Is the induction of apoptosis the mechanism of the protective effects of TNFα in helminth infection?

Garside, Paul; Sands, Wiliam A.; Kusel, J. R.; Hagan, Paul

doi:10.1046/j.1365-3024.1996.d01-66.x

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…As shown in Figure 3, with the increasing concentrations of LCL161, the morphological changes indicative of apoptosis in PSCs was more obvious, the apoptotic rate in PSCs was gradually up-regulated, and the transcription levels of both IAP and BIRP genes were also significantly downregulated. Garside (Garside et al, 2010) reported that in helminth infections, caspase, as a TNFα-related factor, can induce the apoptosis in parasites through external signals to hinder their growth. Intriguingly, results of present study also demonstrated that, LCL161-mediated degradation/disruption of the IAPs in caspase-IAPs complexes might have triggered the activity of caspase and result the apoptosis in PSCs, and further studies will be carried out to evaluate the mechanism of Eg-IAPs inhibiting caspase activity in E. granulosus.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Functional Characterization of Inhibitor of Apoptosis Proteins (IAP, BIRP) in Echinococcus granulosus

et al. 2020

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The larval stage of Echinococcus granulosus sensu lato, resulting in cystic echinococcosis, a parasitic zoonosis, causes huge economic losses to the livestock industry and poses a threat to public health. Inhibitor of apoptosis proteins (IAPs) is a class of endogenous anti-apoptotic family, which plays a significant functional role in the regulation of organism's development. Herein, to explore potential functions of IAPs in E. granulosus, two members of IAPs from E. granulosus (Eg-IAP and Eg-BIRP) were cloned, expressed, and molecularly characterized. Eg-IAP and Eg-BIRP encoded putative 331 and 168 residue proteins, respectively. Bioinformatic analysis showed that both proteins contained a type II BIR domain-the essential functional domain of IAPs. Fluorescence immunohistochemistry revealed that both proteins were ubiquitously localized in all life-cycle stages of E. granulosus. Our fluorescent quantitative PCR (RT-qPCR) results revealed relatively higher transcription levels of two Eg-IAPs in protoscoleces (PSCs) compared to the 18-day strobilated worms. We further used different concentrations of LCL161, a Smac-mimetic pan-IAPs inhibitor, to induce the apoptosis in PSCs in vitro, and revealed that the survival rate of PSCs and transcription levels of both genes were negatively correlated with the concentration of LCL161. While the results of light microscopy, transmission electron microscopy (TEM), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay also showed a higher apoptotic rate in PSCs with the increasing concentrations of LCL161. Taken together, our findings provide the reasonable evidence that both Eg-IAP and Eg-BIRP have potential implication in critical anti-apoptotic roles during the development of E. granulosus.

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Section: Discussionmentioning

confidence: 99%

Molecular and Functional Characterization of Inhibitor of Apoptosis Proteins (IAP, BIRP) in Echinococcus granulosus

et al. 2020

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“…They can affect T cells and alter their capacity for proliferation or apoptosis [ 65 ], which affects the balance of the Th1/Th2/Treg response. In addition, some helminths induce apoptosis (i.e., the so-called programmed death) of various immune effector cell populations [ 66 ]. Some helminths affect the level of antibody production by switching the expression of genes encoding IgE antibodies to IgG4 antibodies.…”

Section: Molecular Mechanisms and Immune Response Pathways In Food Al...mentioning

confidence: 99%

Food Allergies and Parasites in Children

Packi,

Rudek,

Matysiak

et al. 2023

Foods

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The dynamically growing incidence of food allergies forces the scientific community to develop new methods for their diagnosis, differentiation, and effective treatment. Parasitoses appear much less frequently in the scientific literature, as well as among the presumed causes of numerous conditions. The similarity of inflammatory mechanisms in allergies and parasitosis necessitates a revision of current diagnostic standards. A lack of specificity and the coincidence of symptoms at an early stage of disease can lead to misdiagnosis. In this paper, we attempted to perform a comparative analysis of the similarities and differences in symptoms for these two types of diseases. We described the molecular mechanisms and metabolic pathways of food allergy and parasitosis. We presented the available research methods and directions of ongoing studies aimed at implementing precise medical techniques for differential diagnosis. We discussed the allergenic properties of certain parasite proteins, using the example of myofibrillar tropomyosins from the nematode Anisakis simplex. The literature in the fields of allergology and parasitology leads to the conclusion that it is reasonable to run parallel allergological and parasitological diagnostics in patients with non-specific symptoms. This approach will facilitate accurate and early diagnosis and implementation of effective therapy.

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Revealing praziquantel molecular targets using mass spectrometry imaging: an expeditious approach applied to Schistosoma mansoni

Ferreira

Oliveira

et al. 2015

International Journal for Parasitology

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Is the induction of apoptosis the mechanism of the protective effects of TNFα in helminth infection?

Cited by 8 publications

References 23 publications

Molecular and Functional Characterization of Inhibitor of Apoptosis Proteins (IAP, BIRP) in Echinococcus granulosus

Molecular and Functional Characterization of Inhibitor of Apoptosis Proteins (IAP, BIRP) in Echinococcus granulosus

Food Allergies and Parasites in Children

Revealing praziquantel molecular targets using mass spectrometry imaging: an expeditious approach applied to Schistosoma mansoni

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