Is Platelet-Derived Growth Factor-BB Expression Proportional to Fibrosis in the Hypertrophied Lumber Ligamentum Flavum?

Zhang, Yu; Chen, Jianting; Zhong, Zhaoming; Yang, Dehong; Zhu, Qingan

doi:10.1097/brs.0b013e3181f3d2df

Cited by 42 publications

(48 citation statements)

References 33 publications

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“…TGF-β has been documented to play a significant part for the hypertrophy of LF [18]. Zhang and coworkers reported that the increased expression of platelet-derived growth factor-BB was observed in the hypertrophic lumbar LF [19]. Furthermore, connective tissue growth factor expression was upregulated and correlated with the LF thickness [20].…”

Section: Discussionmentioning

confidence: 99%

Hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis is associated with increased bFGF expression

Honsawek

Poonpukdee

Chalermpanpipat

et al. 2013

International Orthopaedics (SICOT)

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Purpose A prospective study was undertaken to investigate basic fibroblast growth factor (bFGF) expression in hypertrophic ligamentum flavum (LF) from patients with lumbar spinal canal stenosis (LSCS) and to determine whether there was a correlation of bFGF expression with LF thickness. Methods Twenty patients with lumbar spinal canal stenosis were enrolled in this study. bFGF mRNA and protein expressions in LF were analyzed using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), respectively. The thickness of LF was measured by axial T1-weighted magnetic resonance imaging. Results Expression of bFGF was substantially higher in the hypertrophic LF group than in the control group (P<0.001) as quantified by quantitative real-time PCR. In immunohistochemical study, bFGF was positively stained on the fibroblasts within hypertrophic LF compared to nonpathologic LF of controls. Subsequent ELISA analysis revealed that bFGF concentration in the hypertrophic LF group was remarkably higher than that in the control group (P=0.003). The thickness of LF in the hypertrophic LF was significantly greater than that in the control group (P<0.001). LSCS patients with greater severity of LF hypertrophy had significantly higher bFGF levels in the LF tissues (P<0.001). Furthermore, the bFGF concentration exhibited a positive correlation with the LF thickness (r=0.974, P<0.001). Conclusions These findings suggest that the increased expression of bFGF is associated with the hypertrophy of ligamentum flavum in patients with LSCS.

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Section: Discussionmentioning

confidence: 99%

Hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis is associated with increased bFGF expression

Honsawek

Poonpukdee

Chalermpanpipat

et al. 2013

International Orthopaedics (SICOT)

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“…6 Removal of the thickened ligamentum fl avum is a main target in the surgical treatment of LSCS. 10 Some authors have evaluated the thickness of the ligamentum fl avum using MRI or computerized tomography and indicated that the ligamentum fl avum is signifi cantly thicker in LSCS. 8-10 , 16-18 Some authors have argued that the ligamentum fl avum thickening is due to tissue hypertrophy, but others have attributed it to the buckling of the ligamentum fl avum.…”

Section: Discussionmentioning

confidence: 99%

Chondrogenic and Fibrotic Process in the Ligamentum Flavum of Patients With Lumbar Spinal Canal Stenosis

Yabe

Hagiwara

Andõ

et al. 2015

Spine

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“…In recent years, an increased expression of TGF-β1 has been demonstrated in hypertrophied LF 7 8. Zhang et al 9 reported high platelet-derived growth factor BB (PDGF-BB) expression in pathological lumbar LF. Previous studies have shown fibrosis-like chondroid metaplasia of ligament fibroblasts and calcium pyrophosphate crystal deposition in the hypertrophied LF of patients with LSCS 6 10.…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of Vascular Endothelial Growth Factor is Associated with Hypertrophic Ligamentum Flavum in Lumbar Spinal Canal Stenosis

Jirathanathornnukul

Limthongkul

Yingsakmongkol

et al. 2016

Journal of Investigative Medicine

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Lumbar spinal canal stenosis (LSCS) is the most common spinal disorder in elderly patients, causing low back and leg pain, radiculopathy, and cauda equina syndrome. Vascular endothelial growth factor (VEGF) is a potent regulator of many cellular functions including proliferation, differentiation, wound healing, and angiogenesis. The present study aimed to investigate the pattern of VEGF expression in the ligamentum flavum (LF) of patients with LSCS. 24 patients with LSCS were recruited in this prospective study. We quantified and localized VEGF expression in LF tissues obtained during surgery. VEGF messenger RNA and protein expression in LF were determined using reverse transcription PCR (RT-PCR), and quantitative real-time PCR, immunohistochemistry, and ELISA. VEGF expression was significantly higher in the hypertrophic LF group than in the non-pathological LF group (p<0.01) as quantified by quantitative real-time PCR. Further ELISA analysis showed that the average concentration of VEGF in the hypertrophic LF was significantly elevated compared with that of controls (p<0.01). There was no correlation between the tissue VEGF expression of non-pathological LF and patient age in patients with LSCS. Moreover, the immunohistochemical study revealed that VEGF was positively stained on fibroblasts, inflammatory cells, and endothelial cells representing neovascularization within hypertrophic LF compared to the non-pathological LF of controls. The increased expression of VEGF was associated with the degenerative changes of hypertrophic LF, suggesting that VEGF could contribute to one of the mechanisms of pathogenesis in lumbar spinal stenosis.

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Is Platelet-Derived Growth Factor-BB Expression Proportional to Fibrosis in the Hypertrophied Lumber Ligamentum Flavum?

Abstract: A higher PDGF-BB expression existed in the hypertrophied LF of patients with LSS and could be a risk factor of the fibrosis.

Cited by 42 publications

References 33 publications

Hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis is associated with increased bFGF expression

Hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis is associated with increased bFGF expression

Chondrogenic and Fibrotic Process in the Ligamentum Flavum of Patients With Lumbar Spinal Canal Stenosis

Increased Expression of Vascular Endothelial Growth Factor is Associated with Hypertrophic Ligamentum Flavum in Lumbar Spinal Canal Stenosis

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