Is multiplexed off-axis holography for quantitative phase imaging more spatial bandwidth-efficient than on-axis holography? [Invited]

Dardikman, Gili; Shaked, Natan T.

doi:10.1364/josaa.36.0000a1

Cited by 28 publications

(25 citation statements)

References 43 publications

(107 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Splitting the dynamic range of the camera between the multiplexed holograms could possibly cause reduction in the signal to noise ratio, yet this effect is practically negligible for phase images of mostly transparent samples such as isolated biological cells in a watery medium. 32 …”

Section: Discussionmentioning

confidence: 99%

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

2022

Self Cite

View full text Add to dashboard Cite

Three-dimensional (3D) optical imaging of rapidly moving biological cells is difficult to achieve as such samples cannot be scanned over time. Here, we present a dynamic scan-free optical tomography approach for stain-free 3D imaging of biological cells using our new double six-pack tomography technique, whereby 12 off-axis holograms are captured in a single camera exposure without sacrificing resolution or field of view. The proposed system illuminates the sample from 12 angles simultaneously, and 3D refractive index (RI) tomograms are reconstructed from each recorded video frame of the dynamic sample. The technique is verified experimentally by recording flowing silica beads, 3 μm in diameter, with the resulting tomogram RI accuracy being 98.5%. A live swimming sperm cell is also imaged, and dynamic 3D imaging results for both beads and sperm cell are presented. The proposed technique represents a 12-fold increase in dynamic holographic data for tomography.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The space-bandwidth efficiency can be defined by the available SBP per measurement with a given pixel amount. 52 Our prototype setup has not been optimized for extreme space-bandwidth efficiency. The non-reconstructable FS components occupy a large part of the detector bandwidth.…”

Section: ■ Discussionmentioning

confidence: 99%

Single-Shot High-Throughput Phase Imaging with Multibeam Array Interferometric Microscopy

et al. 2021

View full text Add to dashboard Cite

High-throughput microscopic imaging is highly desirable in biomedical applications. Advances in computational microscopy have achieved high space-bandwidth products and even permitted gigapixel imaging in a stepwise fashion, yet temporal resolution remains challenging for investigating live-sample dynamics. Here, we report multibeam array interferometric microscopy (MAIM) for a single-shot high space-bandwidth product. The MAIM method overcomes the limitations of conventional digital holographic microscopy, providing complex field reconstruction with a maximum 5-fold field of view (FOV) increase in a single camera acquisition, while maintaining sub-nanometer optical path-length stability. This is achieved by integrating common-path holographic microscopy, multibeam interference technology, and holographic multiplexing technology. The temporal resolving power of MAIM is significantly higher than that of computational illumination microscopy. MAIM has major advantages over previous holographic multiplexing techniques in that it integrates more wavefronts and offers high temporal stability. The fundamentals of MAIM are analyzed theoretically. As a demonstration, we build MAIM prototypes to increase the FOV by factors of 5, 4, and 3, respectively. We present proof-of-concept MAIM imaging results of both natural and artificial samples and show biomedical applications such as monitoring sub-cellular dynamical phenomena in flowing live erythrocytes in vitro and label-free microrefractometry imaging of unstained cancer tissue slices. MAIM gives rise to (ultra)fast or long-term (time-lapse) imaging of nanoscale dynamics of unstained live samples in vitro with a high throughput.

show abstract

“…Although it is easy to experimentally verify this condition, a rule of thumb to aid in the design can be extracted. As we know that the first order experiences a shift ksinβ, the condition for separation is k>3∆k/2, where ∆k = 2 π M obj λ NA is the signal bandwidth [48]. Thus, we obtain the condition…”

Section: Configuration Bmentioning

confidence: 92%

Compact off-axis holographic slide microscope: design guidelines

Cacace¹,

Bianco²,

Mandracchia³

et al. 2020

Biomed. Opt. Express

View full text Add to dashboard Cite

Holographic microscopes are emerging as suitable tools for in situ diagnostics and environmental monitoring, providing high-throughput, label-free, quantitative imaging capabilities through small and compact devices. In-line holographic microscopes can be realized at contained costs, trading off complexity in the phase retrieval process and being limited to sparse samples. Here we present a 3D printed, cost effective and field portable off-axis holographic microscope based on the concept of holographic microfluidic slide. Our scheme removes complexity from the reconstruction process, as phase retrieval is non iterative and obtainable by hologram demodulation. The configuration we introduce ensures flexibility in the definition of the optical scheme, exploitable to realize modular devices with different features. We discuss trade-offs and design rules of thumb to follow for developing DH microscopes based on the proposed solution. Using our prototype, we image flowing marine microalgae, polystyrene beads, E.coli bacteria and microplastics. We detail the effect on the performance and costs of each parameter, design, and hardware choice, guiding readers toward the realization of optimized devices that can be employed out of the lab by non-expert users for point of care testing.

show abstract

Is multiplexed off-axis holography for quantitative phase imaging more spatial bandwidth-efficient than on-axis holography? [Invited]

Cited by 28 publications

References 43 publications

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

Dynamic Tomographic Phase Microscopy by Double Six-Pack Holography

Single-Shot High-Throughput Phase Imaging with Multibeam Array Interferometric Microscopy

Compact off-axis holographic slide microscope: design guidelines

Contact Info

Product

Resources

About