Is the endothelial cell responsible for the thrombus core and shell architecture?

Kadri, Olufemi E.; Surblyte, Migle; Chandran, Vishnu D.; Voronov, Roman S.

doi:10.1016/j.mehy.2019.109244

Cited by 4 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P-selectin is proposed to confer the core its dense compaction and stability against erosion. 16,17 In contrast, the shell consisted of a loose layer of P-selectin-negative platelets with little to no fibrin present. 16,18 The presence of the shell in AIS thrombi has important therapeutic implications.…”

Section: Discussionmentioning

confidence: 99%

“…Three studies utilized microfluidic assays to test their hypotheses and describe the flow conditions under which the shell-core architecture of thrombi arises. [16][17][18] Platelets were labeled with CD41/CD61 (GIIb/IIIa) antibody as well as with an antibody against the transmembrane cell adhesion protein P-selectin (CD62P), which is also expressed by the endothelial cells. SEM Analysis.…”

Section: Methodology Used To Characterize the Mechanisms Of Thrombus ...mentioning

confidence: 99%

“…Moreover, inhibiting the fibrin polymerization did not change thrombus structure or the accumulation of platelets/vWF. Kadri et al 17 described the shell-core structure with the core responsible for attaching the clot to the vessel wall and stopping the blood loss while the shell represents a source of the pathogenic embolism leading to thrombosis and fatal complications. The core and shell of thrombi have a distinct molecular composition due to the limited diffusion of certain larger particles from the core toward the outer parts of thrombi.…”

Section: Hypotheses Of Shell Formationmentioning

confidence: 99%

See 2 more Smart Citations

Shell Versus Core Architecture and Biology of Thrombi in Acute Ischemic Stroke: A Systematic Review

Mereuta,

Agarwal,

Ghozy

et al. 2023

Clin Appl Thromb Hemost

View full text Add to dashboard Cite

Background The presence of an outer shell has been recently described as a common feature of acute ischemic stroke (AIS) thrombi. We performed a systematic review of the current literature on shell genesis, structure, and clinical significance. Methods Following PRISMA guidelines, we searched Ovid Cochrane Central Register of Controlled Trials, Embase, Medline, Scopus, and Web of Science for studies reporting the composition and structure of AIS thrombi and clot analogs. Identified studies were added to Covidence software for primary screening. Two reviewers independently screened titles and abstracts followed by full-text screening. Results From 1290 identified studies, 10 were included in this review. Studies using histology/immunohistochemistry/immunofluorescence described fibrin, platelets, von Willebrand factor, and neutrophil extracellular traps as the main components of the shell. Scanning electron microscopy demonstrated a dense, compact fibrin/platelet-rich shell, and a core rich in polyhedrocytes. Microfluidics studies identified highly activated P-selectin-positive platelets and fibrin forming the core while secondary agonists adenosine diphosphate and thromboxane, along with loosely packed P-selectin-negative platelets constituted the shell. Conclusions The composition, compaction, and integrity of the shell may impact thrombolysis and revascularization outcomes. The preponderance of studies supported this conclusion.

show abstract