Is there a selectivity of neuronal degeneration induced by intrastriatal injection of kainic acid?

Krammer, Eva B.; Lischka, Martin F.; Karobath, Manfred; ̈nbeck, Georg Scho

doi:10.1016/0006-8993(79)90476-1

Cited by 40 publications

(3 citation statements)

References 20 publications

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“…2bЈЈ,cЈЈ; VGluT2, data not shown). This indicated that most afferents to the neostriatum were preserved in the damaged region, although some changes would have occurred in the afferent terminals (Wuerthele et al, 1978;Krammer et al, 1979). Thus, the present findings suggested that type 1 mGluR4-positive excitatory terminals were of cortical or thalamic origin.…”

Section: Kainic Acid Injection Into the Neostriatummentioning

confidence: 52%

Metabotropic glutamate receptor 4‐immunopositive terminals of medium‐sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum

Kuramoto

Fujiyama

Unzai

et al. 2006

J of Comparative Neurology

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Metabotropic glutamate receptor 4 (mGluR4) is localized mainly to presynaptic membranes in the brain. Rat neostriatum has been reported to contain two types of mGluR4-immunoreactive axon varicosities: small, weakly immunoreactive varicosities that were distributed randomly (type 1) and large, intensely immunoreactive ones that were often aligned linearly (type 2). In the present study, most type 1 terminals formed asymmetric synapses on dendritic spines, whereas type 2 terminals made symmetric synapses on dendritic shafts, showing immunoreactivity for GABAergic markers. After depletion of neostriatal neurons, type 2 but not type 1 varicosities were largely decreased in the damaged region. When medium-sized spiny neurons (MSNs) were labeled with Sindbis virus expressing membrane-targeted green fluorescent protein, mGluR4 immunoreactivity was observed on some varicosities of their axon collaterals in immunofluorescence and immunoelectron microscopies. Furthermore, type 2 varicosities were often positive for substance P but mostly negative for striatal interneuron markers and preproenkephalin. Thus, striatonigral/striato-entopeduncular MSNs are likely to be the largest source of type 2 mGluR4-immunopositive axon terminals in the neostriatum. Next, in the double-immunofluorescence study, almost all choline acetyltransferase (ChAT)-immunopositive and 41% of NK1 receptor-positive dendrites were heavily associated with type 2 mGluR4-immunoreactive varicosities. Neuronal nitric oxide synthase (nNOS)-positive dendrites, in contrast, seemed associated with only a few type 2 varicosities. Conversely, almost all type 2 varicosities were closely apposed to NK1 receptor-positive dendrites that were known to be derived from cholinergic and nNOS-producing interneurons. These findings indicate that the mGluR4-positive terminals of MSN axon collaterals selectively form synapses with neostriatal cholinergic interneurons.

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Section: Kainic Acid Injection Into the Neostriatummentioning

confidence: 52%

Metabotropic glutamate receptor 4‐immunopositive terminals of medium‐sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum

Kuramoto

Fujiyama

Unzai

et al. 2006

J of Comparative Neurology

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“…2b′′,c′′; VGluT2, data not shown). This indicated that most afferents to the neostriatum were preserved in the damaged region, although some changes would have occurred in the afferent terminals (Wuerthele et al, 1978; Krammer et al, 1979). Thus, the present findings suggested that type 1 mGluR4‐positive excitatory terminals were of cortical or thalamic origin.…”

Section: Resultsmentioning

confidence: 99%

Studies of the central nervous system‐derived CAD cell line, a suitable model for intraneuronal transport studies?

Hou

Aktiv

et al. 2007

J of Neuroscience Research

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The CAD cell line is a variant of a CNS-derived Cath.a cell line established by targeted oncogenesis in transgenic mice. Cell differentiation of the cell line can be induced by "starvation," i.e., removal of serum from the culture medium (protein-free medium). The differentiated CAD cells extend long processes, which ultrastructurally contain abundant microtubules, intermediate filaments, and various synaptic vesicles/organelles in the varicose enlargements, thus resembling neurites. Histochemical studies demonstrated that the differentiated cells express a number of synaptic vesicle proteins, cytoskeletons, different neurotransmitter enzymes, neuropeptides, and glia proteins. The data suggest that the differentiated CAD cells may be a suitable model for studies of intraneuronal transport, recycling of receptors, and pharmacological investigations.

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“…Kainic acid has been used successfully to destroy dLGN neurons in a variety of species, including cat (Ferster & Lindstrom, 1985), rat (Peterson & Moore, 1980;Woodward & Coull, 1982, 1988Carpenter et al, 1986), bushbaby (Fitzpatrick & Diamond, 1979) and tree shrew (Horn & Carey, 1987). However, the selectivity of kainic acid for cell bodies and dendrites has been questioned, since there is evidence for changes in afferent terminal structure and function following kainic acid injection (Wuerthele et al, 1978;Meibach et al, 1978;Krammer et al, 1979;Mason & Fibiger, 1979;Horn & Carey, 1987;Bonanno et al, 1989). For the purposes of our model, it is essential that we establish that kainic acid 03004864/91 $03.00 +.12 9 1991 Chapman and Hall Ltd.…”

Section: Introductionmentioning

confidence: 94%

Selectivity of kainic acid as a neurotoxin within the dorsal lateral geniculate nucleus of the cat: a model for transneuronal retrograde degeneration

1991

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In situ injections of the cytotoxin kainic acid were used to make localized lesions of the dorsal lateral geniculate nucleus in the adult cat to produce a model for studying the effects of postsynaptic target loss. Kainic acid has been used extensively to produce lesions of neuronal cell bodies within the central nervous system. However, the selectivity of kainic acid has been questioned, as it may also affect afferent terminals or axons of passage. Retinal projections to degenerated geniculate nuclei were visualized 1 week after kainate injection using anterograde labelling with horseradish peroxidase and electron microscopy. The results demonstrate the presence of afferent terminals within regions of neuronal loss, and hence the selectivity of kainic acid for intrinsic geniculate neurons.

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Is there a selectivity of neuronal degeneration induced by intrastriatal injection of kainic acid?

Cited by 40 publications

References 20 publications

Metabotropic glutamate receptor 4‐immunopositive terminals of medium‐sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum

Metabotropic glutamate receptor 4‐immunopositive terminals of medium‐sized spiny neurons selectively form synapses with cholinergic interneurons in the rat neostriatum

Studies of the central nervous system‐derived CAD cell line, a suitable model for intraneuronal transport studies?

Selectivity of kainic acid as a neurotoxin within the dorsal lateral geniculate nucleus of the cat: a model for transneuronal retrograde degeneration

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