IS1-mediated mobility of the aerobactin system of pColV-K30 in Escherichia coli

Herrero, Marta; Neilands, J. B.

doi:10.1007/bf00339620

Cited by 21 publications

(12 citation statements)

References 30 publications

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“…4A). In addition, an aerobactin iron-acquisition siderophore system (lutA and lucABC) (12) and an iron transporter operon (sitABCD) (34) were flanked by two IS1-2 elements, and a possible transposon was also identified in plasmid pCVM29188_146 of S. enterica serovar Kentucky from poultry (15) and plasmids in avian pathogenic Escherichia coli (11,25).…”

Section: Resultsmentioning

confidence: 99%

Whole-Genome Analysis of Salmonella enterica Serovar Typhimurium T000240 Reveals the Acquisition of a Genomic Island Involved in Multidrug Resistance via IS 1 Derivatives on the Chromosome

Izumiya

Sekizuka²,

Nakaya

et al. 2011

Antimicrob Agents Chemother

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Salmonella enterica serovar Typhimurium is frequently associated with life-threatening systemic infections, and the recent global emergence of multidrug resistance in S. enterica isolates from agricultural and clinical settings has raised concerns. In this study, we determined the whole-genome sequence of fluoroquinoloneresistant S. enterica serovar Typhimurium T000240 strain (DT12) isolated from human gastroenteritis in 2000. Comparative genome analysis revealed that T000240 displays high sequence similarity to strain LT2, which was originally isolated in 1940, indicating that progeny of LT2 might be reemerging. T000240 possesses a unique 82-kb genomic island, designated as GI-DT12, which is composed of multidrug resistance determinants, including a Tn2670-like composite transposon (class 1 integron [intI1, bla oxa-30 , aadA1, qacE⌬1, and sul1], mercury resistance proteins, and chloramphenicol acetyltransferase), a Tn10-like tetracycline resistance protein (tetA), the aerobactin iron-acquisition siderophore system (lutA and lucABC), and an iron transporter (sitABCD). Since GI-DT12 is flanked by IS1 derivatives, IS1-mediated recombination likely played a role in the acquisition of this genomic island through horizontal gene transfer. The aminoglycoside-(3)-N-acetyltransferase (aac(3)) gene and a class 1 integron harboring the dfrA1 gene cassette responsible for gentamicin and trimethoprim resistance, respectively, were identified on plasmid pSTMDT12_L and appeared to have been acquired through homologous recombination with IS26. This study represents the first characterization of the unique genomic island GI-DT12 that appears to be associated with possible IS1-mediated recombination in S. enterica serovar Typhimurium. It is expected that future whole-genome studies will aid in the characterization of the horizontal gene transfer events for the emerging S. enterica serovar Typhimurium strains.

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Section: Resultsmentioning

confidence: 99%

Whole-Genome Analysis of Salmonella enterica Serovar Typhimurium T000240 Reveals the Acquisition of a Genomic Island Involved in Multidrug Resistance via IS 1 Derivatives on the Chromosome

Izumiya

Sekizuka²,

Nakaya

et al. 2011

Antimicrob Agents Chemother

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“…Despite its superficial similarities to a transposon, the aerobactin region has not been shown to undergo transposition (85,596). It seems unlikely that the aerobactin region has functioned as a transposon in recent evolutionary history (458) because the insertion sequences are not in the usual orientation, the region is too large, and the conserved region extends beyond the insertion sequences (596).…”

Section: Geneticsmentioning

confidence: 99%

Virulence factors in Escherichia coli urinary tract infection

1991

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Uropathogenic strains of Escherichia coli are characterized by the expression of distinctive bacterial properties, products, or structures referred to as virulence factors because they help the organism overcome host defenses and colonize or invade the urinary tract. Virulence factors of recognized importance in the pathogenesis of urinary tract infection (UTI) include adhesins (P fimbriae, certain other mannose-resistant adhesins, and type 1 fimbriae), the aerobactin system, hemolysin, K capsule, and resistance to serum killing. This review summarizes the virtual explosion of information regarding the epidemiology, biochemistry, mechanisms of action, and genetic basis of these urovirulence factors that has occurred in the past decade and identifies areas in need of further study. Virulence factor expression is more common among certain genetically related groups of E. coli which constitute virulent clones within the larger E. coli population. In general, the more virulence factors a strain expresses, the more severe an infection it is able to cause. Certain virulence factors specifically favor the development of pyelonephritis, others favor cystitis, and others favor asymptomatic bacteriuria. The currently defined virulence factors clearly contribute to the virulence of wild-type strains but are usually insufficient in themselves to transform an avirulent organism into a pathogen, demonstrating that other as-yet-undefined virulence properties await discovery. Virulence factor testing is a useful epidemiological and research tool but as yet has no defined clinical role. Immunological and biochemical anti-virulence factor interventions are effective in animal models of UTI and hold promise for the prevention of UTI in humans.

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“…Aerobactin genes have been found in the chromosomes of different bacterial species and in several different plasmids, a situation which indicates easy mobilization. The aerobactin operon forms part of a transposable element (102), and it has been proposed that it is part of a pathogenicity island in Shigella flexneri (403). Other virulence determinants found in transposable elements are the E. coli enterotoxin STII gene (170), the Shiga toxin operon, which has been found in a putative composite transposon in Shigella dysenteriae 1 (257), and the toxic shock toxin, carried by a family of mobile pathogenicity islands in Staphylococcus aureus (218).…”

Section: Evolution and Dissemination Of Genes Involved In Virulence Amentioning

confidence: 99%

Interactions among Strategies Associated with Bacterial Infection: Pathogenicity, Epidemicity, and Antibiotic Resistance

2002

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SUMMARY Infections have been the major cause of disease throughout the history of human populations. With the introduction of antibiotics, it was thought that this problem should disappear. However, bacteria have been able to evolve to become antibiotic resistant. Nowadays, a proficient pathogen must be virulent, epidemic, and resistant to antibiotics. Analysis of the interplay among these features of bacterial populations is needed to predict the future of infectious diseases. In this regard, we have reviewed the genetic linkage of antibiotic resistance and bacterial virulence in the same genetic determinants as well as the cross talk between antibiotic resistance and virulence regulatory circuits with the aim of understanding the effect of acquisition of resistance on bacterial virulence. We also discuss the possibility that antibiotic resistance and bacterial virulence might prevail as linked phenotypes in the future. The novel situation brought about by the worldwide use of antibiotics is undoubtedly changing bacterial populations. These changes might alter the properties of not only bacterial pathogens, but also the normal host microbiota. The evolutionary consequences of the release of antibiotics into the environment are largely unknown, but most probably restoration of the microbiota from the preantibiotic era is beyond our current abilities.

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IS1-mediated mobility of the aerobactin system of pColV-K30 in Escherichia coli

Cited by 21 publications

References 30 publications

Whole-Genome Analysis of Salmonella enterica Serovar Typhimurium T000240 Reveals the Acquisition of a Genomic Island Involved in Multidrug Resistance via IS 1 Derivatives on the Chromosome

Whole-Genome Analysis of Salmonella enterica Serovar Typhimurium T000240 Reveals the Acquisition of a Genomic Island Involved in Multidrug Resistance via IS 1 Derivatives on the Chromosome

Virulence factors in Escherichia coli urinary tract infection

Interactions among Strategies Associated with Bacterial Infection: Pathogenicity, Epidemicity, and Antibiotic Resistance

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