Is TISCH a suitable tracer for in vivo study of modifications of the dopamine D-1 receptor?

Pellevoisin, Christian; Chalon, Sylvie; Kung, Hank F.; Besnard, Jean‐Claude; Guilloteau, Denis

doi:10.1016/0969-8051(95)02070-5

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Using an in vitro autoradiographic method with [3HlSCH 23390 to evaluate striatal dopamine D1 receptors, we found a 70% decrease in the binding of the ligand on the lesioned striatum compared to the intact side. A similar decrease was already observed with ['2511TISCH (Pellevoisin et al, 1996), a selective iodinated ligand for D1 receptors (Billings et al, 1992). TISCH could therefore be used in SPECT explorations as a direct marker of neuronal loss; however, neuronal loss might be detected indirectly with a ligand of non- Paxinos and Watson, [19821).…”

Section: Discussionsupporting

confidence: 65%

Iodinated PK 11195 as an ex vivo marker of neuronal injury in the lesioned rat brain

Chalon

Pellevoisin

Bodard

et al. 1996

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In order to study the potentials of indirect and direct detection of neuronal damages in humans by single photon emission computerized tomography (SPECT), we compared ex vivo cerebral biodistribution of [125I]PK 11195 with that of [125I]TISCH in a rat model of unilateral striatal excitotoxic lesion. Experiments on in vitro binding with [3H]PK 11195 as a ligand for peripheral type benzodiazepine binding sites (PTBBS) and [3H]SCH 23390 as a ligand for dopamine D1 receptors were also performed to validate our experimental model. We observed a very high increase in the PTBBS and a dramatic decrease in the D1 receptors on the lesioned striatum compared to the intact side. Moreover, we showed that [125I]PK 11195 bound specifically to PTBBS in lesioned cerebral areas ex vivo 5 days after striatal infusion of quinolinic acid (600 nmoles). Increases of 192%, 168%, and 30% were obtained in the striatum, the cortex, and the hippocampus, respectively, on the lesioned side. These results showed that iodinated PK 11195 bound specifically to PTBBS ex vivo and that this binding was dramatically increased at the sites of brain lesion. This ligand could therefore be suitable to detect brain injuries in humans by SPECT, complementing the information given by ligands which image loss in a specific neuron population.

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Section: Discussionsupporting

confidence: 65%

Iodinated PK 11195 as an ex vivo marker of neuronal injury in the lesioned rat brain

Chalon

Pellevoisin

Bodard

et al. 1996

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The Effect of Docarpamine, a Dopamine Pro-Drug, on Blood Pressure and Catecholamine Levels in Spontaneously Hypertensive Rats

Sanada

Asico

Shigetomi

et al. 2000

Clinical and Experimental Hypertension

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We studied the effects of bolus intravenous injection of the dopamine prodrug, docarpamine (200 microg/kg), on mean arterial pressure (MAP) and heart rate (HR) in Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHRs). In WKY rats (n=18), MAP and HR increased 5 min after docarpamine and then returned to baseline levels within 15 min. In contrast, in SHRs (n=15), MAP and HR gradually decreased, reaching a nadir 20 min after injection. Five min after docarpamine, plasma dopamine and 3,4-dihydroxy phenyl acetic (DOPAC) levels increased in both WKY rats (n=5) and SHRs (n=5). The docarpamine-induced changes in MAP and HR in both rat strains (n=5/strain) were blocked by the D1-like antagonist, SCH23390. alpha-Adrenergic (n=4) and vasopressin V1 (n=3) receptor blockade also abrogated the effects of docarpamine in WKY rats. We conclude that docarpamine differentially affects MAP and HR in WKY and SHRs. In SHRs, the depressor and bradycardiac effects of docarpamine are mediated by D1-like receptors. In WKY rats, the pressor and tachycardiac responses are caused by an interaction among D1-like, alpha-adrenergic, and V1 receptors.

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Is TISCH a suitable tracer for in vivo study of modifications of the dopamine D-1 receptor?

Cited by 2 publications

References 14 publications

Iodinated PK 11195 as an ex vivo marker of neuronal injury in the lesioned rat brain

Iodinated PK 11195 as an ex vivo marker of neuronal injury in the lesioned rat brain

The Effect of Docarpamine, a Dopamine Pro-Drug, on Blood Pressure and Catecholamine Levels in Spontaneously Hypertensive Rats

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