IS231A from Bacillus thuringiensis is functional in Escherichia coli: transposition and insertion specificity

Hallet, Bernard; Rezsöhazy, René; Delcour, Jean

doi:10.1128/jb.173.14.4526-4529.1991

Cited by 26 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most transposons appear to insert randomly into DNA. Others insert preferentially into sites governed by aspects of DNA structure such as bent DNA (Hallet et al, 1991; Pribil and Haniford, 2003), single-nucleotide mismatches (Yanagihara and Mizuuchi, 2002), nucleosomes (Pryciak and Varmus, 1992), or specific chromatin-associated proteins such as Sir4 (Zhu et al, 2003). Yet others insert specifically into predetermined sites; the bacterial Tn7 transposon, in its TnsD-dependent pathway in Escherchia coli , inserts between the phoS and glmS genes, a site conserved in many bacteria (Parks and Peters, 2009).…”

Section: Introductionmentioning

confidence: 99%

Resetting the Site: Redirecting Integration of an Insertion Sequence in a Predictable Way

Guynet¹,

Achard²,

Hoang³

et al. 2009

Molecular Cell

View full text Add to dashboard Cite

Target site choice is a complex and poorly understood aspect of DNA transposition despite its importance in rational transposon-mediated gene delivery. Thoughmost transposons choose target sites essentially randomly or with some slight sequence or structural preferences, insertion sequence IS608 from Helicobacter pylori, which transposes using single-stranded DNA, always inserts just 3′ of a TTAC tetranucleotide. Our results from studies on the IS608 transposition mechanism demonstrated that the transposase recognizes its target site by co-opting an internal segment of transposon DNA and utilizes it for specific recognition of the target sites through base-pairing. This suggested a way to redirect IS608 transposition to novel target sites. As we demonstrate here, we can now direct insertions in a predictable way into a variety of different chosen target sequences, both in vitro and in vivo.

show abstract

Section: Introductionmentioning

confidence: 99%

Resetting the Site: Redirecting Integration of an Insertion Sequence in a Predictable Way

Guynet¹,

Achard²,

Hoang³

et al. 2009

Molecular Cell

View full text Add to dashboard Cite

show abstract

“…Only very few IS 4 elements have been studied in detail. IS 231A has been shown to transpose in vivo by a 'cut-and-paste' mechanism [18], both in its natural host, Bacillus thuringiensis [19], and in Escherichia coli [20]; and it displays a certain degree of insertion specificity [21]. Both IS 10R and IS 50R are part of composite transposons (Tn 10 [22,23] and Tn 5 [24,25], respectively) and are the only members for which in vitro systems have been set up.…”

Section: Introductionmentioning

confidence: 99%

IS4 family goes genomic

2008

View full text Add to dashboard Cite

BackgroundInsertion sequences (ISs) are small, mobile DNA entities able to expand in prokaryotic genomes and trigger important rearrangements. To understand their role in evolution, accurate IS taxonomy is essential. The IS4 family is composed of ~70 elements and, like some other families, displays extremely elevated levels of internal divergence impeding its classification. The increasing availability of complete genome sequences provides a valuable source for the discovery of additional IS4 elements. In this study, this genomic database was used to update the structural and functional definition of the IS4 family.ResultsA total of 227 IS4-related sequences were collected among more than 500 sequenced bacterial and archaeal genomes, representing more than a three fold increase of the initial inventory. A clear division into seven coherent subgroups was discovered as well as three emerging families, which displayed distinct structural and functional properties. The IS4 family was sporadically present in 17 % of analyzed genomes, with most of them displaying single or a small number of IS4 elements. Significant expansions were detected only in some pathogens as well as among certain extremophiles, suggesting the probable involvement of some elements in bacterial and archaeal adaptation and/or evolution. Finally, it should be noted that some IS4 subgroups and two emerging families occurred preferentially in specific phyla or exclusively inside a specific genus.ConclusionThe present taxonomic update of IS4 and emerging families will facilitate the classification of future elements as they arise from ongoing genome sequencing. Their narrow genomic impact and the existence of both IS-poor and IS-rich thriving prokaryotes suggested that these families, and probably ISs in general, are occasionally used as a tool for genome flexibility and evolution, rather than just representing self sustaining DNA entities.

show abstract

“…Changes in topology induced by the nucleoid protein, H-NS, for example, may explain the effects of H-NS mutants on the target choice of IS903 and Tn10 (IS10) (107) (113,114).…”

Section: Target Choicementioning

confidence: 99%

Everyman's Guide to Bacterial Insertion Sequences

et al. 2015

View full text Add to dashboard Cite

The number and diversity of known prokaryotic insertion sequences (IS) have increased enormously since their discovery in the late 1960s. At present the sequences of more than 4000 different IS have been deposited in the specialized ISfinder database. Over time it has become increasingly apparent that they are important actors in the evolution of their host genomes and are involved in sequestering, transmitting, mutating and activating genes, and in the rearrangement of both plasmids and chromosomes. This review presents an overview of our current understanding of these transposable elements (TE), their organization and their transposition mechanism as well as their distribution and genomic impact. In spite of their diversity, they share only a very limited number of transposition mechanisms which we outline here. Prokaryotic IS are but one example of a variety of diverse TE which are being revealed due to the advent of extensive genome sequencing projects. A major conclusion from sequence comparisons of various TE is that frontiers between the different types are becoming less clear. We detail these receding frontiers between different IS-related TE. Several, more specialized chapters in this volume include additional detailed information concerning a number of these.In a second section of the review, we provide a detailed description of the expanding variety of IS, which we have divided into families for convenience. Our perception of these families continues to evolve and families emerge regularly as more IS are identified. This section is designed as an aid and a source of information for consultation by interested specialist readers.

show abstract

IS231A from Bacillus thuringiensis is functional in Escherichia coli: transposition and insertion specificity

Cited by 26 publications

References 17 publications

Resetting the Site: Redirecting Integration of an Insertion Sequence in a Predictable Way

Resetting the Site: Redirecting Integration of an Insertion Sequence in a Predictable Way

IS4 family goes genomic

Everyman's Guide to Bacterial Insertion Sequences

Contact Info

Product

Resources

About