Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

Yahyaoui, Emna; Marinoni, Daniela Torello; Botta, Roberto; Ruffa, Paola; Germana, Maria

doi:10.3389/fpls.2021.778142

Cited by 6 publications

(5 citation statements)

References 35 publications

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“…It allows obtaining high-quality micro-plants without the phenomena of necrosis or hyperdigestion, which can be easily acclimatised. The aim of research by Yahyaoui et al (2021) was to carry out sanitary treatment of varieties and clean plants from the most important viral pathogen -the apple mosaic virus. This research makes it possible to create in vitro true and virus-free hazelnut seedlings using apex encapsulation technology.…”

Section: Introductionmentioning

confidence: 99%

“…This research makes it possible to create in vitro true and virus-free hazelnut seedlings using apex encapsulation technology. In addition, the viability, regrowth and conversion rates of both conventional meristem tip cultures (MTC) and unconventional (MTC combined with encapsulation technology) sanitation methods were evaluated (Yahyaoui et al 2021). The studies of Gao et al (2008) were related to the propagation of five genotypes derived from Corylus heterophylla × C. avellana hybrids.…”

Section: Introductionmentioning

confidence: 99%

“…bacteria are most common in hazelnuts. Reed et al (1997) and Yahyaoui et al (2021) believe that persistent bacterial and fungal contamination is a serious problem in microclonal reproduction of the crops. The effect of carbon sources on the propagation of hazelnut shoots was studied by Yu et al (1993).…”

Section: Introductionmentioning

confidence: 99%

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Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Matskevych

Yukhnovskyi

Kimeichuk

et al. 2023

Folia Forestalia Polonica

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The purpose of the research was to identify the physiological–biochemical and anatomical–morphological peculiarities found during in vitro cultivation of Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb, which occur as a result of the action of trophic and phytohormonal determinants. The research was conducted on three hazelnut varieties (Trapezund, Jefferson, Tonda Gentile Romana) and four almond varieties (E5 Borozan, M41 Alex, Georgia, Louise). A theoretical and experimental rationale for their use in the microclonal propagation of these cultures has been developed. The effectiveness of the preparatory stage before introduction into aseptic conditions for growing mother plants in closed soil conditions with scattered artificial lighting and microbiological protection has been proven. This reduced self-intoxication by oxidation products with phenol-like substances and microflora contamination of primary explants. Among the nutrient media compared, the best was Nas and Read (NRM) for hazelnuts and Nas Almond Medium (NAM) for almonds. Passaging on the same media leads to a decrease in regeneration indicators. In regenerants of almonds, the rosette of the shoots was noted, and in hazelnuts, the death of apical buds was also found. To prevent this, alternating NRM and driver and kuniyuki walnut (DKW) media for hazelnuts and NAM and Quirin and Lepoivre (QL) for almonds was effective. Long-term cultivation on media with a high content of synthetic analogues of phytohormones leads to the accumulation of phytotoxic effects with each subsequent passage. The phytotoxic effect of cytokinins was manifested in hyperhydration of shoots and of auxins in callus fertilisation. On comparing the ontogenesis of regenerants from explants isolated from mother plants aged 30–180 days, it was found that the optimal age for hazelnut and almond was 90 days. To reset the trophic and hormonal determinants, the introduction of explant donors into a state of dormancy has been successfully used. At the final fourth stage of microclonal propagation, in order to adapt plants simultaneously with an increase in the number of regenerants, it is effective to use the photoautotrophic method of microclonal propagation with intensive lighting and air enriched with carbon dioxide. For the transition of plants from conventional heterotrophic propagation to autotrophic cultivation, an intermediate stage of pre-adaptation of regenerants in wet chamber conditions is effective.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Matskevych

