Is oxidative stress involved in the sex-dependent response to ochratoxin A renal toxicity?

Enciso, José Manuel; Ceráin, Adela López de; Pastor, Laura; Azqueta, Amaya; Vettorazzi, Ariane

doi:10.1016/j.fct.2018.04.050

Cited by 14 publications

(11 citation statements)

References 62 publications

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“…In addition, we found non-significant variations in CRE levels across all the groups due to the fact that OTA mainly affects the renal tubular reabsorption function, but has little effect on glomerular filtration function, and further research is needed to confirm this [42]. There is increasing evidence that OTA causes its toxic effects via oxidative stress [43]. In contrast, after treatment with both ASX and OTA, the levels of the above-mentioned biomarkers were restored towards control levels.…”

Section: Discussionmentioning

confidence: 75%

Protective Effect of Astaxanthin on Ochratoxin A-Induced Kidney Injury to Mice by Regulating Oxidative Stress-Related NRF2/KEAP1 Pathway

et al. 2020

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The present study aimed to investigate the effects of astaxanthin (ASX) on ochratoxin A (OTA)-induced renal oxidative stress and its mechanism of action. Serum kidney markers, histomorphology, ultrastructural observation, and oxidative stress indicators were assessed. Meanwhile, quantitative real-time reverse transcription PCR and western blotting detection of NRF2 (encoding nuclear factor, erythroid 2 like) and members of the NRF2/KEAP1 signaling pathway (KEAP1 (encoding Kelch-like ECH-associated protein), NQO1 (encoding NAD(P)H quinone dehydrogenase), HO-1 (encoding heme oxygenase 1), γ-GCS (gamma-glutamylcysteine synthetase), and GSH-Px (glutathione peroxidase 1)) were performed. Compared with the control group, the OTA-treated group showed significantly increased levels of serum UA (uric acid) and BUN (blood urea nitrogen), tubular epithelial cells were swollen and degenerated, and the levels of antioxidant enzymes decreased significantly, and the expression of NRF2 (cytoplasm), NQO1, HO-1, γ-GCS, and GSH-Px decreased significantly. More importantly, after ASX pretreatment, compared with the OTA group, serum markers were decreased, epithelial cells appeared normal; the expression of antioxidant enzymes increased significantly, NQO1, HO-1, γ-GCS and GSH-Px levels increased significantly, and ASX promoted the transfer of NRF2 from the cytoplasm to the nucleus. These results highlight the protective ability of ASX in renal injury caused by OTA exposure, and provide theoretical support for ASX’s role in other mycotoxin-induced damage.

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Section: Discussionmentioning

confidence: 75%

Protective Effect of Astaxanthin on Ochratoxin A-Induced Kidney Injury to Mice by Regulating Oxidative Stress-Related NRF2/KEAP1 Pathway

et al. 2020

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“…However, our results demonstrated an equivalent sensitivity for HaCaT cells lysed for a minimum of 30 min at room temperature or overnight lysis at 4 °C, which saves a considerable amount of time. After a comparison between the effect of H 2 O 2 , Ro19–8022, and X-rays on different cell types, Enciso et al showed that the effect of lysis duration depends on the damaging agent, and therefore the type(s) of DNA damage under examination [29,35]. OECD guidelines also emphasize the critical influence of lysis conditions for the conversion of alkali-labile lesions to strand breaks [16,35].…”

Section: Discussionmentioning

confidence: 99%

“…After a comparison between the effect of H 2 O 2 , Ro19–8022, and X-rays on different cell types, Enciso et al showed that the effect of lysis duration depends on the damaging agent, and therefore the type(s) of DNA damage under examination [29,35]. OECD guidelines also emphasize the critical influence of lysis conditions for the conversion of alkali-labile lesions to strand breaks [16,35]. Furthermore, cell type-specific differences [36] may need to be taken into consideration when optimal lysis conditions are evaluated.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Major Steps in the Conventional Protocol for the Alkaline Comet Assay

Karbaschi

Abdulwahed

et al. 2019

IJMS

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Single cell gel electrophoresis, also known as the comet assay, has become a widespread DNA damage assessment tool due to its sensitivity, adaptability, low cost, ease of use, and reliability. Despite these benefits, this assay has shortcomings, such as long assay running time, the manipulation of multiple slides, individually, through numerous process steps, the challenge of working in a darkened environment, and reportedly considerable inter- and intra-laboratory variation. All researchers typically perform the comet assay based upon a common core approach; however, it appears that some steps in this core have little proven basis, and may exist, partly, out of convenience, or dogma. The aim of this study was to critically re-evaluate key steps in the comet assay, using our laboratory’s protocol as a model, firstly to understand the scientific basis for why certain steps in the protocol are performed in a particular manner, and secondly to simplify the assay, and decrease the cost and run time. Here, the shelf life of the lysis and neutralization buffers, the effect of temperature and incubation period during the lysis step, the necessity for drying the slides between the electrophoresis and staining step, and the need to perform the sample workup and electrophoresis steps under subdued light were all evaluated.

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“…The nephrotoxic effect of the toxin has been demonstrated by various studies performed on laboratory animals [2,35]. Severe nephrotoxicity has been detected in rats [113], with renal damages characterized by disorganization of the tubules, apoptosis, polyploidy in the proximal convo-luted tubule (PCT) and an increase in the nucleus-cytoplasm ratio of their kidneys [114][115][116][117]. Furthermore, tubular nephrosis and hemorrhage were detected in rat kidneys [118].…”

Section: Toxicity Of Otamentioning

confidence: 99%

Ochratoxin A in Slaughtered Pigs and Pork Products

Vlachou

Pexara

Σολωμάκος

et al. 2022

Toxins

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Ochratoxin A (OTA) is a mycotoxin that is produced after the growth of several Aspergillus and Penicillium spp. in feeds or foods. OTA has been proved to possess nephrotoxic, hepatotoxic, teratogenic, neurotoxic, genotoxic, carcinogenic and immunotoxic effects in animals and humans. OTA has been classified as possibly carcinogenic to humans (Group 2B) by the IARC in 2016. OTA can be mainly found in animals as a result of indirect transmission from naturally contaminated feed. OTA found in feed can also contaminate pigs and produced pork products. Additionally, the presence of OTA in pork meat products could be derived from the direct growth of OTA-producing fungi or the addition of contaminated materials such as contaminated spices. Studies accomplished in various countries have revealed that pork meat and pork meat products are important sources of chronic dietary exposure to OTA in humans. Various levels of OTA have been found in pork meat from slaughtered pigs in many countries, while OTA levels were particularly high in the blood serum and kidneys of pigs. Pork products made from pig blood or organs such as the kidney or liver have been often found to becontaminated with OTA. The European Union (EU) has established maximum levels (ML) for OTA in a variety of foods since 2006, but not for meat or pork products. However, the establishement of an ML for OTA in pork meat and meat by-products is necessary to protect human health.

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Is oxidative stress involved in the sex-dependent response to ochratoxin A renal toxicity?

Cited by 14 publications

References 62 publications

Protective Effect of Astaxanthin on Ochratoxin A-Induced Kidney Injury to Mice by Regulating Oxidative Stress-Related NRF2/KEAP1 Pathway

Protective Effect of Astaxanthin on Ochratoxin A-Induced Kidney Injury to Mice by Regulating Oxidative Stress-Related NRF2/KEAP1 Pathway

Evaluation of the Major Steps in the Conventional Protocol for the Alkaline Comet Assay

Ochratoxin A in Slaughtered Pigs and Pork Products

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