reductase (nirA) at locus 4, a transcription regulator (tetR) and polyketide synthase at locus 6, and a 6-O-methylguanine methyltransferase at locus 9. In addition, locus numbers were assigned to 9 of 15 RFLP bands previously described. IS900 insertion at 7 of the 14 characterized loci was into the RBS of a gene substituting an RBS encoded by IS900 sited two bases closer to the initiation codon. IS900 insertion at five loci interrupted an ORF at the target site, one of which encoded a homologue of the immunodominant mycobacterial DesA1 protein. Eleven of eighty-one M. avium subsp. paratuberculosis isolates lacked the insertion site at locus 6 together with flanking genomic DNA. This region was also absent from seven reference strains of M. avium subsp. avium, from one M. avium subsp. silvaticum and from six other mycobacterial species. A multiplex PCR of IS900 loci (MPIL) typing method was developed which was able to discriminate 10 different types of M. avium subsp. paratuberculosis from the panel of 81 isolates with consistent differences between those of bovine and ovine origin. Nine MPIL types corresponded with a single PstI/BstEII RFLP type, suggesting that this method may be applicable to typing of M. avium subsp. paratuberculosis directly from a sample without the need for culture. The remaining MPIL type corresponded with seven PstI/BstEII RFLP types. Further resolution of these may come from sequencing the remaining four uncharacterized IS900 loci.

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Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

Section: Blast Analysismentioning

confidence: 99%

Section: Abbreviation : Mpil Multiplex Pcr Of Is900 Locimentioning

confidence: 99%

See 1 more Smart Citation

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

et al. 2000

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“…During the recent genetic analysis of several mycobacterial species, various repetitive DNA elements have been found; many of them have features in common with typical IS elements (Cirillo et al, 1991;Eisenach et al, 1990;Green et al, 1989;Kunze et al, 1991 ;Martin et al, 1990;McAdam et al, 1990;Moss et al, 1992;Patel et al, 1990;Thierry et al, 1990;Woods & Cole, 1990). Transposable elements can be of value as genetic tools.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an IS-like element from Mycobacterium tuberculosis

Mariani

Piccolella

Colizzi

et al. 1993

Journal of General Microbiology

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A DNA sequence, present in members of the Mycobacterium tuberculosis complex, has been identified and characterized. The distribution of this DNA sequence among mycobacterial species was analysed by DNA hybridization and PCR experiments. As the sequence was detected only in bacteria belonging to the M. tuberculosis complex, it may be useful for the rapid discrimination of mycobacteria. Interestingly, the sequence has some characteristics of an insertion element (IS) and codes for a hypothetical protein with significant homologies to proteins encoded by several IS elements of other organisms, namely IS427 and IS869 from Agrobacterium tumefaciens, IS402 from Pseudomonas cepacia, Tn4811 from Streptomyces liuidans and ISRm4 from Rhizobium meliloti. Together, these elements form a previously unrecognized family of transposable elements. This finding suggests the possibility of horizontal gene transfer between pathogenic mycobacteria and other organisms including Gram-negative plant-pathogenic bacteria.

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“…8 The other 2 M. avium subspecies also appear to have 1 or 2 unique sequences (IS901 and IS902) although these do not always seem to be present. 12,14 A general prerequisite for a sensitive and specific serologic test is a specific test antigen. Mycobacterium a. paratuberculosis has a wide range of antigenic determinants, some of which may also be found in Eschericia coli, 7 Nocardia asteroides, corynebacteria, and other mycobacteria.…”

Section: Comparison Of Two Enzyme-linked Immunosorbent Assays For Sermentioning

confidence: 99%

Comparison of Two Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Paratuberculosis (Johne's Disease) in Cattle Using Different Subspecies Strains of Mycobacterium Avium

Nielsen¹,

Houe

Thamsborg

et al. 2001

J VET Diagn Invest

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Serologic diagnosis of bovine paratuberculosis (Johne's disease) with currently available tests may give false-positive results due to cross-reactions with avian and bovine tuberculosis viruses and other infectious agents. Indirect enzyme-linked immunosorbent assays (ELISA) for detection of antibodies against paratuberculosis based on antigens from Mycobacterium avium subsp. avium (A-ELISA) and M. avium subsp. paratuberculosis (P-ELISA) were compared. Despite an expected higher specificity for M. a. paratuberculosis in the P-ELISA, the 2 antigens were equally suitable for demonstration of antibody to M. a. paratuberculosis in cattle. Receiver operating characteristic (ROC) curves was used to demonstrate the possible antigenic relationship. The area under the curve (AUC) was calculated for each of the 2 ROC curves. The AUC for the P-ELISA ROC curve was 0.9197, and the AUC for the A-ELISA ROC curve was 0.9149, demonstrating a negligible difference in efficiency of the 2 tests ( z = 0.182).

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IS902, an insertion element of the chronic-enteritis-causing Mycobacterium avium subsp. silvaticum

Cited by 81 publications

References 24 publications

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.

Characterization of an IS-like element from Mycobacterium tuberculosis

Comparison of Two Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Paratuberculosis (Johne's Disease) in Cattle Using Different Subspecies Strains of Mycobacterium Avium

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