Is there a relation between clinical disease activity and acute phase response in Behcet's disease?

Melikoğlu, Mehmet; Topkarcı, Zeynep

doi:10.1111/ijd.12224

Cited by 22 publications

(18 citation statements)

References 15 publications

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“…Arginine levels showed a significant correlation with CRP. ESR and CRP levels have been the most widely used laboratory assessments in the routine clinical practice as indicators of BD activation [15].…”

Section: Resultsmentioning

confidence: 99%

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Asymmetric dimethylarginine levels in Behçet's Disease

Öztürk¹,

Vatansev²,

Taner³

et al. 2014

Turk J Bioch

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Objective: Behçet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods:The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging. and myocardial infarction; (c) diabetes mellitus, renal and hepatic diseases, active infectious diseases; (d) other known chronic inflammatory diseases. The body mass index (BMI) and blood pressure were recorded. It was also noted that whether the subjects smoke or not. Each subject was evaluated in active and inactive disease periods. At the time of examination, the presence of two or more of the following Behçet's clinical features was considered as active disease: Oral ulceration, genital ulceration, skin lesions, ocular lesions, active major vessel disease, and active major organ involvement including active gastrointestinal or neurological lesions. Subjects with one lesion or lesion-free period were regarded in inactive disease period.The protocol was approved by the Ethics Committee of the Selcuklu Medical Faculty (24.06.2010Faculty (24.06. -2010) and all subjects volunteered for the trial and written consent was obtained according to the Declaration of Helsinki.Blood samples were centrifuged with 2000 x g for 10 min at +4°C, followed by serum removal for assays. All serum samples were stored at -80°C until analysis.Measurements of ADMA, arginine and citrulline levels were accomplished by high performance liquid chromatography (HPLC), using the method described by Taner et al [17]. In brief, 20 mg 5-sulfosalisilic acid was added to 1 ml serum, and the mixture was left in an ice bath for 10 min. The precipitated protein was removed by centrifugation at 2,000×g for 10 min. Ten µl of the supernatant was filtered using a 0.45-μm pore size filter, which was then mixed with 100 μl derivatization reagent [prepared by dissolving ...

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Section: Resultsmentioning

confidence: 99%

“…Currently, erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are the most widely used laboratory parameters as acute phase response in clinical practice for exacerbation of BD [15].…”

Section: Introductionmentioning

confidence: 99%

Asymmetric dimethylarginine levels in Behçet's Disease

Öztürk¹,

Vatansev²,

Taner³

et al. 2014

Turk J Bioch

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“…10,11 There is no well-established laboratory marker known to be correlated well with BD activity. 12 The aim of this study was to find any association of the BD activity with serum and tear IL-2 levels. To our knowledge, association of BD activity with tear IL-2 levels was not investigated previously.…”

mentioning

confidence: 99%

Association of Disease Activity with Serum and Tear IL-2 Levels in Behçet Disease

Türkçüoğlu

Arat

Kan

et al. 2015

Ocular Immunology and Inflammation

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“…In this study on BD patients, only anti‐lysozyme was associated with patient index scores of BDCAF among five autoantibodies, and it was not correlated with the rest of the autoantibodies. Furthermore, the correlation coefficient of anti‐lysozyme on patient index scores was higher than those of ESR and CRP levels, which had shown good correlation with BDCAF in a previous study . So far, there have been several studies regarding B cells in BD: the number of memory B cells and immunoglobulin G (IgG) secreting B cells, but not the total number of B cells, in BD patients was enhanced, compared to healthy controls, whereas, the number of CD5 + B cell subsets in patients with active BD was not higher than healthy controls .…”

Section: Discussionmentioning

confidence: 73%

“…15,16 ESR and CRP levels are often used for evaluating BD activity, but they are not specific for BD and they can be affected by various pathologic and physiologic conditions. 18 Thus, compared to other diseases, the assessment of BD activity relatively depends upon subjective patients' self-reports and clinicians' examinations. In these clinical situations, we directed our attention to the role of neutrophil and its degranulation in affected tissues in BD pathogenesis.…”

Section: Discussionmentioning

confidence: 99%