Is there a synergistic effect between mixed bacterial infection in biofilm formation on biliary stents?

Leung, Joseph; Liu, Yan Lei; Desta, Taddese; Libby, Eric; Inciardi, John F.; Lam, Kei Fong

doi:10.1016/s0016-5107(98)70186-5

Cited by 60 publications

(39 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When a biofilm is composed of heterogeneous species (which is more likely in nature than single species), the metabolic by-products of one organism might serve to support the growth of another, while the adhesion of one species might provide ligands allowing the attachment of others (17,60,66,94). Conversely, the competition for nutrients and accumulation of toxic by-products generated by primary colonizers can limit the species diversity within a biofilm (66).…”

Section: Biofilm Diversitymentioning

confidence: 99%

“…Interestingly, the presence of one species of microorganism on a surface can promote the adhesion of another (60,70). All bacteria produce multiple adhesins, and some are regulated at the transcriptional level, permitting organisms to switch from sessile to planktonic forms under different environmental influences (2,96).…”

Section: Locking: Secondary Bacterial Adhesionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Adhesion: Seen Any Good Biofilms Lately?

2002

View full text Add to dashboard Cite

The process of surface adhesion and biofilm development is a survival strategy employed by virtually all bacteria and refined over millions of years. This process is designed to anchor microorganisms in a nutritionally advantageous environment and to permit their escape to greener pastures when essential growth factors have been exhausted. Bacterial attachment to a surface can be divided into several distinct phases, including primary and reversible adhesion, secondary and irreversible adhesion, and biofilm formation. Each of these phases is ultimately controlled by the expression of one or more gene products. Ultrastructurally, the mature bacterial biofilm resembles an underwater coral reef containing pyramidal or mushroom-shaped microcolonies of organisms embedded within an extracellular glycocalyx, with channels and cavities to allow the exchange of nutrients and waste. The biofilm protects its inhabitants from predators, dehydration, biocides, and other environmental extremes while regulating population growth and diversity through primitive cell signals. From a physiological standpoint, surface-bound bacteria behave quite differently from their planktonic counterparts. Recognizing that bacteria naturally occur as surface-bound and often polymicrobic communities, the practice of performing antimicrobial susceptibility tests using pure cultures and in a planktonic growth mode should be questioned. That this model does not reflect conditions found in nature might help explain the difficulties encountered in the management and treatment of biomedical implant infections

show abstract

Section: Biofilm Diversitymentioning

confidence: 99%

Section: Locking: Secondary Bacterial Adhesionmentioning

confidence: 99%

Bacterial Adhesion: Seen Any Good Biofilms Lately?

2002

View full text Add to dashboard Cite

show abstract

“…43 Numerous studies have demonstrated the multispecies nature of microbial biofilm which progressively increases in thickness and contributes significantly to occlusion of the stent lumen. 28,29,40,[43][44][45] To address the issue of how a biofilm could reach such a thickness to significantly narrow the lumen of the stent, one must remember that a biofilm, by definition, is a microbial community embedded within an exopolysaccharide matrix and which also engulfs a number of "foreign bodies" of different sizes. Further, the three-dimensional structure of biofilm recognizes the presence of voids and channels needed for the diffusion of nutrients and signal molecules required for intercellular communication.…”

Section: Causative Factors and Dynamics Of The Clogging Process In Plmentioning

confidence: 99%

Plastic Biliary Stent Occlusion: Factors Involved and Possible Preventive Approaches

Donelli

Guaglianone

Rosa

et al. 2007

Clinical Medicine & Research

137

112

View full text Add to dashboard Cite

“…In our study, all tested strains produced maximum biofilm after 60 hours. This Adhesions of enterotoxigenic E. coli and CfaE-R181A mutant may be due to greater production of glycocalyx in these strains in biofilm mode (14). Also significantly higher biofilm production by E24377a compared to MC4100/pJGX15W and DH5aF'lacI q /pEU588 may be related to presence of two antigenic types (i.e., CS1 and CS3), hence contributing towards greater adherence and or/glycocalyx production.…”

Section: Discussionmentioning

confidence: 93%

“…Also significantly higher biofilm production by E24377a compared to MC4100/pJGX15W and DH5aF'lacI q /pEU588 may be related to presence of two antigenic types (i.e., CS1 and CS3), hence contributing towards greater adherence and or/glycocalyx production. Also significantly high biofilm produced by piliated and recombinant piliated strains may be due to the fact that piliated E. coli organisms are more hydrophobic compared to their nonpiliated counterparts (14). The fact that biofilm formation and adherence was observed in mutant/tip mutant indicates that other adhesins may act simultaneously or at distinct steps of the adherence process (18).…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation and binding specificities of CFA/I, CFA/II and CS2 adhesions of Enterotoxigenic Escherichia coli and CfaE-R181A mutant

Liaqat

Sakellaris²

2012

Braz. J. Microbiol.

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim formation, colonization and host cell adhesions. As a first step, we have determined the biofilm capability of ETEC expressing various types of pili (CFA/I, CfaE-R181A mutant/ CfaE tip mutant, CFA/II and CS2). Further, enzyme-linked immunosorbent assay (ELISA) assay were developed to compare the binding specificity of CFA/I, CFA/II (CS1 -CS3) and CS2 of ETEC, using extracted pili and piliated bacteria. CFA/II strain (E24377a) as well as extracted pili exhibited significantly higher binding both in biofilm and ELISA assays compared to non piliated wild type E24377a, CFA/I and CS2 strains. This indicates that co-expression of two or more CS2 in same strain is more efficient in increasing adherence. Significant decrease in binding specificity of DH5αF'lacI q /∆cotD (CS2) strain and MC4100/pEU2124 (CfaE-R181A) mutant strain indicated the important contribution of tip proteins in adherence assays. However, CS2 tip mutant strain (DH5αF'lacI q /pEU5881) showed that this specific residue may not be important as adhesions in these strains. In summary, our data suggest that pili, their minor subunits are important for biofilm formation and adherence mechanisms. Overall, the functional reactivity of strains co expressing various antigens, particularly minor subunit antigen observed in this study suggest that fewer antibodies may be required to elicit immunity to ETEC expressing a wider array of related pili.

show abstract

Is there a synergistic effect between mixed bacterial infection in biofilm formation on biliary stents?

Cited by 60 publications

References 18 publications

Bacterial Adhesion: Seen Any Good Biofilms Lately?

Bacterial Adhesion: Seen Any Good Biofilms Lately?

Plastic Biliary Stent Occlusion: Factors Involved and Possible Preventive Approaches

Biofilm formation and binding specificities of CFA/I, CFA/II and CS2 adhesions of Enterotoxigenic Escherichia coli and CfaE-R181A mutant

Contact Info

Product

Resources

About