Is cortisol a modulator of interferon tau action in the endometrium during early pregnancy in cattle?

Majewska, Magdalena; Lee, Hwa Yong; Tasaki, Yukari; Acosta, Tomás J.; Szóstek, A.Z.; Siemieniuch, Marta; Okuda, Kiyoshi; Skarzynski, Dariusz J.

doi:10.1016/j.jri.2012.01.004

Cited by 19 publications

(17 citation statements)

References 44 publications

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“…Nevertheless, these analyses did show that bovine IFNT as well as CSH gene expressions are identical between AI concepti and SCNT concepti derived from the 5538 cell line (Degrelle et al 2012, Mansouri-Attia et al 2009bVitorino Carvalho A, unpublished observations). Based on recent data reporting the biological functions of embryo signals other than IFNT, glucocorticoids and PGs produced by the trophectoderm and the endometrium are also part of the orchestra that regulates the expression of endometrial genes during implantation in ruminants (Dorniak et al 2011, Majewska et al 2012. Compared with pregnancies obtained by IVP embryos, uterine flushings from bovine SCNT pregnancies were reported to contain lower levels of PGI2 and PGE2 due to a reduced amount of embryoand/or endometrium produced PG (reviewed by Ulbrich et al (2012)).…”

Section: Luminal Epitheliummentioning

confidence: 99%

SOCS genes expression during physiological and perturbed implantation in bovine endometrium

Carvalho¹,

Reinaud²,

Forde³

et al. 2014

Reproduction

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In mammals, suppressor of cytokine signalling (CISH, SOCS1 to SOCS7) factors control signalling pathways involved in the regulation of numerous physiological processes including pregnancy. In order to gain new insights into the biological functions of SOCS in the endometrium, a comprehensive analysis of SOCS gene expression was carried out in bovine caruncular (CAR) and intercaruncular (ICAR) tissues collected i) during the oestrous cycle, ii) at the time of maternal recognition of pregnancy and at implantation in inseminated females, iii) following uterine interferon-tau (IFNT) infusion at day 14 post-oestrus, iv) following a period of controlled intravaginal progesterone release and v) following transfer of embryos by somatic-cell nuclear transfer (SCNT). The regulatory effects of IFNT on in vitro cultured epithelial and stromal cells were also examined. Altogether, our data showed that CISH, SOCS4, SOCS5 and SOCS7 mRNA levels were poorly affected during luteolysis and pregnancy. In contrast, SOCS1, SOCS2, SOCS3 and SOCS6 mRNA levels were strongly up-regulated at implantation (day 20 of pregnancy). Experimental in vitro and in vivo models demonstrated that only CISH, SOCS1, SOCS2 and SOCS3 were IFNT-induced genes. Immunohistochemistry showed an intense SOCS3 and SOCS6 staining in the nucleus of luminal and glandular epithelium and of stromal cells of pregnant endometrium. Finally, SOCS3 expression was significantly increased in SCNT pregnancies in keeping with the altered immune function previously reported in this model of compromised implantation. Collectively, our data suggest that spatio-temporal changes in endometrial SOCS gene expression reflect the acquisition of receptivity, maternal recognition of pregnancy and implantation.Free French abstract A French translation of this abstract is freely available at

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Section: Luminal Epitheliummentioning

confidence: 99%

SOCS genes expression during physiological and perturbed implantation in bovine endometrium

Carvalho¹,

Reinaud²,

Forde³

et al. 2014

Reproduction

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“…Blood samples were collected from the jugular vein on Days 0,6,12,15,16,17,18, 19 and 21. Plasma concentrations of P4 in blood samples were measured.…”

Section: Effects Of Cortisol and Metyrapone Applications Between Daysmentioning

confidence: 99%

“…Access of GCs to GC receptors in target tissues is regulated by two 11β-HSDs, bidirectional 11β-HSD type 1 (11β-HSD1) that mainly converts cortisone to active cortisol [11] and 11β-HSD type 2 (11β-HSD2) that inactivates cortisol to cortisone [12]. Although both 11β-HSDs and GC receptors are expressed in the bovine corpus luteum (CL) [13,14] and endometrium [8] throughout the estrous cycle and early pregnancy [15], the role of GC in regulating CL function is still controversial.Our previous in vitro studies have suggested that cortisol suppresses tumor necrosis factor α (TNFα)-stimulated prostaglandin (PG) F 2α production in endometrial stromal cells [8] and inhibits apoptosis of cultured luteal cells induced by TNFα and interferon-γ (IFNG) [13]. Based on the results of in vitro studies, it seems that cortisol plays a role in preventing excessive uterine PGF 2α production and protecting the CL against apoptosis in nonpregnant cattle [13,16].…”

mentioning

confidence: 99%

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confidence: 99%

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Effects of Cortisol on Pregnancy Rate and Corpus Luteum Function in Heifers: An <i>In Vivo</i> Study

