Is MLC phosphorylation essential for the recovery from ROCK inhibition in glioma C6 cells?

Korczyński, Jarosław; Sobierajska, Katarzyna; Krzemiński, Patryk; Wasik, A.; Wypych, Dorota; Pomorski, Paweł; Kłopocka, Wanda

doi:10.18388/abp.2011_2295

Cited by 14 publications

(8 citation statements)

References 37 publications

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“…With ROCK inhibition, MLC kinase-activated stress fibers are not assembled in the cell center [86]. The physiological function of actin cables at the periphery of the cell also appeared to differ from that of the central cables [67, 87, 88]. In mouse embryonic fibroblasts, FCs showed the same periodic localization as MLC kinase suggesting that early adhesion-site formation is designated by myosin-dependent retractions [89].…”

Section: Discussionmentioning

confidence: 99%

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Unraveling the Determinants of Protrusion Formation

Varghese

Gorsevski

Cayer

et al. 2012

International Journal of Cell Biology

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A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the cables showed distinctive size and orientation, as indicated by correlation of 7 values with FC shape. Results obtained with myosin inhibitors support the interpretation that a central, isometric, contractile network puts constraints on both factor 5 and 7 protrusions. We conclude that cells establish functional domains by rearranging the cytoskeleton.

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Section: Discussionmentioning

confidence: 99%

“…In smooth muscle cells, ROCK activation stimulated contraction [62], while dominant-acting mutants of ROCK induced stress fibers and focal adhesions [63–66]. Conversely, ROCK inhibition released stress fibers from their attachments to FCs [67]. …”

Section: Introductionmentioning

confidence: 99%

Unraveling the Determinants of Protrusion Formation

Varghese

Gorsevski

Cayer

et al. 2012

International Journal of Cell Biology

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“…The obtained images were converted to 8‐bit tiff images. Next, an axonal length and cellular dimensions (i.e., length and width) were determined using LAS AF software (Leica Microsystems) equipped with all measurement tools . A mean value of eight different measurements was determined and a standard deviation was calculated.…”

Section: Methodsmentioning

confidence: 99%

Assessment of degradation and biocompatibility of electrodeposited chitosan and chitosan–carbon nanotube tubular implants

Nawrotek

Tylman

Decherchi

et al. 2016

J Biomedical Materials Res

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Designing three-dimensional tubular materials made of chitosan is still a challenging task. Availability of such forms is highly desired by tissue engineering, especially peripheral nerve tissue engineering. Aiming at this problem, we use an electrodeposition phenomenon in order to obtain chitosan and chitosan-carbon nanotube hydrogel tubular implants. The in vitro biocompatibility of the fabricated structures is assessed using a mouse hippocampal cell line (mHippoE-18). As both implants do not induce significant cytotoxicity, they are next subjected to in vitro degradation studies in the environment simulating in vivo conditions for specified periods of time: 7, 14, and 28 days. The mass loss of implants indicates their stability at the tested time period; therefore, the materials are subcutaneously implanted in Sprague Dawley rats. The explants are collected after 7, 14, and 28 days. The assessment of composition and changes in tissues surrounding the implanted materials is made in respect to surrounding tissue thickness as well as the number of blood vessels, macrophages, lymphocytes, and neutrophils. No symptoms of acute inflammation are noticed at any point in time. The observed regular healing process allows concluding that both chitosan and chitosan-carbon hydrogel tubular implants are biocompatible with high application potential in tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2701-2711, 2016.

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“…Myosin II activation in these two cable systems is controlled by ROCK and MLCK through MLC phosphorylation. Also, the physiological function of peripheral actin cables in the cell appeared to differ from that of the central cables [193,216,217]. FCs in mouse embryonic fibroblasts showed the same periodic localization as MLCK suggesting that early adhesion-site formation is designated by myosin-dependent retractions [218].…”

Section: Actomyosin Contractility In Factor 7 Feature Formationmentioning

confidence: 97%

“…It has been observed that in smooth muscle cells, ROCK activation stimulated contraction [188], while dominant-acting mutants of ROCK induced stress fibers and focal adhesions [189][190][191][192]. Conversely, ROCK inhibition released stress fibers from their attachments to FCs [193].…”

Section: Fcsmentioning

confidence: 99%

Abstract 5112: To be or not to be a protrusion: Unraveling the determinants of protrusion formation

Varghese¹,

Heckman²,

Boudreau³

et al. 2010

Cancer Research

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Cellular edge features have been widely studied in recent years due to the interest in cell motility in normal and cancerous cells. Cues from cell interactions with the extracellular environment lead to overall cytoskeletal remodeling and consequently shape changes. The factors that determine the shape of a cell include actin polymerization, myosin II contraction and focal contacts (FCs). Actin based structures like lamellipodia (broad protrusions), ruffles (vertical protrusions) and filopodia (elongated, tapering protrusions) are the major protrusion types described in the scientific literature. However, their subjective classification is a problem when trying to understand mechanisms of protrusion formation in different cell types. The present study makes use of a previously determined computerized morphometric classification technique to distinguish features other than lamellipodia in 1000W rat tracheal epithelial cells. Some of the features identified through previous studies were factor 4, factor 5, factor 7 and factor 16. The aim of the present study was to explore the role of some of the structural components leading to the formation of a protrusion, particularly to a feature defined as factor 7. Integrated morphometry analysis with MetaMorph software was used to study the FC characteristics, while a geographical information system (GIS) software was used to study the FC orientation with respect to a protrusive feature. Small FCs favored factor 7 feature formation. High factor 7 values were observed when the class of FCs without actin became narrower. Paradoxically, where anchored actin cables on FCs showed a positive correlation with factor 7, the inner radius and width of the FC were the only elevated variables. The results suggested that certain populations of FCs with actin, which were narrow but varied in orientation, are important vii ACKNOWLEDGEMENTS As I stand on the verge of fulfilling one of my long cherished goals, I cannot help but take a look back and cherish all the abundant blessings and grace showered upon me by the Almighty. Without the support, guidance and friendship of many a people, this journey would have been incomplete. I would like to express my heartfelt gratitude and sincere appreciation to my research advisor, Dr Carol Heckman for providing the opportunity to work with her and for being a supportive yet daunting mentor all through the years of my study. Her guidance, perseverance and above all her kindness has touched me in many ways. She has not only guided my way to becoming a researcher but also encouraged me to tread paths less taken and be an independent thinker. My special acquaintance with her will always be cherished in the years to come. I would like to thank my committee members: Dr. Peter Gorsevski, Dr. Michael Geusz and Dr. Roudabeh Jamasbi for their positivity, encouragement and invaluable inputs during the course of the study. I would specially like to thank Dr Peter Gorsevski for agreeing to serve as a committee member and for introducing me to biospatial statisti...

show abstract

Is MLC phosphorylation essential for the recovery from ROCK inhibition in glioma C6 cells?

Cited by 14 publications

References 37 publications

Unraveling the Determinants of Protrusion Formation

Unraveling the Determinants of Protrusion Formation

Assessment of degradation and biocompatibility of electrodeposited chitosan and chitosan–carbon nanotube tubular implants

Abstract 5112: To be or not to be a protrusion: Unraveling the determinants of protrusion formation

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