Is there a uniform basal endometrial gene expression profile during the implantation window in women who became pregnant in a subsequent ICSI cycle?

Allegra, Adolfo; Marino, Angelo; Coffaro, Francesco; Lama, A.; Rizza, Giorgia; Scaglione, Piero; Sammartano, Francesca; Santoro, Alessandra; Volpes, Aldo

doi:10.1093/humrep/dep222

Cited by 27 publications

(26 citation statements)

References 39 publications

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“…Although functional analysis and studies in knockout mice have demonstrated that all not these genes have a role in the embryonic implantation process (48), they are unquestioned markers of the correct functioning of the endometrium. Indeed, LIF expression has been shown to be statistically uniform among patients who subsequently became pregnant in intracytoplasmic sperm injection cycles (49). We have also detected the dysregulation of other genes, such as CAPN6 and HABP2 in obese women with PCOS, which have recently been shown to be dysregulated during the WOI in women with unexplained infertility (50).…”

Section: Discussionmentioning

confidence: 57%

Endometrial gene expression in the window of implantation is altered in obese women especially in association with polycystic ovary syndrome

Bellver

Martínez-Conejero

Labarta

et al. 2011

Fertility and Sterility

159

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Section: Discussionmentioning

confidence: 57%

Endometrial gene expression in the window of implantation is altered in obese women especially in association with polycystic ovary syndrome

Bellver

Martínez-Conejero

Labarta

et al. 2011

Fertility and Sterility

159

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“…This characteristic profile suggested that a unique transcriptional process occurs to achieve a receptive phenotype (Borthwick et al 2003;Riesewijk et al 2003;Horcajadas et al 2008;Haouzi et al 2009a,b; The implantation of the blastocyst in the endometrium activates the production of cytokines that modulate receptivity by regulating the expression of adhesion molecules in mammals (Simon et al 1997). Leukemia inhibitory factor (LIF) is a cytokine that has been the focus of many studies (Aghajanova et al 2008;Allegra et al 2009;Rashid et al 2011) because of its clear functional effect in the mouse model. Osteopontin (SPP1) has been the gene with the greatest consensus among most endometrial transcriptomic studies.…”

Section: Transcriptomics Of Endometrial Receptivitymentioning

confidence: 99%

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Gómez

Ruiz-Alonso

Miravet

et al. 2015

Cold Spring Harb Perspect Med

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Human endometrium has been extensively investigated in the search for markers capable of predicting its receptive status. The completion of the Human Genome Project has triggered a rapid development of new fields in molecular biology, the "transcriptomics" being a major turning point in the knowledge acquisition of endometrial receptivity. Based on this, a customized Endometrial Receptivity Array (ERA) has been developed, which is capable of identifying the genomic signature of receptivity. This diagnostic tool showed that the window of implantation (WOI) is displaced in one out of four patients with implantation failure, allowing the identification of their personalized WOI. This strategy allows performing a personalized embryo transfer ( pET) on the day in which the endometrium is receptive. The combination of a systems biology approach and next-generation sequencing will overcome the limitations of microarrays, and will, in the future, allow elucidation of the mechanisms involved in embryo implantation.T he endometrium, which lines the inside of the uterine cavity, undergoes cyclic changes that are regulated by ovarian steroids. It can be subdivided into the basal layer that is responsible for its regenerative capacity, and the functional layer that undergoes proliferation, secretion, and tissue degeneration every month from menarche to menopause. Its aim is to prepare the optimal moment for embryonic implantation known as the window of implantation (WOI).The endometrial cycle comprises the menstrual, proliferative, and secretory phases. The proliferative phase, which corresponds to the ovarian follicular phase and increased production of estrogens, lasts until ovulation occurs. During this stage, the increasing estrogen levels cause the proliferation of the stromal cells as well as the elongation of the spiral arteries. After ovulation, with the appearance of progesterone secreted by the granulosa-luteal cells, the secretory phase begins. If implantation does not occur, the secretory phase ends, and the corpus luteum degenerates. Menstruation occurs owing to the drop of estrogen and progesterone, which resets the endometrium until pregnancy

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“…STC is expressed in pre-pregnancy endometrium and early pregnancy decidua in rats and pigs, is regulated by estrogen and progesterone, and has been suggested as an implantation marker in pig endometrium (1, 2). In humans, consistent mid-secretory endometrial (MSE) STC1 gene expression was demonstrated in patients who conceived with the help of assisted reproductive technologies (ART) (3). STC1 was significantly upregulated in microarray analysis of MSE of the normal menstrual cycle compared to early secretory endometrium (ESE) (4), and was down-regulated in microarray analysis of MSE from women with unexplained infertility versus fertile control subjects (5), suggesting a possible role in human endometrial receptivity and implantation.…”

Section: Introductionmentioning

confidence: 99%

Stanniocalcin-1 expression in normal human endometrium and dysregulation in endometriosis

Aghajanova

Altmäe

Kasvandik

et al. 2016

Fertility and Sterility

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Objective To determine expression of stanniocalcin-1 (STC1) in human endometrium with and without endometriosis and its regulation by steroid hormones. Design Laboratory study. Setting University. Patients 19 women with endometriosis and 33 control women. Intervention(s) Endometrial biopsy and fluid sampling Main Outcome Measure(s) Analysis of early secretory (ESE) and mid-secretory (MSE) endometrial secretomes from fertile women with nano-LC/MS/MS; real-time Q-PCR and immunohistochemistry for STC1 and its receptor CASR mRNA and proteins in endometrium with and without endometriosis; evaluation of STC1 and CASR mRNA expression in endometrial stromal fibroblasts (eSF) from women with and without endometriosis decidualized with E2P4 or 8-bromo-cAMP. Results STC1 protein was strongly upregulated (p=0.0004) in MSE versus ESE in endometrial fluid of fertile women. STC1 mRNA significantly increased in MSE from women with, but not without endometriosis, compared to proliferative endometrium (PE) or ESE, with no significant difference throughout the menstrual cycle between groups. STC1 mRNA in eSF from control women increased >230-fold upon decidualization with cAMP versus 45-fold from women with endometriosis, which was not seen upon decidualization with E2/P4. CASR mRNA did not exhibit significant differences in any condition, and was not expressed in isolated eSF. STC1 protein immunoexpression in eSF was significantly lower in women with endometriosis compared to controls. Conclusions STC1 protein is significantly upregulated in MSE endometrial fluid, and is dysregulated in eutopic endometrial tissue from women with endometriosis. It is likely regulated by cAMP and may be involved in the pathogenesis of decidualization defects.

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Is there a uniform basal endometrial gene expression profile during the implantation window in women who became pregnant in a subsequent ICSI cycle?

Cited by 27 publications

References 39 publications

Endometrial gene expression in the window of implantation is altered in obese women especially in association with polycystic ovary syndrome

Endometrial gene expression in the window of implantation is altered in obese women especially in association with polycystic ovary syndrome

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Stanniocalcin-1 expression in normal human endometrium and dysregulation in endometriosis

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