Objectives: To evaluate the growth concordance in paired aerobic/ anaerobic bottles, and the impact of the anaerobic growth on patients' antimicrobial management.Method: This is a prospective multicenter study which was conducted in three hospitals, with total beds of 750 beds and 52 ICU beds. Prospectively, laboratory blood cultures logbooks were daily reviewed and patients from whom blood cultures were ordered were followed, their charts were reviewed. Entries on antimicrobial therapeutic changes were noted for all paired bottles. Clinicians were blinded to the study, though they were informed about culture results via the usual work protocol in each hospital.Results: Collected Blood culture bottles totaled 2492; 172 single bottle were excluded, and 1160 paired bottles were analyzed,1046 were concordant; 79 paired bottles had bacterial growth and 967 paired bottles had no bacterial growth. 114 paired bottles were discordant; growth in 97 aerobic bottles, 13 in anaerobic, and 4 in both bottles. The proportion of agreement for the concordant paired growth bottles was 90.2%. The composite proportion of agreement for bottles with any growth (N = 193, composite proportion of agreement = 56%, 95% C.I., 34% -48%). Cohen kappa composite agreement, measured for the total analyzed paired bottles (N = 1160, K= .52, SE = .038. 95% C.I., .447 -.595). The odds of modifying antimicrobial regimen were for total and subgroups intent to treat odds, based on paired bottles showed that one modification took place in one anaerobic growth bottle (N = 1160, Odds = 0.0008), the odds for allThe InTernaTIonal arabIc Journal of anTImIcrobIal agenTs Issn 2174-9094 2015