Is There a Right Way to Interpret SPY? Normalization of Indocyanine Green Angiography Readings in a Burn Model

Disulfiram reduces metastatic osteosarcoma tumor burden in an immunocompetent Balb/c or-thotopic mouse model

“…There was a significant difference in primary tumor burden—normalized quantitative indocyanine green (ICG) hindlimb fluorescence [ 14 , 15 ]—between groups (Figure 1 , Table 2 ). High-resolution ICG measurements were unavailable in 14/120 mice, as noted in Table 2 .…”

“…While we have previously validated our methodology for measuring primary tumor and metastatic OS burden utilizing quantitative ICG angiography [ 14 , 15 ], our model has limitations. First, previous work has noted that the current safe and effective dose of 5mg/kg of ICG used in this study may not be a high enough concentration to detect small micro-metastasis [ 44 ].…”

Reply to the Letter to the Editor: The Chitranjan Ranawat Award: Running Subcuticular Closure Enables the Most Robust Perfusion After TKA: A Randomized Clinical Trial

“…While the authors address a variety of issues they believe to be imperfections in our methodology, we caution that a more-careful read would reveal that our execution was quite-often synchronous with their suggestions. They note previously published work from their respective institutions [1][2][3][4] has demonstrated that measuring the maximum quantitative perfusion is the most-reliable method for normalizing a variety of potentially confounding host variables. Furthermore, they assert any effort to the contrary would represent an unnecessary ''reinventing of the wheel.''…”

Letter to the Editor: The Chitranjan Ranawat Award: Running Subcuticular Closure Enables the Most Robust Perfusion After TKA: A Randomized Clinical Trial

“…While intravenous injection makes the delivery of ICG somewhat more consistent between patients, it does not adequately control for metabolism and tissue thickness. Our group previously proposed using the maximum quantitative blood flow measured in normal tissue within the field of view as a common normalization point [1]. For any points or regions of interest highlighted within an active field, the maximum normal tissue value adjusts for delivery variance, permitting multifield and between-sample comparisons.…”

“…Clinical Orthopaedics and Related Research 1 editors and board members are on file with the publication and can be viewed on request. The opinions expressed are those of the writers, and do not reflect the opinion or policy of CORR1 or The Association of Bone and Joint Surgeons 1 .…”