Is persistent polyclonal b lymphocytosis caused by epstein‐barr virus? A study with polymerase chain reaction and in situ hybridization

Chow, Kuan‐Chih; Witzig, Thomas E.; Li, Chin‐Yang ‐Y; Nacilla, Johnny Q.

doi:10.1002/ajh.2830410409

Cited by 44 publications

(19 citation statements)

References 19 publications

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“…25 Similarly, a specific fragment of the LMP-1 gene was present in all seven PPBL patients but undetected in PBMC of normal subjects. Our recent data and others [16][17][18] strongly support the presence of a latent EBV infection in patients with PPBL. HLA-DR7, present in most PPBL patients, might potentially play a role in facilitating viral infection.…”

Section: Discussionsupporting

confidence: 83%

“…Because EBV has also been identified in cells of patients with PPBL, 16,17 we hypothesized that our patient might have an EBV-related lymphoma. Several genes are believed to play a critical role for the immortalizing function of EBV; of these, EBNA-1 is essential for viral replication and always expressed in NHL associated with EBV.…”

Section: Resultsmentioning

confidence: 99%

“…15 Other findings incriminating EBV include reports in which viral DNA has been amplified by PCR and identified in lymphoid cells obtained from two patients with PPBL using in situ hybridiz-ation. 16,17 An EBV-positive line has also been established from another patient with PPBL. 18 The potential for malignancy in PPBL remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Large cell lymphoma complicating persistent polyclonal B cell lymphocytosis

et al. 1998

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Persistent polyclonal B cell lymphocytosis (PPBL) is a rare lymphoproliferative disorder of unclear natural history and its potential for B cell malignancy remains unknown. We describe the case of a 39-year-old female who presented with stage IV-B large cell lymphoma 19 years after an initial diagnosis of PPBL; her disease was rapidly fatal despite intensive chemotherapy and blood stem cell transplantation. Because we had recently identified multiple bcl-2/lg gene rearrangements in blood mononuclear cells of patients with PPBL, we sought evidence of this oncogene in this particular patient: bcl-2/lg gene rearrangements were found in blood mononuclear cells but not in lymphoma cells. Owing to the possible role of Epstein-Barr virus (EBV) in the pathogenesis of PPBL, we also hypothesized our patient might have an EBV-related lymphoproliferative disorder. Despite serologies consistent with past exposure to this virus, it was not found in lymphoma cells using a sensitive polymerase chain reaction technique. We conclude that nonHodgkin's lymphoma may occur during the course of PPBL. However, longer follow-up in more patients will be needed in order to better clarify the risk of hematologic malignancy in patients with PPBL.

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Section: Discussionsupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

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Large cell lymphoma complicating persistent polyclonal B cell lymphocytosis

et al. 1998

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“…Evidence of past EBV infection is more common than in the general population, and Chow et al (1992) demonstrated the presence of EBV DNA in the abnormal lymphocytes of an affected patient. The association with cigarette smoking is equally strong, and in one reported case the lymphocytosis resolved on stopping and returned on restarting smoking (Carstairs et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

Persistent polyclonal B‐cell lymphocytosis in identical twins

1997

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Summary. This is the first report of the unusual syndrome of persistent polyclonal B-cell lymphocytosis occurring in monozygotic twins. The syndrome is characterized by a lymphocytosis, with circulating atypical, binucleated lymphocytes, mild splenomegaly and raised serum IgM. It occurs predominantly in females, with serological evidence of previous EBV infection, and is associated with cigarette smoking and HLA-DR7 phenotype. The association with DR7 suggests a genetic predisposition. Its occurrence in identical twins, documented here, provides stronger support for a hereditary/genetic basis for the syndrome.

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“…A non-isotopic method, using a mixture of fluorescein isothiocyanate (FITC)-labelled IL-6 antisense oligonucleotides, was used to determine the expression of IL-6 mRNA in cell lines and pathological sections (Chow et al, 1992). The probe sequences were (Wang et al, 1988): 5′-GGACAGGTTTCTGACCAGA-AGAA GGAATGCCC-3′ (IL-6 mRNA, 750-719) 5′-ACT-GCAGGAACTCCTTAAAGCTGCGCAGAA-3′ (IL-6 mRNA, 660-631) 5′-TCACCAGGCAAGTCTCCTCATTGAATCCAG-3′ (IL-6 mRNA, 390-361).…”

Section: Ishmentioning

confidence: 99%

Expression and up-regulation of interleukin-6 in oesophageal carcinoma cells by n-sodium butyrate

Wang

Chow

1999

Br J Cancer

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Recently, the serum level of interleukin (IL)-6 has been shown to correlate with disease progression and prognosis of cancer patients. However, the available information about the source and the pathophysiological regulation of IL-6 in cancer cells is limited. Thus, in this study, we tried to identify the source and the clinical roles of serum IL-6 in patients with oesophageal squamous cell carcinoma (ESCC), and then further to characterize the biological regulation of IL-6 in ESCC cell lines. Sera and tissue specimens from 80 consecutive patients with ESCC were collected between 1993 and 1997. Additionally, three ESCC cell lines were used for in vitro study. The concentration of serum IL-6 was measured by enzyme-linked immunosorbent assay (ELISA), and correlated the survival time with measured IL-6 level. Expressions of IL-6, IL-6Rα (IL-6 receptor alpha) and gp130 in pathological sections and cell lines were characterized by immunological staining. Detection of IL-6 mRNA was determined by in situ hybridization (ISH) and reverse transcription-polymerase chain reaction (RT-PCR). Up-regulation of IL-6 by n-sodium butyrate (n-BT) was studied in ESCC cell lines. The levels of serum IL-6 in patients with ESCC were significantly higher than those in the healthy controls. Serum levels of IL-6 were also shown to correlate with disease progression and survival. However, sCD8 levels and lymphocyte counts in the peripheral blood were not parallel to the changed pattern of serum IL-6. In pathological sections and ESCC cell lines, message of IL-6 was identified by ISH in cancer cells. Expression of IL-6 mRNA was further confirmed with RT-PCR in ESCC cell lines. Although IL-6 was detected in some ESCC cell lines, IL-6 gene expression and protein production could be induced or enhanced by n-BT treatment in all three cell lines. The serum levels of IL-6 are frequently elevated at diagnosis of ESCC, and are associated with poor prognosis. IL-6 that could be produced by cancer cells is up-regulated by n-BT. © 1999 Cancer Research Campaign

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Is persistent polyclonal b lymphocytosis caused by epstein‐barr virus? A study with polymerase chain reaction and in situ hybridization

Cited by 44 publications

References 19 publications

Large cell lymphoma complicating persistent polyclonal B cell lymphocytosis

Large cell lymphoma complicating persistent polyclonal B cell lymphocytosis

Persistent polyclonal B‐cell lymphocytosis in identical twins

Expression and up-regulation of interleukin-6 in oesophageal carcinoma cells by n-sodium butyrate

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