Is routine omentectomy of grossly normal omentum helpful in surgery for ovarian cancer? A look at the tumor microenvironment and its clinical implications

Bilbao, Michelle; Ostrovsky, Olga

doi:10.1016/j.ygyno.2020.12.033

Cited by 24 publications

(17 citation statements)

References 55 publications

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“…Patients with OC in the middle and late stages will eventually experience systemic metastasis and gradually die. 25 Thence, it is indispensable to find a target for therapeutics of metastasis to extend the life of OC patients. Our findings indicate that the active performance of GBAS is positively related to the lymph node metastasis of OC patients, and encourages the proliferation and invasion of OC cells.…”

Section: Discussionmentioning

confidence: 99%

GBAS Regulates the Proliferation and Metastasis of Ovarian Cancer Cells by Combining with eEF1A1

et al. 2022

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Background The glioblastoma-amplified sequence (GBAS) is a newly identified gene that is amplified in approximately 40% of glioblastomas. This article probes into the expression, prognostic significance, and possible pathways of GBAS in ovarian cancer (OC). Method Immunohistochemical methods were used to evaluate the expression level of GBAS in OC and its relationship with clinicopathological characteristics and prognosis. Glioblastoma-amplified sequence shRNA was designed to transfect into OC cell lines to silence GBAS expression, then detect the proliferation, apoptosis, and migration ability of the cell. Furthermore, an in vitro tumor formation experiment in mice was constructed to prove the effect of GBAS expression on the growth of OC in vivo. To further study the regulation mechanism of GBAS, we performed co-immunoprecipitation (Co-IP) and shotgun LC-MS mass spectrometry identification. Results Immunohistochemistry indicated that GBAS was markedly overexpressed in OC compared with normal ovarian tissue and was associated with lymph node metastasis. Inhibition of GBAS expression can significantly reduce OC cell proliferation, colony formation, promote cell apoptosis, and reduce the ability of cell migration and invasion. In vivo tumor formation experiments showed that the size and weight of tumors in mice after GBAS expression knockdown was significantly smaller. Glioblastoma-amplified sequence may be combined with elongation factor 1 alpha 1 (eEF1A1) to achieve its regulation in OC. Bioinformatics analysis data indicate that GBAS may be a key regulator of mitochondria-associated pathways, therefore controlling cancer progression. MicroRNA-27b, MicroRNA-23a, and MicroRNA-590 may directly targeting GBAS affects the biological behavior of OC cells. Conclusion The glioblastoma-amplified sequence may regulate the proliferation and metastasis of OC cells by combining with eEF1A1.

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Section: Discussionmentioning

confidence: 99%

GBAS Regulates the Proliferation and Metastasis of Ovarian Cancer Cells by Combining with eEF1A1

et al. 2022

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“…Every year, more than 240,000 women are diagnosed with OV, responsible for 150,000 deaths (55). The vast majority of ovarian cancer fatalities are attributable to the chemoresistant and widely metastatic disease in the late stage (56). Worse more, while the majority of patients will respond to first-line chemotherapy, disease recurrence rates remain high and the 5-year survival rate is extremely low (57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

Molecular subtypes, clinical significance, and tumor immune landscape of angiogenesis-related genes in ovarian cancer

et al. 2022

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Angiogenesis is a physiological process, where new blood vessels are formed from pre-existing vessels through the mechanism called sprouting. It plays a significant role in supporting tumor growth and is expected to provide novel therapeutic ideas for treating tumors that are resistant to conventional therapies. We investigated the expression pattern of angiogenesis-related genes (ARGs) in ovarian cancer (OV) from public databases, in which the patients could be classified into two differential ARG clusters. It was observed that patients in ARGcluster B would have a better prognosis but lower immune cell infiltration levels in the tumor microenvironment. Then ARG score was computed based on differentially expressed genes via cox analysis, which exhibited a strong correlation to copy number variation, immunophenoscore, tumor mutation load, and chemosensitivity. In addition, according to the median risk score, patients were separated into two risk subgroups, of which the low-risk group had a better prognosis, increased immunogenicity, and stronger immunotherapy efficacy. Furthermore, we constructed a prognostic nomogram and demonstrated its predictive value. These findings help us better understand the role of ARGs in OV and offer new perspectives for clinical prognosis and personalized treatment.

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“…Therefore, the ability of cells to contact each other and to adhere to the peritoneum when ovarian cancer cells are suspended is critical for metastasis of ovarian cancer cells. Extensive metastasis is one of the key factors leading to the death of ovarian cancer ( Bilbao et al, 2021 ). Our research found that multiple glycoproteins associated with cell adhesion were differentially expressed in ovarian epithelial cancer SKOV3 cells vs. non-cancerous ovarian epithelial IOSE80 cells, including VTN, VCAM1, ALCAM, CNTN5, PTK7, LAMC1, RGMB, THBS1, DSCAML1, PCDHA6, CD6, and UMOD ( Supplementary Table S6-7 ), and participated in cell adhesion-related signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Comparative glycoproteomics study on the surface of SKOV3 versus IOSE80 cell lines

Zhou

Cai

et al. 2022

Front. Chem.

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Objective: Site- and structure-specific quantitative N-glycoproteomics study of differential cell-surface N-glycosylation of ovarian cancer SKOV3 cells with the non-cancerous ovarian epithelial IOSE80 cells as the control.Methods: C18-RPLC-MS/MS (HCD with stepped normalized collision energies) was used to analyze the 1: 1 mixture of labeled intact N-glycopeptides from SKOV3 and IOSE80 cells, and the site- and structure-specific intact N-glycopeptide search engine GPSeeker was used to conduct qualitative and quantitative search on the obtained raw datasets.Results: With the control of the spectrum-level false discovery rate ≤1%, 13,822 glycopeptide spectral matches coming from 2,918 N-glycoproteins with comprehensive N-glycosite and N-glycan structure information were identified; 3,733 N-glycosites and 3,754 N-glycan sequence structures were confirmed by site-determining and structure-diagnostic fragment ions, respectively. With the control of no less than two observations among the three technical replicates, fold change ≥1.5, and p-value ≤ 0.05, 746 DEPGs in SKOV3 cells relative to IOSE80 cells were quantified, where 421 were upregulated and 325 downregulated.Conclusion: Differential cell-surface N-glycosylation of ovarian cancer SKOV3 cells were quantitatively analyzed by isotopic labeling and site- and structure-specific N-glycoproteomics. This discovery study provides putative N-glycoprotein biomarker candidates for future validation study using multiple reaction monitoring and biochemical methods.

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Is routine omentectomy of grossly normal omentum helpful in surgery for ovarian cancer? A look at the tumor microenvironment and its clinical implications

Cited by 24 publications

References 55 publications

GBAS Regulates the Proliferation and Metastasis of Ovarian Cancer Cells by Combining with eEF1A1

GBAS Regulates the Proliferation and Metastasis of Ovarian Cancer Cells by Combining with eEF1A1

Molecular subtypes, clinical significance, and tumor immune landscape of angiogenesis-related genes in ovarian cancer

Comparative glycoproteomics study on the surface of SKOV3 versus IOSE80 cell lines

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