Is Escherichia coli urinary tract infection a zoonosis? Proof of direct link with production animals and meat

Jakobsen, Lotte; Garneau, Philippe; Bruant, Guillaume; Harel, Josée; Olsen, Stefan S.; Porsbo, Lone Jannok; Hammerum, Anette M.; Frimodt‐Møller, Niels

doi:10.1007/s10096-011-1417-5

Cited by 63 publications

(54 citation statements)

References 36 publications

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“…coli is the major causative agent of UTIs in humans and domestic animals [38]. Recent studies suggest that animaloriginating E. coli has zoonotic potential for human infections [10,34]. Therefore, we investigated not only the characteristics of 10 E. coli isolates from dogs with cystitis but also the interaction between the canine UPEC isolates and human bladder epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study on the relationships between human and canine E. coli isolates suggested that these isolates exhibit host specificity with respect to virulence factors [15]. However, several studies have recently shown that E. coli might have zoonotic potential, due to the considerable commonality observed between human and animal E. coli isolates from UTIs [10,12].…”

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confidence: 99%

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Characterization and Zoonotic Potential of Uropathogenic Escherichia coli Isolated from Dogs

Nam

Ko²,

Chae³

et al. 2013

J. Microbiol. Biotechnol.

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The aim of this study was to investigate the characteristics of canine uropathogenic Escherichia coli (UPEC) and the interaction between canine UPEC and human bladder epithelial cells. Ten E. coli isolates collected from dogs with cystitis were analyzed for antimicrobial resistance patterns, the presence of virulence factors, and biofilm formation. The ability of these isolates to induce cytotoxicity, invade human bladder epithelial cells, and stimulate an immune response was also determined. We observed a high rate of antimicrobial resistance among canine UPEC isolates. All virulence genes tested (including adhesins, iron acquisition, and protectin), except toxin genes, were detected among the canine UPEC isolates. We found that all isolates showed varying degrees of biofilm formation (mean, 0.26; range, 0.07 to 0.82), using a microtiter plate assay to evaluate biofilm formation by the isolates. Cytotoxicity to human bladder epithelial cells by the canine UPEC isolates increased in a time-dependent manner, with a 56.9% and 36.1% reduction in cell viability compared with the control at 6 and 9 h of incubation, respectively. We found that most canine UPEC isolates were able to invade human bladder epithelial cells. The interaction between these isolates and human bladder epithelial cells strongly induced the production of proinflammatory cytokines such as IL-6 and IL-8. We demonstrated that canine UPEC isolates can interact with human bladder epithelial cells, although the detailed mechanisms remain unknown. The results suggest that canine UPEC isolates, rather than dogspecific pathogens, have zoonotic potential.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization and Zoonotic Potential of Uropathogenic Escherichia coli Isolated from Dogs

Nam

Ko²,

Chae³

et al. 2013

J. Microbiol. Biotechnol.

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“…The procedure was performed as previously described by Jakobsen et al (32). Briefly, mouse bladders were emptied prior to transurethral inoculation with 50 l of bacterial suspension (10 9 CFU/ml).…”

Section: Methodsmentioning

confidence: 99%

Role of Urinary Cathelicidin LL-37 and Human β-Defensin 1 in Uncomplicated Escherichia coli Urinary Tract Infections

Nielsen

Dynesen²,

Larsen³

et al. 2014

Infect Immun

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; Haslev Laegecenter, Haslev, Denmark c ; Laegehuset Ellemarksvej, Køge, Denmark d Cathelicidin (LL-37) and human ␤-defensin 1 (hBD-1) are important components of the innate defense in the urinary tract. The aim of this study was to characterize whether these peptides are important for developing uncomplicated Escherichia coli urinary tract infections (UTIs). This was investigated by comparing urinary peptide levels of UTI patients during and after infection to those of controls, as well as characterizing the fecal flora of participants with respect to susceptibility to LL-37 and in vivo virulence. Forty-seven UTI patients and 50 controls who had never had a UTI were included. Participants were otherwise healthy, premenopausal, adult women. LL-37 MIC levels were compared for fecal E. coli clones from patients and controls and were also compared based on phylotypes (A, B1, B2, and D). In vivo virulence was investigated in the murine UTI model by use of selected fecal isolates from patients and controls. On average, UTI patients had significantly more LL-37 in urine during infection than postinfection, and patient LL-37 levels postinfection were significantly lower than those of controls. hBD-1 showed similar urine levels for UTI patients and controls. Fecal E. coli isolates from controls had higher LL-37 susceptibility than fecal and UTI E. coli isolates from UTI patients. In vivo studies showed a high level of virulence of fecal E. coli isolates from both patients and controls and showed no difference in virulence correlated with the LL-37 MIC level. The results indicate that the concentration of LL-37 in the urinary tract and low susceptibility to LL-37 may increase the likelihood of UTI in a complex interplay between host and pathogen attributes.

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“…ExPEC virulence genes are common among E. coli isolates from food products, particularly raw meats (5) and especially poultry meat (7). However, although poultry-source E. coli isolates have been shown to cause UTI, sepsis, and meningitis in rodent models that mimic human ExPEC infections (8), the human health risk posed by poultry products is still a matter of some debate, since direct transmission of ExPEC from poultry to humans is difficult to document (4).…”

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confidence: 99%

Zoonotic Potential of Escherichia coli Isolates from Retail Chicken Meat Products and Eggs

Mitchell

Johnson

Johnston

et al. 2015

Appl Environ Microbiol

143

140

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c Chicken products are suspected as a source of extraintestinal pathogenic Escherichia coli (ExPEC), which causes diseases in humans. The zoonotic risk to humans from chicken-source E. coli is not fully elucidated. To clarify the zoonotic risk posed by Ex-PEC in chicken products and to fill existing knowledge gaps regarding ExPEC zoonosis, we evaluated the prevalence of ExPEC on shell eggs and compared virulence-associated phenotypes between ExPEC and non-ExPEC isolates from both chicken meat and eggs. The prevalence of ExPEC among egg-source isolates was low, i.e., 5/108 (4.7%). Based on combined genotypic and phenotypic screening results, multiple human and avian pathotypes were represented among the chicken-source ExPEC isolates, including avian-pathogenic E. coli (APEC), uropathogenic E. coli (UPEC), neonatal meningitis E. coli (NMEC), and sepsis-associated E. coli (SEPEC), as well as an undefined ExPEC group, which included isolates with fewer virulence factors than the APEC, UPEC, and NMEC isolates. These findings document a substantial prevalence of human-pathogenic ExPEC-associated genes and phenotypes among E. coli isolates from retail chicken products and identify key virulence traits that could be used for screening.

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Is Escherichia coli urinary tract infection a zoonosis? Proof of direct link with production animals and meat

Cited by 63 publications

References 36 publications

Characterization and Zoonotic Potential of Uropathogenic Escherichia coli Isolated from Dogs

Characterization and Zoonotic Potential of Uropathogenic Escherichia coli Isolated from Dogs

Role of Urinary Cathelicidin LL-37 and Human β-Defensin 1 in Uncomplicated Escherichia coli Urinary Tract Infections

Zoonotic Potential of Escherichia coli Isolates from Retail Chicken Meat Products and Eggs

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