Is human DNA enough?—potential for bacterial DNA

Leake, Sarah Louise

doi:10.3389/fgene.2013.00282

Cited by 17 publications

(13 citation statements)

References 10 publications

(12 reference statements)

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“…As pointed out by Gunn et al (2014) and Leake (2013), the forensic interest in DNA goes well beyond the standard approaches to DNA profiling that represent the current stateof-the-art in many contemporary legal systems, and this raises questions as to how new forms of data ought to be dealt with in an operational perspective (Milot et al, 2013). Although these frontiers topics clarify the extent to which there is room for exciting future research in this area, it should not distract us from the fact that even in the current state of forensic practice, there are hurdles and pressing topics that ask for efficient answers.…”

mentioning

confidence: 76%

DNA, statistics and the law: a cross-disciplinary approach to forensic inference

2014

Frontiers Research Topics

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confidence: 76%

DNA, statistics and the law: a cross-disciplinary approach to forensic inference

2014

Frontiers Research Topics

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“…Microbial forensics has become a useful adjunct to investigations of bioterrorism and biocrime [13] and its potential has been recognized as an alternative plan to overcome limitations of current forensic science, such as a deficiency in blood, tissue, semen, or saliva at the site of crime scene investigation. Bacterial DNA analysis will not replace standard DNA identification, but could become a complimentary technique for when standard DNA identification provides only limited information [1]. Bacterial components recovered from computer keyboards following use allows for adequate characterization and comparison of bacterial community, and surfaces that are touched can be linked to individual skin surface [14].…”

Section: Discussionmentioning

confidence: 99%

“…These individuals had not taken antibiotics. This was important as antibiotics, diet, and smoking can influence the microbiome composition [1].…”

Section: Sample Collection and Dna Extractionmentioning

confidence: 99%

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Microbial Forensic Analysis of Bacterial Fingerprint by Sequence Comparison of 16S rRNA Gene

Lee¹,

Woo²,

Choi³

et al. 2015

J Forensic Res

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Human have the microorganisms in the skin, gut and mouth. Human skin microbiome based on the 16S rRNA encoding gene can reveal bacterial species diversity. Also bacterial species in the skin have diverse and unique composition between individuals. We thought that a bacterial fingerprint obtained from surfaces including computer keyboards aids forensic individual identification in case of evidence deficiency. Next generation sequencing was used to analyze the bacterial community on objects and fingertips to match the object to the individual. The 16S rRNA gene sequence was submitted to EMBL SRA with accession number PRJEB8760. Higher similarity of bacterial community between public computer keyboards and laboratory member's fingertips were evident than between other locations including doorknobs. Here we studied the challenges that bacterial fingerprint can be used as a human identification tool in forensic fields.

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“…Skin is the largest organ of human body, and provides ideal habitats for various microorganisms. Among them, hand is one of the parts in direct contact with outer environment, and thus can leave various potential evidences such as fingerprints and micro-evidences at crime scenes [1]. Microorganisms in the hands include bacteria, viruses, fungi and protozoa.…”

Section: Introductionmentioning

confidence: 99%

Microbial forensic analysis of human-associated bacteria inhabiting hand surface

Park

Kim

Lee

et al. 2017

Forensic Science International: Genetics Supplement Series

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Microorganisms are microscopic organisms, either single-celled or multicellular, and their association with humans is well represented by the term human microbiome. Skin is the largest organ of the human body and primary interface with outer environment, and thus harbors various microorganisms. Recent studies recognized the significance of bacterial flora found on the surface of the skin and its application in personal identification. In particular, hand surface can be an environment of primary interest, as it harbors higher level of microbial diversity than other parts of the skin. In this study, diversity of microbial communities inhabiting the palms of different individuals was explored using culture-based methods A total of 686 bacterial strains were obtained, and identified based on 16S rRNA gene sequence analysis. Among the isolates, the genera Staphylococcus, Micrococcus and Enhydrobacter were recovered as major taxa. Twenty strains could be recognized as candidates for novel species, as the 16S rRNA gene sequence similarities with known species were around 97%. Variations in the bacterial composition among individuals could be observed. The resultant data confirmed a high potential of palm microbial flora in the forensic application for personal identification.

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Is human DNA enough?—potential for bacterial DNA

Cited by 17 publications

References 10 publications

DNA, statistics and the law: a cross-disciplinary approach to forensic inference

DNA, statistics and the law: a cross-disciplinary approach to forensic inference

Microbial Forensic Analysis of Bacterial Fingerprint by Sequence Comparison of 16S rRNA Gene

Microbial forensic analysis of human-associated bacteria inhabiting hand surface

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