Duong

Piotrowska-Tomala

Acosta

et al. 2012

Journal of Reproduction and Development

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Abstract. To determine whether glucocorticoids affect the function of the bovine corpus luteum (CL) during the estrous cycle and early pregnancy, we examined the effects of exogenous cortisol or reduced endogenous cortisol on the secretion of progesterone (P4) and on pregnancy rate. In preliminary experiments, doses of cortisol and metyrapone (an inhibitor of cortisol synthesis) were established (n=33). Cortisol in effective doses of 10 mg blocked tumor necrosis factor-induced prostaglandin F 2α secretion as measured by its metabolite (PGFM) concentrations in the blood. Metyrapone in effective doses of 500 mg increased the P4 concentration. Thus, both reagents were then intravaginally applied in the chosen doses daily from Day 15 to 18 after estrus (Day 0) in noninseminated heifers (n=18) or after artificial insemination (n=36). Pregnancy was confirmed by transrectal ultrasonography between Days 28-30 after insemination. Plasma concentrations of P4 were lower in cortisol-treated heifers than in control heifers on Days 17 and 18 of the estrous cycle (P<0.05). However, the interestrus intervals were not different between control and cortisol-treated animals (P>0.05). Moreover, metyrapone increased P4 and prolonged the CL lifespan in comparison to control animals (P<0.05). Interestingly, in inseminated heifers, cortisol increased the pregnancy rate (75%) compared with control animals (58%), whereas metyrapone reduced the pregnancy rate to 16.7% (P<0.05). The overall results suggest that cortisol, depending on the physiological status of heifers (pregnant vs. nonpregnant), modulates CL function by influencing P4 secretion. Cortisol may have a positive influence on CL function during early pregnancy, leading to support of embryo implantation and resulting in higher rates of pregnancy in heifers. Key words: Cattle, Cortisol, Early pregnancy, Estrous cycle, Progesterone (J. Reprod. Dev. 58: [223][224][225][226][227][228][229][230] 2012) G lucocorticoids (GCs) are involved in many physiological processes [1,2], including female reproductive functions [3] in rabbits [4], ewes [5] and humans [6]. Cortisol, an active GC, is an anti-inflammatory agent that acts to modulate the production and action of cytokines and prostaglandins required for ovulation, luteolysis, embryo implantation, fetal growth and placental development [3,7]. It is synthesized from cholesterol in the adrenal cortex and is locally regulated by 11β-hydroxysteroid dehydrogenases (11β-HSDs) [8]. The biological action of GCs is mediated through the activation of intracellular GR receptors (GC-R). Two isoforms of GC-R, GC-Rα and GC-Rβ, have been identified [9,10]. Access of GCs to GC receptors in target tissues is regulated by two 11β-HSDs, bidirectional 11β-HSD type 1 (11β-HSD1) that mainly converts cortisone to active cortisol [11] and 11β-HSD type 2 (11β-HSD2) that inactivates cortisol to cortisone [12]. Although both 11β-HSDs and GC receptors are expressed in the bovine corpus luteum (CL) [13,14] and endometrium [8] throughout the estrous cycle and ea...

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“…The GC-GR dimerizes, and then the complex is translocated to the nucleus and binds to specific DNA sequences, the GC response element in target genes, leading to the induction or repression of gene transcription [Lu and Cidlowski, 2006;Kino et al, 2011;Torres Uchoa et al, 2014]. Thus, GCs regulate mRNA expression of inflammatory molecules directly or indirectly through the synthesis of anti-inflammatory proteins, or, more importantly, by transrepression mechanisms [Adcock, 2000;Barnes, 2006;Lu and Cidlowski, 2006;Majewska et al, 2012].…”

Section: Introductionmentioning

confidence: 99%

Role of Glucocorticoids in Cystic Ovarian Disease: Expression of Glucocorticoid Receptor in the Bovine Ovary

Amweg

Rodríguez

Huber³

et al. 2015

Cells Tissues Organs

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The aim of this study was to characterize the expression of glucocorticoid receptor (GR) in the components of normal bovine ovary and in animals with cystic ovarian disease (COD). Changes in the protein and mRNA expression levels were determined in control cows and cows with COD by immunohistochemistry and real-time PCR. GR protein expression in granulosa cells was higher in cysts from animals with spontaneous COD and adrenocorticotropic hormone-induced COD than in tertiary follicles from control animals. In theca interna cells, GR expression was higher in cysts from animals with spontaneous COD than in tertiary follicles from control animals. The increase in GR expression observed in cystic follicles suggests a mechanism of action for cortisol and its receptor through the activation/inactivation of specific transcription factors. These factors could be related to the pathogenesis of COD in cattle.

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Is cortisol a modulator of interferon tau action in the endometrium during early pregnancy in cattle?

Cited by 19 publications

References 44 publications

SOCS genes expression during physiological and perturbed implantation in bovine endometrium

SOCS genes expression during physiological and perturbed implantation in bovine endometrium

Effects of Cortisol on Pregnancy Rate and Corpus Luteum Function in Heifers: An <i>In Vivo</i> Study

Role of Glucocorticoids in Cystic Ovarian Disease: Expression of Glucocorticoid Receptor in the Bovine Ovary

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