Is Your Açaí Really from Amazon? Using DNA Barcoding to Authenticate Commercial Products

Lugon, Magda Delorence; Santos, Pedro Henrique Dias dos; Oliveira, Pablo Viana; Almeida, Francine Alves Nogueira de; Luber, Jaquelini; Forzza, Rafaela Campostrini; Jardim, Mário Augusto Gonçalves; Paneto, Greiciane Gaburro

doi:10.1007/s12161-021-01998-2

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…Its fruit is known as açai ( Fig. 1 A), and its pulp can be obtained mainly from the fruits of two palm species, Euterpe oleracea and Euterpe precatoria [ 7 ]. Because they are morphologically similar species, the main differentiating factor is how the palm trees grow; E. precatoria has only a single thin stalk, and E. oleracea has multiple stalks [ 8 ].…”

Section: Fruits Of Amazonian Palm Treesmentioning

confidence: 99%

Amazonian palm tree fruits: From nutritional value to diversity of new food products

Amorim,

Godoy

et al. 2024

Heliyon

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Section: Fruits Of Amazonian Palm Treesmentioning

confidence: 99%

Amazonian palm tree fruits: From nutritional value to diversity of new food products

Amorim,

Godoy

et al. 2024

Heliyon

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“…Euterpe edulis Martius is a dominant palm species in the understory of the Atlantic Forest and the Cerrado of South America. Repeated cycles of exploitation aimed at extracting the palm heart, whose quality and avor are superior to those of other Euterpe species, have led to a decline in this species' population (Borges et al 2011;Rother et al 2016; Barroso et al 2019;Lugon et al 2021). Given that E. edulis is a key species for the structure and dynamics of the Atlantic Forest, conservation efforts should focus on a more sustainable use of this resource (Rother et al 2016; Souza and Prevedello 2020).…”

Section: Introductionmentioning

confidence: 99%

Maturation and quality of seeds of an endangered tropical palm species (Euterpe edulis Martius) assessed by imaging and X-ray densitometry

Mello

Zanardo

Taliuli

et al. 2023

Preprint

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Euterpe edulis Martius is an endangered palm species that grows in the Atlantic Forest and the Cerrado of South America. Economic exploitation of its antioxidant-rich fruits could ensure the sustainable management of this species. However, this relies on the rapid selection of high-quality seeds from which to derive seedlings. The objective of this study was to investigate the maturation of E. edulis seeds using image analysis and X-ray densitometry. Fruits were harvested from ten matrices at different stages of maturation, from 94 days after anthesis (DAA) to 290 DAA. Seed dry mass, water content, germination, vigor, and density were quantified at each stage. At the same time, seeds were analyzed by GroundEye® imaging, radiography, scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). The highest dry mass was detected 255 DAA (0.83 g), in vitro germination began 115 DAA and achieved 100% from 150 DAA, and maximum vigor was observed 164 DAA, whereby 100% of seedlings appeared normal. X-ray imaging revealed dehydrated seeds and small mechanical damage, such as cracking of the pericarp. X-ray densitometry revealed that seed density increased considerably 185 DAA. SEM/EDS detected changes between maturation stages, such as the accumulation of K and Si, in the mesocarp and endocarp. Overall, E. edulis seeds presented maximum in vitro germination, vigor, percentage of normal seedlings, and physicochemical qualities 164 DAA (green epicarp), which corresponds to 126 days earlier compared with the fruits harvested 290 DAA (black epicarp) for ex vitro germination.

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Evaluation of the Plastid and Nuclear DNA barcodes in genus Ocimum towards Quality Assurance in Herbal Industry

Kumar,

Rodrigues,

Saxena

et al. 2023

Preprint

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Background: Species of the genus Ocimumare of immense value and are in high demand in trade which leads to unscrupulous adulteration of both crude drugs as well as formulations. Traditional method-based authentication is difficult in case of incomplete or damaged samples and in dried herbal bulk material. High degree of morphological similarity, issues of polyploidy and the possibility of inter- and intraspecific hybridization have plagued the Ocimum market. Hence, there is an immediate need for developing DNA barcodes for fast and accurate identification of the species. Results: In this study, three plastid regions (matK, rbcL and trnH-psbA) and two nuclear regions (ITS and ITS2) are evaluated for their performance as DNA barcodes to check the delineation of Ocimum species. Nearest genetic distance, Wilcoxon test, Best Match (BM) and Neighbor-Joining (NJ) tree methods were taken into account to assess the efficiency of proposed DNA barcodes. Our study suggests that discrimination rate amongst single barcodes is the highest for trnH-psbA with the NJ analysis. By combining two or more barcodes, no significant changes were observed. Conclusion: A reliable and commercially viable DNA barcoding system has been developed for accurate species identification of various Ocimum species. The varied approaches used in the investigation had different species identification potential. The identification success rates of plastid DNA and nuclear DNA barcodes were comparable using pairwise genetic distance (PWG-distance), BM and NJ methods. Despite having high inter-specific and the lowest intra-specific genetic distance, trnH-psbAfailed to discriminate Ocimum species having hybrid origin, indicating the need to develop more suitable barcode loci in future.

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Is Your Açaí Really from Amazon? Using DNA Barcoding to Authenticate Commercial Products

Cited by 3 publications

References 46 publications

Amazonian palm tree fruits: From nutritional value to diversity of new food products

Amazonian palm tree fruits: From nutritional value to diversity of new food products

Maturation and quality of seeds of an endangered tropical palm species (Euterpe edulis Martius) assessed by imaging and X-ray densitometry

Evaluation of the Plastid and Nuclear DNA barcodes in genus Ocimum towards Quality Assurance in Herbal Industry

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