IS
 1R
 -Mediated Plasticity of IncL/M Plasmids Leads to the Insertion of
 bla
 OXA-48
 into the Escherichia coli Chromosome

Beyrouthy, Racha; Robin, Frédéric; Delmas, Julien; Gibold, Lucie; Dalmasso, Guillaume; Dabboussi, Fouad; Hamzé, Monzer; Bonnet, Richard

doi:10.1128/aac.02669-14

Cited by 56 publications

(65 citation statements)

References 31 publications

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“…The genetic structure of bla OXA‐48 in L/M plasmids is different from that of other carbapenemases. This gene is usually part of Tn 1999 (Tn 1999 –Tn 1991.4 ), with Tn 1991.2 being the most prevalent . In 2016, Cuzon and colleagues reported an L/M plasmid carrying GES‐5 and GES‐6 on the same plasmid (Table ).…”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

171

178

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Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities. Resistance to multiple β‐lactams complicates effective clinical management of CRE infections. Using plasmid typing methods, a wide distribution of plasmid replicon groups has been reported in CREs around the world, including IncF, N, X, A/C, L/M, R, P, H, I, and W. We performed a literature search for English research papers, published between 2013 and 2018, reporting on plasmid‐mediated carbapenem resistance. A rise in both carbapenemase types and associated plasmid replicon groups was seen, with China, Canada, and the United States recording a higher increase than other countries. blaKPC was the most prevalent, except in Angola and the Czech Republic, where OXA‐181 (n = 50, 88%) and OXA‐48–like (n = 24, 44%) carbapenemases were most prevalent, respectively; blaKPC‐2/3 accounted for 70% (n = 956) of all reported carbapenemases. IncF plasmids were found to be responsible for disseminating different antibiotic resistance genes worldwide, accounting for almost 40% (n = 254) of plasmid‐borne carbapenemases. blaCTX‐M, blaTEM, blaSHV, blaOXA‐1/9, qnr, and aac‐(6′)‐lb were mostly detected concurrently with carbapenemases. Most reported plasmids were conjugative but not present in multiple countries or species, suggesting limited interspecies and interboundary transmission of a common plasmid. A major limitation to effective characterization of plasmid evolution was the use of PCR‐based instead of whole‐plasmid sequencing–based plasmid typing.

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Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

171

178

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“…Plasmids act as the scaffolds upon which arrays of antibiotic resistance genes are assembled, generating multiple-drug-resistant phenotypes in Enterobacteriaceae. However, only a limited number of CRE have been found to carry carbapenemase genes on their chromosomes (3)(4)(5)(6). Notably, only two strains of Escherichia coli have been reported to carry chromosomally integrated bla NDM-1 in China and India (7,8).…”

mentioning

confidence: 99%

Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried bla _NDM-1

Sakamoto

Akeda

Sugawara

et al. 2018

Antimicrob Agents Chemother

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We report here Klebsiella pneumoniae strains carrying chromosomal blaNDM-1 in Thailand. The genomes of these two isolates include a 160-kbp insertion containing blaNDM-1, which is almost identical to that in the IncHI1B-like plasmid. Further analysis indicated that IS5-mediated intermolecular transposition and Tn3 transposase-mediated homologous recombination resulted in the integration of blaNDM-1 into the chromosome from an IncHI1B-like plasmid. The spread of this type of carbapenem-resistant Enterobacteriaceae may threaten public health and warrants further monitoring.

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“…However, due to the emergence of carbapenem resistance, the antimicrobial activity of these drugs is no longer effective (2). Production of carbapenemases is one of the main mechanisms for carbapenem resistance (3).…”

mentioning

confidence: 99%

Outbreak of OXA-48-Producing Klebsiella pneumoniae Involving a Sequence Type 101 Clone in Batna University Hospital, Algeria

Loucif

Kassah-Laouar

et al. 2016

Antimicrob Agents Chemother

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Seven nonredundant ertapenem-resistant Klebsiella pneumoniae isolates were collected between May 2014 and 19 January 2015 in the nephrology and hematology units of Batna University Hospital in Algeria. All strains coproduced the bla OXA-48 , bla CTX-M-15 , bla SHV-1 , and bla TEM-1D genes. Six of these isolates belonged to the pandemic clone sequence type 101 (ST101). The bla OXA-48 gene was located on a conjugative IncL/M-type plasmid. This is the first known outbreak of OXA-48-producing K. pneumoniae isolates involving an ST101 clone in Batna University Hospital.

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IS 1R -Mediated Plasticity of IncL/M Plasmids Leads to the Insertion of bla _OXA-48 into the Escherichia coli Chromosome

Abstract: The OXA-48 carbapenemase is mainly encoded by ϳ62-kb IncL/M plasmids. However, chromosome-mediated genes have been observed in Escherichia coli isolates. In this work, we investigated the genetic environment of OXA-48 in members of the family Enterobacteriaceae

Cited by 56 publications

References 31 publications

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried bla _NDM-1

Outbreak of OXA-48-Producing Klebsiella pneumoniae Involving a Sequence Type 101 Clone in Batna University Hospital, Algeria

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IS 1R -Mediated Plasticity of IncL/M Plasmids Leads to the Insertion of bla OXA-48 into the Escherichia coli Chromosome

Abstract: The OXA-48 carbapenemase is mainly encoded by ϳ62-kb IncL/M plasmids. However, chromosome-mediated genes have been observed in Escherichia coli isolates. In this work, we investigated the genetic environment of OXA-48 in members of the family Enterobacteriaceae

Cited by 56 publications

References 31 publications

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried bla NDM-1

Outbreak of OXA-48-Producing Klebsiella pneumoniae Involving a Sequence Type 101 Clone in Batna University Hospital, Algeria

Contact Info

Product

Resources

About

IS 1R -Mediated Plasticity of IncL/M Plasmids Leads to the Insertion of bla _OXA-48 into the Escherichia coli Chromosome

Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried bla _NDM-1