Is the Bombali virus pathogenic in humans?

Martell, Henry J; Masterson, Stuart G; McGreig, Jake E; Michaelis, Martin; Wass, Mark N.

doi:10.1093/bioinformatics/btz267

Cited by 19 publications

(16 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Potential determinants of Ebolavirus pathogenicity in humans were identified by analyzing the differentially conserved amino acid positions called specificity determining positions (SDPs) between human pathogenic ebolaviruses and the non-pathogenic Reston virus [25]. Recently, this study was extended to include BOMV to assess its pathogenicity to humans [26]. At SDPs, BOMV shared the majority of amino acids (63.25%) with the human pathogenic Ebolaviruses.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

BioLaboro: A bioinformatics system for detecting molecular assay signature erosion and designing new assays in response to emerging and reemerging pathogens

Holland

Negrón

Mitchell

et al. 2020

Preprint

View full text Add to dashboard Cite

22Background: Emerging and reemerging infectious diseases such as the novel Coronavirus 23 disease, COVID-19 and Ebola pose a significant threat to global society and test the public 24 health community's preparedness to rapidly respond to an outbreak with effective diagnostics 25 and therapeutics. Recent advances in next generation sequencing technologies enable rapid 26 generation of pathogen genome sequence data, within 24 hours of obtaining a sample in some 27 instances. With these data, one can quickly evaluate the effectiveness of existing diagnostics and 28 therapeutics using in silico approaches. The propensity of some viruses to rapidly accumulate 29 mutations can lead to the failure of molecular detection assays creating the need for redesigned 30 or newly designed assays. 31Results: Here we describe a bioinformatics system named BioLaboro to identify signature 32 regions in a given pathogen genome, design PCR assays targeting those regions, and then test the 33 PCR assays in silico to determine their sensitivity and specificity. We demonstrate BioLaboro 34 with two use cases: Bombali Ebolavirus (BOMV) and the novel Coronavirus 2019 (SARS-CoV-35 2). For the BOMV, we analyzed 30 currently available real-time reverse transcription-PCR 36 assays against the three available complete genome sequences of BOMV. Only two met our in 37 silico criteria for successful detection and neither had perfect matches to the primer/probe 38 sequences. We designed five new primer sets against BOMV signatures and all had true positive 39 hits to the three BOMV genomes and no false positive hits to any other sequence. Four assays 40 are closely clustered in the nucleoprotein gene and one is located in the glycoprotein gene. 41

show abstract

Section: Introductionmentioning

confidence: 99%

“…However, for two SDPs in viral protein 24 (VP24), which may be critical for the lack of Reston virus human pathogenicity, the BOMV amino acids match those of Reston virus. Thus, BOMV may not be pathogenic in humans [25, 26]. Nonetheless, rRT-PCR assays are important for biosurveillance of BOMV and other potential new variants in the wild.…”

Section: Introductionmentioning

confidence: 99%

BioLaboro: A bioinformatics system for detecting molecular assay signature erosion and designing new assays in response to emerging and reemerging pathogens

Holland

Negrón

Mitchell

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…However, the omeprazole 364 concentrations that interfered with SARS-CoV-2 CPE formation (IC50 34µM) was 365 beyond therapeutic omeprazole plasma concentrations reported to reach about 8µM 366 [Shin & Kim, 2013]. We have recently shown that omeprazole increases the antiviral 367 activity of acyclovir [Michaelis et al, 2019]. Here, we combined both aprotinin and 368 remdesivir with a fixed omeprazole concentration of 8µM, which resulted in further 369 increased activity against CPE formation (aprotinin 2.7-fold, remdesivir 10-fold) 370…”

mentioning

confidence: 98%

SARS-CoV-2 and SARS-CoV differ in their cell tropism and drug sensitivity profiles

Bojkova

McGreig

McLaughlin

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

18 SARS-CoV-2 is a novel coronavirus currently causing a pandemic. We show 19 that the majority of amino acid positions, which differ between SARS-CoV-2 and the 20 closely related SARS-CoV, are differentially conserved suggesting differences in 21 biological behaviour. In agreement, novel cell culture models revealed differences 22between the tropism of SARS-CoV-2 and SARS-CoV. Moreover, cellular ACE2 23(SARS-CoV-2 receptor) and TMPRSS2 (enables virus entry via S protein cleavage) 24 levels did not reliably indicate cell susceptibility to SARS-CoV-2. SARS-CoV-2 and 25 SARS-CoV further differed in their drug sensitivity profiles. Thus, only drug testing 26 using SARS-CoV-2 reliably identifies therapy candidates. Therapeutic concentrations 27 of the approved protease inhibitor aprotinin displayed anti-SARS-CoV-2 activity. The 28 efficacy of aprotinin and of remdesivir (currently under clinical investigation against 29 SARS-CoV-2) were further enhanced by therapeutic concentrations of the proton 30 pump inhibitor omeprazole (aprotinin 2.7-fold, remdesivir 10-fold). Hence, our study 31 has also identified anti-SARS-CoV-2 therapy candidates that can be readily tested in 32 patients. 33 34

show abstract

“…In contrast, Reston virus (RESTV) does not cause disease in humans but does cause serious, often lethal disease in NHPs, specifically cynomolgus macaques (Macaca fascicularis) [4]. Although studies have demonstrated that the glycoprotein (GP) of the recently identified Bombali virus (BOMV) is capable of mediating entry into human cells, it is not yet known whether BOMV can cause disease in humans [5][6][7]. Lastly, there has been only a single reported case of Taï Forest virus (TAFV) in humans, which occurred in 1994 in Côte-d'Ivoire when a 34-year-old veterinarian became ill after performing an autopsy on a wild chimpanzee found in the Taï Forest [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Taï Forest Virus Does Not Cause Lethal Disease in Ferrets

Schiffman

Yan

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Filoviruses are zoonotic, negative-sense RNA viruses, most of which are capable of causing severe disease in humans and nonhuman primates, often with high case fatality rates. Among these viruses, those belonging to the Ebolavirus genus—particularly Ebola virus, Sudan virus, and Bundibugyo virus—represent some of the most pathogenic to humans. Taï Forest virus (TAFV) is thought to be among the least pathogenic ebolaviruses; however, only a single non-fatal case has been documented in humans, in 1994. With the recent success of the ferret as a lethal model for a number of ebolaviruses, we set out to evaluate its suitability as a model for TAFV. Our results demonstrate that, unlike other ebolaviruses, TAFV infection in ferrets does not result in lethal disease. None of the intramuscularly inoculated animals demonstrated any overt signs of disease, whereas the intranasally inoculated animals exhibited mild to moderate weight loss during the early stage of infection but recovered quickly. Low levels of viral RNA were detected in the blood and tissues of several animals, particularly the intranasally inoculated animals, and all animals mounted a humoral immune response, with high titers of GP-specific IgG detectable as early as 14 days post-infection. These data provide additional insight into the pathogenesis of TAFV.

show abstract

Is the Bombali virus pathogenic in humans?

Cited by 19 publications

References 17 publications

BioLaboro: A bioinformatics system for detecting molecular assay signature erosion and designing new assays in response to emerging and reemerging pathogens

BioLaboro: A bioinformatics system for detecting molecular assay signature erosion and designing new assays in response to emerging and reemerging pathogens

SARS-CoV-2 and SARS-CoV differ in their cell tropism and drug sensitivity profiles

Taï Forest Virus Does Not Cause Lethal Disease in Ferrets

Contact Info

Product

Resources

About