Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

Abstract

Enzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use the technology to improve enzyme performance, still today we are far from full exploitation of the field.

Construction of a Tannase-Immobilized Magnetic Graphene Oxide/Polymer Nanobiocatalyst with Enhanced Enzyme Stability for High-Efficiency Transformation of Tannins

“…It should be noted that the activity recovery of immobilized tannase is significantly increased in the presence of detergent (Triton X-100). Similar results have been reported in the immobilization of lipases using glutaraldehyde chemistry. − We speculate that the open/close equilibrium of the tannase protein toward the open conformation could be changed by the detergent, and the conformational changes yield an immobilized tannase protein form with an increased activity.…”

Microbial Lipases and Their Potential in the Production of Pharmaceutical Building Blocks

“…The spatial confinement of an enzyme with respect to the reaction bulk is known as enzyme immobilization: it is considered a prerequisite for facilitating the application of enzymes such as lipases in the industry, as it facilitates their reuse by reducing costs, among other benefits [ 41 , 43 , 45 , 51 , 139 ]. The biocatalyst that results from the immobilization process of an enzyme, especially on solid supports, is often referred to as a “derivative”.…”

Plasmonic photothermal activation of an organosilica shielded cold-adapted lipase co-immobilised with gold nanoparticles on silica particles

“…Enzyme activity, stability and substrate promiscuity can also be improved by controlling the enzyme nano-environment. [27][28][29][30][31] In this context, we have focused our efforts on the supramolecular engineering of enzymes immobilised on the surface of silica and shielded in organosilica layers of controlled thickness and chemical composition. [32][33][34][35][36] Among enzymes of interest for industrial applications, cold active enzymes (CAEs) are of particular signicance as they exhibit catalytic activity at low temperatures.…”