Yukhnovskyi

Kimeichuk

et al. 2023

Folia Forestalia Polonica

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“…D. opposita ‘Tiegun’ is a popular Chinese yam cultivar that has been commercially grown in Jiaozuo city of Henan province, China for a long time due to its medicinal and nutritional benefits [ 3 ]. However, vegetative propagation of yam through its tubers year after year leads to the spread of viral infections and declining quality [ 4 – 6 ]. Researchers have successfully developed microtuber culture techniques, providing virus-free tubers suitable for transportation and storage [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of BBX gene family and its function in the regulation of microtuber formation in yam

et al. 2023

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BBX proteins play important roles in all of the major light-regulated developmental processes. However, no systematic analysis of BBX gene family regarding the regulation of photoperiodic microtuber formation has been previously performed in yam. In this study, a systematic analysis on the BBX gene family was conducted in three yam species, with the results, indicating that this gene plays a role in regulating photoperiodic microtuber formation. These analyses included identification the BBX gene family in three yam species, their evolutionary relationships, conserved domains, motifs, gene structure, cis-acting elements, and expressional patterns. Based on these analyses, DoBBX2/DoCOL5 and DoBBX8/DoCOL8 showing the most opposite pattern of expression during microtuber formation were selected as candidate genes for further investigation. Gene expression analysis showed DoBBX2/DoCOL5 and DoBBX8/DoCOL8 were highest expressed in leaves and exhibited photoperiod responsive expression patterns. Besides, the overexpression of DoBBX2/DoCOL5 and DoBBX8/DoCOL8 in potato accelerated tuber formation under short-day (SD) conditions, whereas only the overexpression of DoBBX8/DoCOL8 enhanced the accelerating effect of dark conditions on tuber induction. Tuber number was increased in DoBBX8/DoCOL8 overexpressing plants under dark, as well as in DoBBX2/DoCOL5 overexpressing plants under SD. Overall, the data generated in this study may form the basis of future functional characterizations of BBX genes in yam, especially regarding their regulation of microtuber formation via the photoperiodic response pathway.

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“…Virus elimination in vitro plant culture is the most effective approach to obtain virus-free germplasm resources of superior variety to be applied to many crops, such as Solanum tuberosum [ 10 , 11 ]. Virus-free regenerated plants grow fast and have strong disease resistance ability and significantly improved production and quality [ 12 ]. Few regeneration systems have been established through callus genesis for S. miltiorrhiza [ 13 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants

Yao

Jiang

et al. 2022

Plant Methods

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Bcakground The dry root and rhizome of Salvia miltiorrhiza are used to treat cardiovascular diseases, chronic pain, and thoracic obstruction over 2000 years in Asian countries. For high quality, Sichuan Zhongjiang is regarded as the genuine producing area of S. miltiorrhiza. Given its abnormal pollen development, S. miltiorrhiza from Sichuan (S.m.-SC) relies on root reproduction and zymad accumulation; part of diseased plants present typical viral disease symptoms and seed quality degeneration. This study aim to detected unknown viruses from mosaic-diseased plants and establish a highly efficient virus-free regeneration system to recover germplasm properties. Results Tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) were detected from mosaic-diseased plants. Primary apical meristem with two phyllo podium in 0.15–0.5 mm peeled from diseased plants were achieved 73.33% virus-free rate. The results showed that the medium containing MS, 0.5 mg/L 6-BA, 0.1 mg/L NAA, 0.1 mg/L GA3, 30 g/L sucrose and 7.5 g/L agar can achieve embryonic-tissue (apical meristem, petiole and leaf callus) high efficient organogenesis. For callus induction, the optimal condition was detected on the medium containing MS, 2 mg/L TDZ, 0.1 mg/L NAA by using secondary petiole of virus-free plants under 24 h dark/d condition for 21 d. The optimal system for root induction was the nutrient solution with 1/2 MS supplemented with 1 mg/L NAA. After transplant, the detection of agronomic metric and salvianolic acid B content confirmed the great germplasm properties of S.m.-SC virus-free plants. Conclusions A highly efficient virus-free regeneration system of S.m.-SC was established based on the detected viruses to recover superior seed quality. The proposed system laid support to control disease spread, recover good germplasm properties in S.m.-SC.

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Is It Possible to Produce Certified Hazelnut Plant Material in Sicily? Identification and Recovery of Nebrodi Genetic Resources, in vitro Establishment, and Innovative Sanitation Technique From Apple Mosaic Virus

Cited by 6 publications

References 35 publications

Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Peculiarities of determining the morphogenesis of plants Corylus avellana L. and Prunus dulcis (Mill.) D.A.Webb in vitro culture

Identification of BBX gene family and its function in the regulation of microtuber formation in yam

Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants

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