Is urinary 5-hydroxyindoleacetic acid helpful for early diagnosis of acute appendicitis?

Jangjoo, Ali; Varasteh, Abdolreza; Bahar, Mostafa Mehrabi; Meibodi, Naser Tayyebi; Esmaili, Habibollah; Nazeri, Narges; Aliakbarian, Mohsen; Azizi, Shahriar H.

doi:10.1016/j.ajem.2011.01.027

Cited by 22 publications

(31 citation statements)

References 14 publications

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“…Its accurate preoperative diagnosis still remains elusive. The overall negative laparotomy rate remains at about 15-30% [3]. In women of a childbearing age, this rate is nearly doubled because of the prevalence of gynecologic diseases, the figure being as high as 30-50% [4].…”

Section: Introductionmentioning

confidence: 91%

Serum Biomarkers As a Diagnostic Aid in Acute Appendicitis

Jairaj

Narendranath

Ali

et al. 2016

Int J of Biomed & Adv Res

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Background: Acute appendicitis is one of the most common acute intra-abdominal infections seen in surgical departments, which can be treated easily if an accurate diagnosis is made on time. Otherwise, delay in diagnosis and treatment can lead to perforation of the appendix &diffuse peritonitis. Materials and Methods: A prospective study was conducted on 112 patients who were operated for acute appendicitis in department of General surgery, Bowring & Lady Curzon Hospitals, BMCRI, to determine the role and predictive value of the total leucocyte count (TLC), C-reactive protein (CRP) and percentage of neutrophil count in the diagnosis of acute appendicitis. Preoperative TLC, CRP and percentage of neutrophil count were determined and were compared with the results of the histopathology of the removed appendix. Results: Of all the patients studied, 94 had histopathologically positive appendicitis. The TLC was found to be significantly high in 82 patients who proved to have acute appendicitis, whereas CRP was high in 88 patients and neutrophil percentage was raised in 87 patients; 6 patients had a normal CRP level. Thus, TLC had a sensitivity, specificity &positive predictive value of 87.23%, 44.44% and 89.13% respectively. CRP had a sensitivity, specificity & positive predictive value of 93.61%, 77.77% and 95.65% respectively. Percentage of neutrophil count had a sensitivity, specificity & positive predictive value of 92.55%, 38.88%, 88.77% respectively. When used in combination, there was a marked improvement in the sensitivity, specificity and the positive predictive value to98.76%, 86.66% and 97.56% respectively. Conclusion: The inflammatory markers, i.e., TLC, CRP and neutrophil count can be helpful in the diagnosis when measured together as this increases their sensitivity, specificity & positive predictive value significantly.

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Section: Introductionmentioning

confidence: 91%

Serum Biomarkers As a Diagnostic Aid in Acute Appendicitis

Jairaj

Narendranath

Ali

et al. 2016

Int J of Biomed & Adv Res

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“…Previous studies have indicated that appendix is enriched with enterochromaffin cells (11). These cells are densely concentrated with serotonin.…”

Section: Introductionmentioning

confidence: 99%

Spot urinary 5-hydroxyindoleacetic acid is not an ideal diagnostic test for acute appendicitis

Rao

Wilson

Kennedy

et al. 2016

The American Journal of Emergency Medicine

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“…The 24-hour urine 5-HIAA test performed with the commercial "kit" is an alternative to laboratories lack of equipment for conducting the HPLC technique, which is most employed (1,4,5) . The colorimetric assay proposed by Goldenberg (3) is faster than the HPLC method, especially for routine analysis for a high number of samples.…”

Section: Introductionmentioning

confidence: 99%

“…Quantifying urinary 5-hydroxyindoleacetic acid (5-HIAA), the main metabolite of serotonin, is a useful marker for screening and monitoring carcinoid tumors, and also helps in the differentiation of acute appendicitis from other abdominal pain etiologies (1,4,5) .…”

Section: Introductionmentioning

confidence: 99%

“…The 24-hour urine 5-HIAA test can be determined by highperformance liquid chromatography (HPLC), the colorimetric assay proposed by Goldenberg (3) in 1973 that uses nitrosonaphthol and 2-mercaptoethanol, enzyme immunoassay, and gas chromatography-mass spectrometry (1,3,5) . The 24-hour urine 5-HIAA test performed with the commercial "kit" is an alternative to laboratories lack of equipment for conducting the HPLC technique, which is most employed (1,4,5) .…”

Section: Introductionmentioning

confidence: 99%

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Quantification of urinary 5-hydroxyindoleacetic acid by in-house nitrosonaphthol reaction compared with nitrosonaphthol micro column chromatography and enzyme-linked immunosorbent assay

Silva¹,

Mattar²,

Colaúto³

et al. 2014

Jornal Brasileiro De Patologia E Medicina Laboratorial

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The aim of this study was to compare the colorimetric "kit" and enzyme-linked immunosorbent assay (ELISA) methods to quantify urinary 5-hydroxyindoleacetic acid through the Goldenberg's technique, exploring the potential of replacing it. 24-hour urine samples were tested by Goldenberg's assay and compared with kits. The agreement was almost perfect for the comparison of Goldenberg's assay with both colorimetric kit, and with ELISA kit, considering ≤ 7.5 mg/24h normal cutoff value. Therefore, both "kits" would be good alternatives to Goldenberg's technique due to practicality and agreement between values.

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Is urinary 5-hydroxyindoleacetic acid helpful for early diagnosis of acute appendicitis?

Cited by 22 publications

References 14 publications

Serum Biomarkers As a Diagnostic Aid in Acute Appendicitis

Serum Biomarkers As a Diagnostic Aid in Acute Appendicitis

Spot urinary 5-hydroxyindoleacetic acid is not an ideal diagnostic test for acute appendicitis

Quantification of urinary 5-hydroxyindoleacetic acid by in-house nitrosonaphthol reaction compared with nitrosonaphthol micro column chromatography and enzyme-linked immunosorbent assay

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