Is red blood cell rheology preserved during routine blood bank storage?

Henkelman, Sandra; Dijkstra‐Tiekstra, Margriet J.; Wildt‐Eggen, J. de; Graaff, Reindert; Rakhorst, Gerhard; Oeveren, Willem van

doi:10.1111/j.1537-2995.2009.02521.x

Cited by 65 publications

(68 citation statements)

References 39 publications

(96 reference statements)

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“…The ATP content of the red cells does not, therefore, reliably reflect red cell deformability at approved storage times. Similar results have been reported by others for LD red cell concentrates resuspended in saline adenine glucose mannitol (SAGM) [19]. The results of our study are in accordance with previous studies investigating deformability during storage.…”

Section: Correlation Analyses (Table 4)supporting

confidence: 83%

“…Henkelman et al [19] also used LORCA to study the aggregability and deformability in LD red cell concentrates in SAGM over a storage period of 7 weeks. In contrast to our study, aggregability did not drop steadily during storage, probably due to the low number of units investigated (n = 10), resulting in unstable estimates of the parameter values.…”

Section: Discussionmentioning

confidence: 99%

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Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Karger

Lukow

Kretschmer³

2012

Transfus Med Hemother

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KeywordsATP content · Deformability · Quality · Red cell storage · Storage lesion · In vivo survival · Whole blood Summary Background: Storage duration of red cells has been associated with increased morbidity and mortality following transfusion. This association has been attributed to the loss of deformability of stored red cells leading to deterioration of microvascular perfusion. ATP content is considered a critical determinant of the deformability of stored red cells. Methods: ATP content and deformability were determined after storage for up to 49 days in 40 leukocyte-depleted whole blood units. Red cell deformability was determined using a laser-assisted optical rotational cell analyzer (LORCA ® ) employing shear stress (SS) ranging from 0.3 to 30 Pa. Deformability was expressed as the elongation index (EI). EI was correlated with ATP content. Results: ATP content decreased from 3.5 to 1.7 mol/g hemoglobin. EI increased from 0.03 to 0.05 at an SS of 0.

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Section: Correlation Analyses (Table 4)supporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Karger

Lukow

Kretschmer³

2012

Transfus Med Hemother

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“…RBCs over the storage process have been mechanically characterized in both stretching and folding modes, and different metrics for indicating RBC deformability were used 17,18,48 . The elongation index (EI) and deformation index (DI) were phenomenologically defined and are strongly dependent on the flow velocity.…”

Section: Rbc Effective Stiffness Increases During Storagementioning

confidence: 99%

“…More recently, ektacytometry was used to characterize the deformability of stored RBCs by stretching RBCs with shear stress induced by a rotating plate. The extent of RBC elongation, defined as the elongation index (EI), was used to indicate deformability under a certain shear stress 16,17 . A microfluidic measurement was also reported for investigating stored RBCs' deformability, wherein the deformation index (DI) did not show significant differences at different storage time points 18 .…”

Section: Introductionmentioning

confidence: 99%

Stiffness increase of red blood cells during storage

Zheng

Wang

et al. 2018

Microsyst Nanoeng

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In transfusion medicine, the deformability of stored red blood cells (RBCs) changes during storage in blood banks. Compromised RBC deformability can reduce the transfusion efficiency or intensify transfusion complications, such as sepsis. This paper reports the microfluidic mechanical measurement of stored RBCs under the physiological deformation mode (that is, folding). Instead of using phenomenological metrics of deformation or elongation indices (DI or EI), the effective stiffness of RBCs, a flow velocityindependent parameter, is defined and used for the first time to evaluate the mechanical degradation of RBCs during storage. Fresh RBCs and RBCs stored up to 6 weeks (42 days) in the blood bank were measured, revealing that the effective stiffness of RBCs increases over the storage process. RBCs stored for 1 week started to show significantly higher stiffness than fresh RBCs, and stored RBC stiffness degraded faster during the last 3 weeks than during the first 3 weeks. Furthermore, the results indicate that the time points of the effective stiffness increase coincide well with the degradation patterns of S-nitrosothiols (SNO) and adenosine triphosphate (ATP) in RBC storage lesions.

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“…These alterations of stored RBCs, known as storage lesions, are primarily divided into three types: biochemical, morphological, and structural [1,2]. During storage, RBC alterations may result in their easy disruption during transfusion, which could be harmful to the transfusion recipient [1,3,4,5]. In addition, previous studies demonstrate that RBC alterations may be of crucial importance for the maintenance of normal circulation [6,7,8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Dysregulation Associated with Red Blood Cell Storage

Chen

Xie

Xing

et al. 2018

Transfus Med Hemother

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Introduction: Stored red blood cells (RBCs) undergo storage lesions involving morphological, physiological and biochemical changes. MicroRNAs (miRNAs) have important functions in cell apoptosis and life processes. Therefore, the aim of this study was to explore potential roles of miRNAs in the damage of stored RBCs. Methods: Blood samples were collected from 13 healthy male O-type donors, and leuko-reduced RBCs were divided into fresh RBC group and 20-day storage RBC group. Results: Eight predicted miRNAs with modified expressions with an intersection ≥ 3 were found dysregulated in the 20-day storage RBC group and involved in apoptosis and senescence signaling pathway: miR-31-5p, miR-196a-5p, miR-203a, miR-654-3p and miR-769-3p were increased, while miR-96-5P, miR-150-5P and miR-197-3p were decreased. Evidence associating miR-31-5p, miR-203a, miR-654 and miR-769 to RBCs or blood in general are not available. Conclusions: Dysregulated miRNAs might represent potential biomarkers to identify storage lesions, and their detection might help to evaluate the quality of stored RBCs.

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Is red blood cell rheology preserved during routine blood bank storage?

Abstract: RBC biochemical and physical alterations during storage minimally affected the RBC ability to aggregate and deform, even after prolonged storage. The rheologic properties of leukoreduced RBC units were well preserved during 7 weeks of routine blood bank storage.

Cited by 65 publications

References 39 publications

Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Stiffness increase of red blood cells during storage

MicroRNA Dysregulation Associated with Red Blood Cell Storage

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