Is there a correlation between membrane phospholipid metabolism and cell division?

Michel, G.; Karibian, Doris; Bonnaveiro, N; Stárka, J.

doi:10.1016/s0769-2609(85)80031-4

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…A similar phenotype has been reported for other mutants with pleiotropic envelope defects, including lpxC (envA) point mutants (35,36), mutants lacking components of the twin arginine transport pathway (44), and the murein hydrolase EnvC mutant (28,40). Like Lud135/BC201, the envC mutant PM61 did display intriguing alterations in phospholipid composition (28,29), so perhaps the cell division defect seen here is secondary to the observed phospholipid alterations. Unlike these mutants, Lud135/BC201 is not hypersensitive to detergents or antibiotics, indicating the presence of an intact OM, at least under permissive growth conditions.…”

Section: ϫ10supporting

confidence: 82%

Temperature Sensitivity and Cell Division Defects in an Escherichia coli Strain with Mutations in yghB and yqjA , Encoding Related and Conserved Inner Membrane Proteins

Thompkins¹,

Chattopadhyay²,

Xiao

et al. 2008

J Bacteriol

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Ludox density gradients were used to enrich for Escherichia coli mutants with conditional growth defects and alterations in membrane composition. A temperature-sensitive mutant named Lud135 was isolated with mutations in two related, nonessential genes: yghB and yqjA. yghB harbors a single missense mutation (G203D) and yqjA contains a nonsense mutation (W92TGA) in Lud135. Both mutations are required for the temperature-sensitive phenotype: targeted deletion of both genes in a wild-type background results in a strain with a similar phenotype and expression of either gene from a plasmid restores growth at elevated temperatures. The mutant has altered membrane phospholipid levels, with elevated levels of acidic phospholipids, when grown under permissive conditions. Growth of Lud135 under nonpermissive conditions is restored by the presence of millimolar concentrations of divalent cations Ca 2؉ , Ba 2؉ , Sr 2؉ , or Mg 2؉ or 300 to 500 mM NaCl but not 400 mM sucrose. Microscopic analysis of Lud135 demonstrates a dramatic defect at a late stage of cell division when cells are grown under permissive conditions. yghB and yqjA belong to the conserved and widely distributed dedA gene family, for which no function has been reported. The two open reading frames encode predicted polytopic inner membrane proteins with 61% amino acid identity. It is likely that YghB and YqjA play redundant but critical roles in membrane biology that are essential for completion of cell division in E. coli.

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Section: ϫ10supporting

confidence: 82%

Temperature Sensitivity and Cell Division Defects in an Escherichia coli Strain with Mutations in yghB and yqjA , Encoding Related and Conserved Inner Membrane Proteins

Thompkins¹,

Chattopadhyay²,

Xiao

et al. 2008

J Bacteriol

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“…DHA is known to have effects on cell growth and proliferation ( 28 ). The increased DHA incorporation into the phospholipids of the nucleus membrane may produce a direct influence on modulation of genes, RNA synthesis and release, and the activation of DNA polymerase α, all that can affect cell proliferation ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

“…The main fate of dietary DHA in cells is to incorporate into membrane phospholipids ( 25 – 27 ). DHA can regulate basic membrane properties such as fluidity, permeability and protein activity ( 26 ), thus affecting cell growth and proliferation ( 28 ). The first gap (G1) phase is a period between nuclear division and DNA synthesis, during which various signals derived from metabolism, stress and environmental factors are integrated to determine the developmental program including cell division, differentiation or death ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

DHA Suppresses Hepatic Lipid Accumulation via Cyclin D1 in Zebrafish

et al. 2022

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With the widespread use of high-fat diets (HFDs) in aquaculture, fatty livers are frequently observed in many fish species. The aim of this study was to investigate if docosahexaenoic acid (DHA) could be used to reduce the fatty liver in zebrafish generated by a 16% soybean oil-HFD over 2 weeks of feeding. The DHA was added to iso-lipidic HFD at 0.5, 1.0, and 2.0% of diet. Supplementation of DHA reduced growth and feed efficiency in a dose dependent manner being lowest in the HFDHA2.0 group. Hepatic triglyceride (TG) in zebrafish fed 0.5% DHA-supplemented HFD (HFDHA0.5) was significantly lower than in the HFD control. Transcriptional analyses of hepatic genes showed that lipid synthesis was reduced, while fatty acid β-oxidation was increased in the HFDHA0.5 group. Furthermore, the expression of Cyclin D1 in liver of zebrafish fed HFDHA0.5 was significantly reduced compared to that in fish fed HFD. In zebrafish liver cells, Cyclin D1 knockdown and blocking of Cyclin D1-CDK4 signal led to inhibited lipid biosynthesis and elevated lipid β-oxidation. Besides, DHA-supplemented diet resulted in a rich of Proteobacteria and Actinobacteriota in gut microbiota, which promoted lipid β-oxidation but did not alter the expression of Cyclin D1 in germ-free zebrafish model. In conclusion, DHA not only inhibits hepatic lipid synthesis and promotes lipid β-oxidation via Cyclin D1 inhibition, but also facilitates lipid β-oxidation via gut microbiota. This study reveals the lipid-lowering effects of DHA and highlights the importance of fatty acid composition when formulating fish HFD.

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“…Membrane domains have long been proposed to regulate the bacterial cell cycle [ 99 , 100 , 101 ] and there is now considerable evidence for domains enriched in cardiolipin at the poles and between segregating chromosomes [ 2 , 90 , 102 , 103 ]. There is also complementary evidence for the existence of domains of high microviscosity, resulting from transertion, around the chromosomes [ 104 , 105 ] and even for an increase during the cell cycle in the level of cardiolipin and other lipids [ 104 , 105 , 106 , 107 ]. In the context of Dualism, cardiolipin-rich, fluid domains might correspond to equilibrium structures such that their accumulation would correspond to quantity sensing .…”

Section: The Specific Case Of Dnaa In the E Coli mentioning

confidence: 99%

Chromosome Replication in Escherichia coli: Life on the Scales

Norris

Amar

2012

Life

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At all levels of Life, systems evolve on the 'scales of equilibria'. At the level of bacteria, the individual cell must favor one of two opposing strategies and either take risks to grow or avoid risks to survive. It has been proposed in the Dualism hypothesis that the growth and survival strategies depend on non-equilibrium and equilibrium hyperstructures, respectively. It has been further proposed that the cell cycle itself is the way cells manage to balance the ratios of these types of hyperstructure so as to achieve the compromise solution of living on the two scales. Here, we attempt to re-interpret a major event, the initiation of chromosome replication in Escherichia coli, in the light of scales of equilibria. This entails thinking in terms of hyperstructures as responsible for intensity sensing and quantity sensing and how this sensing might help explain the role of the DnaA protein in initiation of replication. We outline experiments and an automaton approach to the cell cycle that should test and refine the scales concept.

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Is there a correlation between membrane phospholipid metabolism and cell division?

Cited by 7 publications

References 18 publications

Temperature Sensitivity and Cell Division Defects in an Escherichia coli Strain with Mutations in yghB and yqjA , Encoding Related and Conserved Inner Membrane Proteins

Temperature Sensitivity and Cell Division Defects in an Escherichia coli Strain with Mutations in yghB and yqjA , Encoding Related and Conserved Inner Membrane Proteins

DHA Suppresses Hepatic Lipid Accumulation via Cyclin D1 in Zebrafish

Chromosome Replication in Escherichia coli: Life on the Scales

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