Is Rodent Virus Contamination of Monoclonal Antibody Preparations for Use in Human Therapy a Hazard?

Carthew, P.

doi:10.1099/0022-1317-67-6-963

Cited by 22 publications

(4 citation statements)

References 78 publications

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“… ■ This method is not suitable for producing antibodies against small peptides and fragment antigens. ■ Hybridoma culture suffers from a high risk of contamination [ 10 , 18 ]. ■ This system of antibody production is now developed for only mice and rats and researchers are working continuously to develop antibodies of human origin [ 19 ].…”

Section: Main Textmentioning

confidence: 99%

“…Retroviruses are a common incidence within mammalian chromosomes. Generally, animals like mice that are used in the production of monoclonal antibodies could carry many viruses such as viscus virus, retrovirus, reovirus, herpes virus, and thymic virus, leading to cross-contamination or infection in humans [ 18 ]. This poses a major threat for cross disease transfer from mice or rats to the human.…”

Section: Main Textmentioning

confidence: 99%

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Hybridoma technology; advancements, clinical significance, and future aspects

Mitra

Tomar

2021

Journal of Genetic Engineering and Biotechnology

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Background Hybridoma technology is one of the most common methods used to produce monoclonal antibodies. In this process, antibody-producing B lymphocytes are isolated from mice after immunizing the mice with specific antigen and are fused with immortal myeloma cell lines to form hybrid cells, called hybridoma cell lines. These hybridoma cells are cultured in a lab to produce monoclonal antibodies, against a specific antigen. This can be achieved by an in vivo or an in vitro method. It is preferred above all the available methods to produce monoclonal antibodies because antibodies thus produced are of high purity and are highly sensitive and specific. Main body of the abstract Monoclonal antibodies are useful in diagnostic, imaging, and therapeutic purposes and have a very high clinical significance. Once hybridoma cells become stable, these cell lines offer limitless production of homogenized antibodies. This method is also cost-effective. The antibodies produced by this method are highly sensitive and specific to the targeted antigen. It is an important tool used in various fields of research such as in toxicology, animal biotechnology, medicine, pharmacology, cell, and molecular biology. Monoclonal antibodies are used extensively in the diagnosis and therapeutic applications. Radiolabeled monoclonal antibodies are used as probes to detect tumor antigens in the living system; also radioisotope coupled antibodies are used for therapeutic target specific action on oncogenic cells. Short conclusion Presently, the monoclonal antibodies used are either raised in mice or rats; this poses a risk of disease transfer from mice to humans. There is no guarantee that antibodies thus created are entirely virus-free, despite the purification process. Also, there are some immunogenic responses observed against the antibodies of mice origin. Technologically advanced techniques such as genetic engineering helped in reducing some of these limitations. Advanced methods are under development to make lab-produced monoclonal antibodies as human as possible. This review discusses the advantages and challenges associated with monoclonal antibody production, also enlightens the advancement, clinical significance, and future aspects of this technique.

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Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Hybridoma technology; advancements, clinical significance, and future aspects

Mitra

Tomar

2021

Journal of Genetic Engineering and Biotechnology

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“…The potential hazards of murine viral contamination of mab preparations intended for therapeutic use were well reviewed recently by Carthew (1986) and are clearly significant. This problem is particularly acute when large-scale production includes the use of ascites preparations induced in either mice or rats.…”

Section: Murine Hybridomasmentioning

confidence: 99%

Viral contamination of monoclonal antibody preparations: Potential problems and possible solutions

Harbour

Woodhouse

1990

Cytotechnology

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“…Rodent viruses are known to contaminate transplantable tumors and other biological materials (2,4,17,19), and some are associated with human disease and death (2,(4)(5)(6)11). The importance of rodent virus contamination of biological products has assumed a new dimension as a result of the preparation in mice of monoclonal antibodies destined for use in humans (3). Guidelines for safety testing are being developed (10) and include the use of the mouse antibody production (MAP) test to detect conventional rodent virus contamination.…”

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confidence: 99%

Comparison of isolation in cell culture with conventional and modified mouse antibody production tests for detection of murine viruses

Souza

Smith

1989

J Clin Microbiol

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The sensitivity of the mouse antibody production test with intraperitoneal or intrasplenic inoculation of mice with reovirus type 3, minute virus of mice, lymphocytic choriomeningitis virus, or mouse hepatitis virus was compared with that of direct isolation in cultured cells. The mouse antibody production test for detection of mouse hepatitis virus was significantly more sensitive than virus isolation in permissive cells, but differences in sensitivity were less marked for the other three viruses. The intrasplenic route of inoculation did not yield seroconversion that occurred earlier or more consistently than that detected after intraperitoneal inoculation.

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Is Rodent Virus Contamination of Monoclonal Antibody Preparations for Use in Human Therapy a Hazard?

Cited by 22 publications

References 78 publications

Hybridoma technology; advancements, clinical significance, and future aspects

Hybridoma technology; advancements, clinical significance, and future aspects

Viral contamination of monoclonal antibody preparations: Potential problems and possible solutions

Comparison of isolation in cell culture with conventional and modified mouse antibody production tests for detection of murine viruses

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