Is Shigella an under-recognized pathogen? A case of pyogenic cervical spondylitis caused by Escherichia coli and Shigella flexneri infection

Zhang, Jiafeng; Liu, Yaoting; Wu, Hongkun; Zhou, Lin

doi:10.1016/j.idcr.2024.e01930

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…It can be divided into four serum groups: Shigella flexneri, Shigella dysenteriae, Shigella boydii, and Shigella sonnei [46]. The strain of Shigella flexneri screened in this study is a major epidemic pathogen in China, which can lead to invasive infection [47]. The above eight pathogenic bacteria screened from the Songhua River water body pose a great threat to human health.…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution and Seasonal Variation of Antibiotic-Resistant Bacteria in an Urban River in Northeast China

Xiao,

Wang,

et al. 2024

Water

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As the largest freshwater river flowing through Harbin, the Songhua River is a standby water source. It is very important to know the species and distribution of antibiotic-resistant bacteria (ARB) in the river. In this study, five antibiotics were selected to screen and identify ARB in spring and autumn. The results showed that the concentration of cefotaxime-resistant bacteria was the highest, and the maximum concentration at S6 in spring was up to 1.40 × 104 CFU/mL. In spring and autumn, bacteria resistant to three antibiotics were screened at S1 of the Songhua River, and bacteria resistant to five antibiotics were screened at S6. No multiple antibiotic-resistant bacteria (MARB) were screened in the other four sites in autumn, while MARB were screened in the other three samples except S2 in spring. In all sample areas in spring and autumn, the probability of screening MARB at S1 and S6 was the highest, reaching 100%. The identification results of 16S rDNA polymerase chain reaction (PCR) products of ARB showed that a total of 51 ARB strains from 15 bacterial genera were screened in the Songhua River, of which 20 ARB strains were from Pseudomonas. Among the 15 bacterial genera, bacteria from 8 bacterial genera have pathogenicity. The results of this study revealed the concentration, spatial distribution, and seasonal variation of culturable ARB in the Songhua River, providing data support for the remediation of antibiotic resistance gene (ARG) pollution in the river.

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Section: Discussionmentioning

confidence: 99%

Spatial Distribution and Seasonal Variation of Antibiotic-Resistant Bacteria in an Urban River in Northeast China

Xiao,

Wang,

et al. 2024

Water

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Characterization of salt brine sulfated polysaccharides: immunomodulatory activity based on gut microbiota

Jia,

Ma,

Xiao

et al. 2024

Blue Biotechnology

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A sulfated polysaccharide consisting of two components with molecular weights of 439 kDa and 16 kDa was extracted from the salt brine. The structural properties, immunomodulatory activity, in vitro fermentation behaviors, and effects of SP on regulating the gut microbiota were investigated. The chemical composition and monosaccharide composition analysis showed that the neutral sugar, protein, uronic acid, and sulfated group contents of SP were 60.42 ± 0.04%, 2.90 ± 0.01%, 13.34 ± 0.01% and 10.51 ± 0.01%, respectively, containing arabinose, galactose, glucose, rhamnose, xylose, mannose, and glucuronic acid in a molar ratio of 33.24:19.18:16.64:13.25:8.31:4.11:5.27. Results from the macrophage cell model showed that SP intervention improved the proliferation activity, phagocytosis of neutral red, and production of IL-6 and TNF-α in RAW 264.7. Furthermore, in vitro fermentation of SP by gut microbiota showed that SCFA production in all treatment groups was significantly higher than that of the blank control group after 48 h of fermentation, especially butyric acid which was 1.70 folds that of the control group. Moreover, long-term fermentation (48 h) of SP improved the diversity of microbiota, decreased the F/B ratio (30.75 at 0 h vs. 1.22 at 48 h), and promoted the growth of probiotics (Parabacteroides, Bacteroidetes, Ruminococcaceae, and Phascolarctobacterium). The positive regulatory effect of SP on the gut microbiota and its metabolites is considered a potential target for its immunomodulatory activity. Graphical abstract

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Is Shigella an under-recognized pathogen? A case of pyogenic cervical spondylitis caused by Escherichia coli and Shigella flexneri infection

Cited by 2 publications

References 8 publications

Spatial Distribution and Seasonal Variation of Antibiotic-Resistant Bacteria in an Urban River in Northeast China

Spatial Distribution and Seasonal Variation of Antibiotic-Resistant Bacteria in an Urban River in Northeast China

Characterization of salt brine sulfated polysaccharides: immunomodulatory activity based on gut microbiota

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